################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI AseI AsnI Asp700I Asp718I AviII AvrII BbvCI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspLU11I BssSI Bst1107I BstZ17I CelII ClaI DrdI Eco47III EcoRI EcoRV EcoT22I FseI FspI HpaI KpnI MroI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PstI PvuI SapI SbfI ScaI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AhdI 226 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AscI 1399 GGCGCGCC GG^CGCGCC AspEI 226 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 334 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 887 TGGCCA TGG^CCA BamHI 70 GGATCC G^GATCC BcgI 687 CGAGGAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2060 TGATCA T^GATCA BsaBI 2291 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 635 CGTCTC CGTCTC(1/5) Bsp1407I 1385 TGTACA T^GTACA BspHI 491 TCATGA T^CATGA BsrGI 1385 TGTACA T^GTACA BstBI 2216 TTCGAA TT^CGAA BstEII 405 GGTCACC G^GT[ACGT]ACC BstPI 405 GGTCACC G^GT[ACGT]ACC CpoI 499 CGGTCCG CG^G[AT]CCG DraI 1915 TTTAAA TTT^AAA EagI 1319 CGGCCG C^GGCCG Eam1105I 226 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1319 CGGCCG C^GGCCG Eco52I 1319 CGGCCG C^GGCCG EcoO65I 405 GGTCACC G^GT[ACGT]ACC FbaI 2060 TGATCA T^GATCA HincII 1365 GTCGAC GT[CT]^[GA]AC HindII 1365 GTCGAC GT[CT]^[GA]AC HindIII 2428 AAGCTT A^AGCTT MamI 2291 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2564 CAATTG C^AATTG MluI 1115 ACGCGT A^CGCGT MluNI 887 TGGCCA TGG^CCA MscI 887 TGGCCA TGG^CCA MunI 2564 CAATTG C^AATTG NspI 1861 GCATGT [GA]CATG^[CT] NspV 2216 TTCGAA TT^CGAA PflFI 334 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 410 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 491 TCATGA T^CATGA RsrII 499 CGGTCCG CG^G[AT]CCG SalI 1365 GTCGAC G^TCGAC SexAI 2028 ACCAGGT A^CC[AT]GGT SfuI 2216 TTCGAA TT^CGAA SmlI 1588 CTCAAG C^T[CT][GA]AG SspBI 1385 TGTACA T^GTACA StuI 1184 AGGCCT AGG^CCT Tth111I 334 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 410 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 1365 GTCGAC GT^[AC][GT]AC 1609 GTCTAC AcsI 1912 AAATTT [GA]^AATT[CT] 2710 GAATTT AflIII 1115 ACGCGT A^C[GA][CT]GT 2307 ACACGT AocI 362 CCTCAGG CC^T[ACGT]AGG 1632 CCTGAGG ApaI 620 GGGCCC GGGCC^C 1416 GGGCCC ApoI 1912 AAATTT [GA]^AATT[CT] 2710 GAATTT BbrPI 37 CACGTG CAC^GTG 913 CACGTG BbsI 973 GAAGAC GAAGAC(2/6) 1765 GTCTTC BpuAI 973 GAAGAC GAAGAC(2/6) 1765 GTCTTC BsaAI 37 CACGTG [CT]AC^GT[GA] 913 CACGTG BsgI 1538 CTGCAC GTGCAG(16/14) 2741 CTGCAC BsiEI 1055 CGGTCG CG[GA][CT]^CG 1319 CGGCCG BspMI 97 GCAGGT ACCTGC(4/8) 1605 GCAGGT BsrDI 2204 CATTGC GCAATG(2/0) 1780 GCAATG BssHII 1192 GCGCGC G^CGCGC 1400 GCGCGC BstAPI 579 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1948 GCACTGGATGC BstXI 893 CCACGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2753 CCAAGATATTGG Bsu36I 362 CCTCAGG CC^T[ACGT]AGG 1632 CCTGAGG DraIII 1034 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 2699 CACTGAGTG EarI 175 CTCTTC CTCTTC(1/4) 2446 GAAGAG Eco81I 362 CCTCAGG CC^T[ACGT]AGG 1632 CCTGAGG EcoNI 1599 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2491 CCTTTGGAAGG Ksp632I 175 CTCTTC CTCTTC(1/4) 2446 GAAGAG PmaCI 37 CACGTG CAC^GTG 913 CACGTG PmlI 37 CACGTG CAC^GTG 913 CACGTG SacI 2551 GAGCTC GAGCT^C 2595 GAGCTC SfcI 1061 CTGTAG C^T[GA][CT]AG 1128 CTATAG XcmI 833 CCAGGGTCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 890 CCACCACGGTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfoI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI