Restriction map for
pFN21AE4460
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AspHI AspI AvaI BamHI BanII
BbeI BbrPI BbvCI BclI BcnI
BfrI BglI BglII BmyI BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Cfr10I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HaeII HapII HincII HindII HpaI
HpaII KasI KspI MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 822 GGTACC G^GTACC
AcyI 855 GACGCC G[GA]^CG[CT]C
AlwNI 845 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 954 CCTCAGG CC^T[ACGT]AGG
AseI 571 ATTAAT AT^TAAT
AsnI 571 ATTAAT AT^TAAT
Asp700I 1282 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 822 GGTACC G^GTACC
AviII 260 TGCGCA TGC^GCA
AvrII 1134 CCTAGG C^CTAGG
BanI 822 GGTACC G^G[CT][GA]CC
BcgI 1043 GCAAGGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1134 CCTAGG C^CTAGG
BlpI 44 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 44 GCTCAGC GC^T[ACGT]AGC
BsaHI 855 GACGCC G[GA]^CG[CT]C
BsmI 773 GCATTC GAATGC(1/-1)
BspDI 1162 ATCGAT AT^CGAT
BspHI 651 TCATGA T^CATGA
BstYI 1354 GGATCT [GA]^GATC[CT]
Bsu36I 954 CCTCAGG CC^T[ACGT]AGG
CelII 44 GCTCAGC GC^T[ACGT]AGC
ClaI 1162 ATCGAT AT^CGAT
DraII 1046 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 954 CCTCAGG CC^T[ACGT]AGG
EcoNI 559 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1046 AGGACCT [GA]G^G[ACGT]CC[CT]
FspI 260 TGCGCA TGC^GCA
HgaI 855 GACGC GACGC(5/10)
Hin1I 855 GACGCC G[GA]^CG[CT]C
KpnI 822 GGTACC GGTAC^C
MaeII 287 ACGT A^CGT
MflI 1354 GGATCT [GA]^GATC[CT]
MspA1I 1416 CAGCTG C[AC]G^C[GT]G
MwoI 936 GCACTTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 1250 CCATGG C^CATGG
NdeI 1028 CATATG CA^TATG
PpuMI 1046 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 571 ATTAAT AT^TAAT
PvuII 1416 CAGCTG CAG^CTG
RcaI 651 TCATGA T^CATGA
SmlI 755 CTCAAG C^T[CT][GA]AG
TfiI 391 GATTC G^A[AT]TC
XbaI 111 TCTAGA T^CTAGA
XhoII 1354 GGATCT [GA]^GATC[CT]
XmnI 1282 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 747 CCGC CCGC(-3/-1)
1025 CCGC
AcsI 1245 AAATTC [GA]^AATT[CT]
1327 GAATTT
AlwI 871 GATCC GGATC(4/5)
1354 GGATC
ApoI 1245 AAATTC [GA]^AATT[CT]
1327 GAATTT
BalI 660 TGGCCA TGG^CCA
997 TGGCCA
BbsI 1282 GAAGAC GAAGAC(2/6)
8 GTCTTC
BciVI 615 GGATAC GTATCC(6/5)
1111 GGATAC
BpuAI 1282 GAAGAC GAAGAC(2/6)
8 GTCTTC
BsaI 811 GAGACC GGTCTC(1/5)
673 GGTCTC
BsrI 1223 ACTGG ACTGG(1/-1)
1351 ACTGG
CfoI 261 GCGC GCG^C
945 GCGC
DraI 925 TTTAAA TTT^AAA
1063 TTTAAA
DsaI 827 CCACGG C^C[GA][CT]GG
1250 CCATGG
EaeI 660 TGGCCA [CT]^GGCC[GA]
997 TGGCCA
HhaI 261 GCGC GCG^C
945 GCGC
HinP1I 261 GCGC G^CGC
945 GCGC
HindIII 698 AAGCTT A^AGCTT
961 AAGCTT
MluNI 660 TGGCCA TGG^CCA
997 TGGCCA
MscI 660 TGGCCA TGG^CCA
997 TGGCCA
PshAI 166 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
286 GACGTTGGTC
TaqI 131 TCGA T^CGA
1163 TCGA
TspRI 168 CAGTG CA[GC]TG[ACGT][ACGT]^
693 CACTG
TthHB8I 131 TCGA T^CGA
1163 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AfaI AluI AvaII BbvI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI NdeII NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI Tru9I TseI Tsp45I
Tsp509I Van91I