Restriction map for
pFN21AE5629
(Linear) (Six-base) MAPSORT of: KIEE5629.seq Check: 7370 from: 1 to: 1413
KIEE5629.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 416 1029 1413
Size: 187 229 613 384
Fragments arranged by size:
613 384 229 187
AfeI AGC'GCT
Cuts at: 0 121 1413
Size: 121 1292
AhdI GACnn_n'nnGTC
Cuts at: 0 47 1413
Size: 47 1366
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 965 1413
Size: 965 448
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 612 644 1413
Size: 612 32 769
Fragments arranged by size:
769 612 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1361 1413
Size: 1361 52
ApaI G_GGCC'C
Cuts at: 0 57 542 1413
Size: 57 485 871
Fragments arranged by size:
871 485 57
ApoI r'AATT_y
Cuts at: 0 348 453 1057 1395 1413
Size: 348 105 604 338 18
Fragments arranged by size:
604 348 338 105 18
BamHI G'GATC_C
Cuts at: 0 782 1413
Size: 782 631
BanI G'GyrC_C
Cuts at: 0 926 1413
Size: 926 487
BanII G_rGCy'C
Cuts at: 0 57 542 1068 1413
Size: 57 485 526 345
Fragments arranged by size:
526 485 345 57
BbeI G_GCGC'C
Cuts at: 0 930 1413
Size: 930 483
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 818 1114 1271 1413
Size: 107 711 296 157 142
Fragments arranged by size:
711 296 157 142 107
BbvCI CC'TCA_GC
Cuts at: 0 938 1413
Size: 938 475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 460 494 1413
Size: 460 34 919
Fragments arranged by size:
919 460 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1161 1413
Size: 1161 252
BfrBI ATG'CAT
Cuts at: 0 3 1413
Size: 3 1410
Bme1580I G_kGCm'C
Cuts at: 0 57 542 1413
Size: 57 485 871
Fragments arranged by size:
871 485 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 62 1126 1413
Size: 62 1064 287
Fragments arranged by size:
1064 287 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 59 1413
Size: 59 1354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 728 1227 1383 1413
Size: 91 637 499 156 30
Fragments arranged by size:
637 499 156 91 30
Bpu10I CC'TnA_GC
Cuts at: 0 938 1413
Size: 938 475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 1413
Size: 54 1359
BsaHI Gr'CG_yC
Cuts at: 0 927 1413
Size: 927 486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1356 1386 1413
Size: 1356 30 27
Fragments arranged by size:
1356 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 437 602 905 1413
Size: 437 165 303 508
Fragments arranged by size:
508 437 303 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1413
Size: 1169 244
BsiHKAI G_wGCw'C
Cuts at: 0 714 1019 1413
Size: 714 305 394
Fragments arranged by size:
714 394 305
BsmI GAATG_Cn'
Cuts at: 0 180 409 1413
Size: 180 229 1004
Fragments arranged by size:
1004 229 180
Bsp1286I G_dGCh'C
Cuts at: 0 57 542 714 1019 1068 1413
Size: 57 485 172 305 49 345
Fragments arranged by size:
485 345 305 172 57 49
BspHI T'CATG_A
Cuts at: 0 269 488 1026 1413
Size: 269 219 538 387
Fragments arranged by size:
538 387 269 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 933 1413
Size: 933 480
BsrDI GCAATG_nn'
Cuts at: 0 476 1413
Size: 476 937
BsrFI r'CCGG_y
Cuts at: 0 739 1413
Size: 739 674
BsrGI T'GTAC_A
Cuts at: 0 774 1413
Size: 774 639
BstYI r'GATC_y
Cuts at: 0 782 1413
Size: 782 631
Bsu36I CC'TnA_GG
Cuts at: 0 681 1413
Size: 681 732
DraI TTT'AAA
Cuts at: 0 909 1413
Size: 909 504
EaeI y'GGCC_r
Cuts at: 0 672 979 1413
Size: 672 307 434
Fragments arranged by size:
672 434 307
EarI CTCTTCn'nnn_
Cuts at: 0 34 1413
Size: 34 1379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 187 416 728 1029 1227 1383 1413
Size: 91 96 229 312 301 198 156 30
Fragments arranged by size:
312 301 229 198 156 96 91 30
EcoO109I rG'GnC_Cy
Cuts at: 0 12 54 539 702 1413
Size: 12 42 485 163 711
Fragments arranged by size:
711 485 163 42 12
FspI TGC'GCA
Cuts at: 0 662 1413
Size: 662 751
HaeII r_GCGC'y
Cuts at: 0 123 930 1413
Size: 123 807 483
Fragments arranged by size:
807 483 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 33 65 76 108 526 558 774 806
Size: 33 32 11 32 418 32 216 32
Cuts at: 806 1413
Size: 607
Fragments arranged by size:
607 418 216 33 32 32 32 32
11
KasI G'GCGC_C
Cuts at: 0 926 1413
Size: 926 487
MscI TGG'CCA
Cuts at: 0 981 1413
Size: 981 432
MslI CAynn'nnrTG
Cuts at: 0 602 1413
Size: 602 811
MspA1I CmG'CkG
Cuts at: 0 1153 1413
Size: 1153 260
NarI GG'CG_CC
Cuts at: 0 927 1413
Size: 927 486
NdeI CA'TA_TG
Cuts at: 0 1011 1413
Size: 1011 402
NsiI A_TGCA'T
Cuts at: 0 5 1413
Size: 5 1408
PflMI CCAn_nnn'nTGG
Cuts at: 0 535 583 1233 1279 1406 1413
Size: 535 48 650 46 127 7
Fragments arranged by size:
650 535 127 48 46 7
PfoI T'CCnGG_A
Cuts at: 0 801 1413
Size: 801 612
PpuMI rG'GwC_Cy
Cuts at: 0 12 702 1413
Size: 12 690 711
Fragments arranged by size:
711 690 12
PspOMI G'GGCC_C
Cuts at: 0 53 538 1413
Size: 53 485 875
Fragments arranged by size:
875 485 53
PvuII CAG'CTG
Cuts at: 0 1153 1413
Size: 1153 260
SexAI A'CCwGG_T
Cuts at: 0 1199 1413
Size: 1199 214
SfcI C'TryA_G
Cuts at: 0 357 1413
Size: 357 1056
SfoI GGC'GCC
Cuts at: 0 928 1413
Size: 928 485
SmlI C'TyrA_G
Cuts at: 0 33 1413
Size: 33 1380
StyI C'CwwG_G
Cuts at: 0 698 1413
Size: 698 715
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 1413
Size: 292 1121
TatI w'GTAC_w
Cuts at: 0 774 1413
Size: 774 639
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 793 1413
Size: 793 620
Enzymes that do cut:
AcuI AfeI AhdI AlfI AloI AlwNI ApaI ApoI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BstYI Bsu36I DraI EaeI EarI
Eco57MI EcoO109I FspI HaeII Hin4I KasI MscI MslI
MspA1I NarI NdeI NsiI PflMI PfoI PpuMI PspOMI
PvuII SexAI SfcI SfoI SmlI StyI TaqII TatI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BclI BglI BglII BlpI BmgBI BmtI BsaI BsaAI
BsaBI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI