Restriction map for
pFN21AB9766
(Circular) (Six-base) MAPSORT of: pFN21AB9766.seq Check: 7336 from: 1 to: 7504
pFN21AB9766 7504 bp
With 182 enzymes: SgfI *
August 26, 2010 19:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5314 278
Size: 53 83 186 936 3778 2468
Fragments arranged by size:
3778 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3371 3615 4847 1937
Size: 1434 244 1232 4594
Fragments arranged by size:
4594 1434 1232 244
Acc65I G'GTAC_C
Cuts at: 4825 4825
Size: 7504
AclI AA'CG_TT
Cuts at: 1563 5631 6004 1563
Size: 4068 373 3063
Fragments arranged by size:
4068 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 2163 2295 2735 4755 5567 6951 7219
Size: 404 127 132 440 2020 812 1384 268
Cuts at: 7219 1632
Size: 1917
Fragments arranged by size:
2020 1917 1384 812 440 404 268 132
127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7307
AflIII A'CryG_T
Cuts at: 1203 3120 4312 6403 7380 1203
Size: 1917 1192 2091 977 1327
Fragments arranged by size:
2091 1917 1327 1192 977
AgeI A'CCGG_T
Cuts at: 6335 6335
Size: 7504
AhdI GACnn_n'nnGTC
Cuts at: 2236 6233 2236
Size: 3997 3507
AleI CACnn'nnGTG
Cuts at: 1166 2086 2900 4317 1166
Size: 920 814 1417 4353
Fragments arranged by size:
4353 1417 920 814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2670 1184
Size: 1486 6018
AlwNI CAG_nnn'CTG
Cuts at: 2242 2590 2974 4330 5247 6819 2242
Size: 348 384 1356 917 1572 2927
Fragments arranged by size:
2927 1572 1356 917 384 348
ApaI G_GGCC'C
Cuts at: 2629 3425 2629
Size: 796 6708
ApaLI G'TGCA_C
Cuts at: 5561 6717 5561
Size: 1156 6348
ApoI r'AATT_y
Cuts at: 1493 3917 4715 4813 5039 5191 7300 1493
Size: 2424 798 98 226 152 2109 1697
Fragments arranged by size:
2424 2109 1697 798 226 152 98
AscI GG'CGCG_CC
Cuts at: 3405 3405
Size: 7504
AseI AT'TA_AT
Cuts at: 160 1032 6058 160
Size: 872 5026 1606
Fragments arranged by size:
5026 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7504
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 2066 2113 2375 2562 2727 2876
Size: 73 88 35 47 262 187 165 149
Cuts at: 2876 3305 3364 4698 4829 1870
Size: 429 59 1334 131 4545
Fragments arranged by size:
4545 1334 429 262 187 165 149 131
88 73 59 47 35
BamHI G'GATC_C
Cuts at: 2075 4834 5201 2075
Size: 2759 367 4378
Fragments arranged by size:
4378 2759 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2362 2424 2872 3227 3448
Size: 325 217 627 575 62 448 355 221
Cuts at: 3448 4825 6280 618
Size: 1377 1455 1842
Fragments arranged by size:
1842 1455 1377 627 575 448 355 325
221 217 62
BanII G_rGCy'C
Cuts at: 1391 2038 2612 2629 2734 3111 3425 3564 3668
Size: 647 574 17 105 377 314 139 104
Cuts at: 3668 4560 4604 4823 1391
Size: 892 44 219 4072
Fragments arranged by size:
4072 892 647 574 377 314 219 139
105 104 44 17
BbeI G_GCGC'C
Cuts at: 2366 2876 3452 2366
Size: 510 576 6418
Fragments arranged by size:
6418 576 510
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2985 3763 928
Size: 971 1086 778 4669
Fragments arranged by size:
4669 1086 971 778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2681 2715 5695 5729 1903
Size: 34 744 34 2980 34 3678
Fragments arranged by size:
3678 2980 744 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5396 6606 5396
Size: 1210 6294
BclI T'GATC_A
Cuts at: 1548 4065 1548
Size: 2517 4987
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2433 2951 3521
Size: 107 122 71 631 605 761 518 570
Cuts at: 3521 3524 6115 136
Size: 3 2591 1525
Fragments arranged by size:
2591 1525 761 631 605 570 518 122
107 71 3
BglII A'GATC_T
Cuts at: 7499 7499
Size: 7504
BlpI GC'TnA_GC
Cuts at: 4921 4921
Size: 7504
Bme1580I G_kGCm'C
Cuts at: 1790 2629 3425 4705 5565 6721 1790
Size: 839 796 1280 860 1156 2573
Fragments arranged by size:
2573 1280 1156 860 839 796
BmgBI CAC'GTC
Cuts at: 1318 2251 4315 1318
Size: 933 2064 4507
Fragments arranged by size:
4507 2064 933
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2624 4516 5235
Size: 416 53 83 399 24 1207 1892 719
Cuts at: 5235 6193 442
Size: 958 1753
Fragments arranged by size:
1892 1753 1207 958 719 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7504
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 7504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2313 2772 3398 4120 6164 1239
Size: 1074 459 626 722 2044 2579
Fragments arranged by size:
2579 2044 1074 722 626 459
Bpu10I CC'TnA_GC
Cuts at: 4010 4010
Size: 7504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3578 5629 6494 6792 3578
Size: 2051 865 298 4290
Fragments arranged by size:
4290 2051 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6167 882
Size: 617 4668 2219
Fragments arranged by size:
4668 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2044 2920 5295 493
Size: 711 840 876 2375 2702
Fragments arranged by size:
2702 2375 876 840 711
BsaBI GATnn'nnATC
Cuts at: 4300 7498 4300
Size: 3198 4306
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2363 2796 2873 3102
Size: 53 83 186 936 830 433 77 229
Cuts at: 3102 3449 5311 5693 6328 275
Size: 347 1862 382 635 1451
Fragments arranged by size:
1862 1451 936 830 635 433 382 347
229 186 83 77 53
BsaWI w'CCGG_w
Cuts at: 5937 6335 6609 6756 7167 5937
Size: 398 274 147 411 6274
Fragments arranged by size:
6274 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4523 4553 1178
Size: 30 165 30 3120 30 4129
Fragments arranged by size:
4129 3120 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2684 2687 2708 2769 2772 3185 3188 3352
Size: 1498 3 21 61 3 413 3 164
Cuts at: 3352 4544 1186
Size: 1192 4146
Fragments arranged by size:
4146 1498 1192 413 164 61 21 3
3 3
BseYI C'CCAG_C
Cuts at: 2095 2390 2475 3414 3862 6707 7203 2095
Size: 295 85 939 448 2845 496 2396
Fragments arranged by size:
2845 2396 939 496 448 295 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3528 4731 1163
Size: 573 1792 1203 3936
Fragments arranged by size:
3936 1792 1203 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3063 3327 4954 5715 6743 1540
Size: 275 186 1062 264 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1062 1028 761 275 264 186
BsiHKAI G_wGCw'C
Cuts at: 3594 3955 4560 4604 5565 5650 6721 3594
Size: 361 605 44 961 85 1071 4377
Fragments arranged by size:
4377 1071 961 605 361 85 44
BsmI GAATG_Cn'
Cuts at: 5015 5108 5015
Size: 93 7411
BsmBI CGTCTCn'nnnn_
Cuts at: 2646 2646
Size: 7504
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2612 2629 2734 3111 3425 3564
Size: 399 248 574 17 105 377 314 139
Cuts at: 3564 3594 3668 3955 4560 4604 4705 4823 5565
Size: 30 74 287 605 44 101 118 742
Cuts at: 5565 5650 6721 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 605 574 399 377 314
287 248 139 118 105 101 85 74
44 30 17
BspEI T'CCGG_A
Cuts at: 7167 7167
Size: 7504
BspHI T'CATG_A
Cuts at: 2496 5393 7123 2496
Size: 2897 1730 2877
Fragments arranged by size:
2897 2877 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2093 3601 4859 844
Size: 1249 1508 1258 3489
Fragments arranged by size:
3489 1508 1258 1249
BsrBI CCG'CTC
Cuts at: 1394 1823 2494 3155 3462 5391 1394
Size: 429 671 661 307 1929 3507
Fragments arranged by size:
3507 1929 671 661 429 307
BsrDI GCAATG_nn'
Cuts at: 65 1248 3792 4208 5999 6173 65
Size: 1183 2544 416 1791 174 1396
Fragments arranged by size:
2544 1791 1396 1183 416 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2458 2502 3224 6148 6335 1700
Size: 110 648 44 722 2924 187 2869
Fragments arranged by size:
2924 2869 722 648 187 110 44
BsrGI T'GTAC_A
Cuts at: 96 3390 96
Size: 3294 4210
BssHII G'CGCG_C
Cuts at: 1925 3197 3405 1925
Size: 1272 208 6024
Fragments arranged by size:
6024 1272 208
BssSI C'ACGA_G
Cuts at: 5564 6576 5564
Size: 1012 6492
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2590 3959 794
Size: 1796 1369 4339
Fragments arranged by size:
4339 1796 1369
BstBI TT'CG_AA
Cuts at: 4222 6308 4222
Size: 2086 5418
BstEII G'GTnAC_C
Cuts at: 1184 2410 1184
Size: 1226 6278
BstXI CCAn_nnnn'nTGG
Cuts at: 2905 4765 2905
Size: 1860 5644
BstYI r'GATC_y
Cuts at: 1972 2075 2452 4301 4413 4834 5201 5586 5603
Size: 103 377 1849 112 421 367 385 17
Cuts at: 5603 7055 7141 7153 7499 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1849 1452 421 385 377 367 346
112 103 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2368 3638 2368
Size: 1270 6234
BtgI C'CryG_G
Cuts at: 513 1065 2763 2898 3029 3476 513
Size: 552 1698 135 131 447 4541
Fragments arranged by size:
4541 1698 552 447 135 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6765
Fragments arranged by size:
6765 683 56
BtsI GCAGTG_nn'
Cuts at: 2081 5020 5814 5834 2081
Size: 2939 794 20 3751
Fragments arranged by size:
3751 2939 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7504
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7469
DraI TTT'AAA
Cuts at: 3922 4808 3922
Size: 886 6618
DraIII CAC_nnn'GTG
Cuts at: 3044 4709 3044
Size: 1665 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6511 809
Size: 507 5195 1802
Fragments arranged by size:
5195 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2892 3222
Size: 54 1425 50 275 18 321 741 330
Cuts at: 3222 3324 4951 5840 7469 8
Size: 102 1627 889 1629 43
Fragments arranged by size:
1629 1627 1425 889 741 330 321 275
102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3324 4951 1537
Size: 275 1512 1627 4090
Fragments arranged by size:
4090 1627 1512 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 4446 5434 1353
Size: 833 2260 988 3423
Fragments arranged by size:
3423 2260 988 833
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3490 6091 6465 6611 387
Size: 1064 138 1901 2601 374 146 1280
Fragments arranged by size:
2601 1901 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4558 4602 4558
Size: 44 7460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2163 2295 2313 2735 2772 3398
Size: 393 404 127 132 18 422 37 626
Cuts at: 3398 4120 4755 5567 6164 6951 7219 1239
Size: 722 635 812 597 787 268 1524
Fragments arranged by size:
1524 812 787 722 635 626 597 422
404 393 268 132 127 37 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3608 4500 3608
Size: 892 6612
EcoO109I rG'GnC_Cy
Cuts at: 2027 2206 2372 2566 2625 2626 3421 3601 2027
Size: 179 166 194 59 1 795 180 5930
Fragments arranged by size:
5930 795 194 180 179 166 59 1
EcoRI G'AATT_C
Cuts at: 4813 5191 4813
Size: 378 7126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3790 3790
Size: 7504
FspI TGC'GCA
Cuts at: 6010 6010
Size: 7504
HaeII r_GCGC'y
Cuts at: 1678 2366 2837 2876 2884 3018 3452 6651 1678
Size: 688 471 39 8 134 434 3199 2531
Fragments arranged by size:
3199 2531 688 471 434 134 39 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2222 2254 3710
Size: 32 135 32 488 32 130 32 1456
Cuts at: 3710 3742 4523 4545 4555 4577 6145 6177 6219
Size: 32 781 22 10 22 1568 32 42
Cuts at: 6219 6251 1373
Size: 32 2626
Fragments arranged by size:
2626 1568 1456 781 488 135 130 42
32 32 32 32 32 32 32 22
22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3372 4848 5094 669
Size: 988 281 1434 1476 246 3079
Fragments arranged by size:
3079 1476 1434 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4433 748
Size: 3685 3819
HpaI GTT'AAC
Cuts at: 5094 5094
Size: 7504
KasI G'GCGC_C
Cuts at: 2362 2872 3448 2362
Size: 510 576 6418
Fragments arranged by size:
6418 576 510
KpnI G_GTAC'C
Cuts at: 4829 4829
Size: 7504
MfeI C'AATT_G
Cuts at: 4569 5103 4569
Size: 534 6970
MluI A'CGCG_T
Cuts at: 3120 7380 3120
Size: 4260 3244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2053 2226 2238 2500 6618 6802 1305
Size: 748 173 12 262 4118 184 2007
Fragments arranged by size:
4118 2007 748 262 184 173 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2894 10
Size: 54 1425 343 1062 4620
Fragments arranged by size:
4620 1425 1062 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2086 2900 2903 2918 3741 4236 4317
Size: 648 920 814 3 15 823 495 81
Cuts at: 4317 5462 5821 5980 518
Size: 1145 359 159 2042
Fragments arranged by size:
2042 1145 920 823 814 648 495 359
159 81 15 3
MspA1I CmG'CkG
Cuts at: 1606 2328 2479 2543 2587 2654 2765 2890 2971
Size: 722 151 64 44 67 111 125 81
Cuts at: 2971 3031 3277 3478 4366 4920 5597 6745 6990
Size: 60 246 201 888 554 677 1148 245
Cuts at: 6990 1606
Size: 2120
Fragments arranged by size:
2120 1148 888 722 677 554 246 245
201 151 125 111 81 67 64 60
44
NarI GG'CG_CC
Cuts at: 2363 2873 3449 2363
Size: 510 576 6418
Fragments arranged by size:
6418 576 510
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6952
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7504
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7504
NotI GC'GGCC_GC
Cuts at: 4951 4951
Size: 7504
NspI r_CATG'y
Cuts at: 1132 1229 3870 4862 6407 1132
Size: 97 2641 992 1545 2229
Fragments arranged by size:
2641 2229 1545 992 97
PasI CC'CwG_GG
Cuts at: 1348 2838 1348
Size: 1490 6014
PciI A'CATG_T
Cuts at: 6403 6403
Size: 7504
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2421 1108
Size: 1313 6191
PfoI T'CCnGG_A
Cuts at: 2531 3246 2531
Size: 715 6789
PmeI GTTT'AAAC
Cuts at: 4808 4808
Size: 7504
PmlI CAC'GTG
Cuts at: 2044 2920 2044
Size: 876 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5573 5605 7086 7118 5573
Size: 32 1481 32 5959
Fragments arranged by size:
5959 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 2206 2372 2027
Size: 179 166 7159
Fragments arranged by size:
7159 179 166
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7504
PsiI TTA'TAA
Cuts at: 5074 5074
Size: 7504
PspOMI G'GGCC_C
Cuts at: 2625 3421 2625
Size: 796 6708
PstI C_TGCA'G
Cuts at: 830 4856 830
Size: 4026 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7504
PvuII CAG'CTG
Cuts at: 2543 2587 2971 2543
Size: 44 384 7076
Fragments arranged by size:
7076 384 44
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 7504
SacI G_AGCT'C
Cuts at: 4560 4604 4560
Size: 44 7460
SacII CC_GC'GG
Cuts at: 2766 3032 3479 2766
Size: 266 447 6791
Fragments arranged by size:
6791 447 266
SalI G'TCGA_C
Cuts at: 1936 3370 4846 1936
Size: 1434 1476 4594
Fragments arranged by size:
4594 1476 1434
SanDI GG'GwC_CC
Cuts at: 2027 2027
Size: 7504
SbfI CC_TGCA'GG
Cuts at: 4856 4856
Size: 7504
ScaI AGT'ACT
Cuts at: 5752 7464 5752
Size: 1712 5792
SexAI A'CCwGG_T
Cuts at: 4033 4033
Size: 7504
SfcI C'TryA_G
Cuts at: 826 1045 3066 3133 4852 5987 6668 6859 826
Size: 219 2021 67 1719 1135 681 191 1471
Fragments arranged by size:
2021 1719 1471 1135 681 219 191 67
SfoI GGC'GCC
Cuts at: 2364 2874 3450 2364
Size: 510 576 6418
Fragments arranged by size:
6418 576 510
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7504
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7504
SmaI CCC'GGG
Cuts at: 1872 2033 2115 2377 2564 2878 3307 4700 4831
Size: 161 82 262 187 314 429 1393 131
Cuts at: 4831 1872
Size: 4545
Fragments arranged by size:
4545 1393 429 314 262 187 161 131
82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3593 5608 6509 6771 820
Size: 197 926 1650 2015 901 262 1553
Fragments arranged by size:
2015 1650 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5295 493
Size: 4802 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7504
SphI G_CATG'C
Cuts at: 1132 4862 1132
Size: 3730 3774
SrfI GCCC'GGGC
Cuts at: 2878 2878
Size: 7504
SspI AAT'ATT
Cuts at: 5 52 5428 5
Size: 47 5376 2081
Fragments arranged by size:
5376 2081 47
StuI AGG'CCT
Cuts at: 1517 3191 1517
Size: 1674 5830
StyI C'CwwG_G
Cuts at: 513 1065 2811 3111 3904 4943 513
Size: 552 1746 300 793 1039 3074
Fragments arranged by size:
3074 1746 1039 793 552 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2489 2726 5531 5548 5701 5886 6331
Size: 1255 909 237 2805 17 153 185 445
Cuts at: 6331 325
Size: 1498
Fragments arranged by size:
2805 1498 1255 909 445 237 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3390 4671 5750
Size: 274 80 33 51 1443 1413 1281 1079
Cuts at: 5750 7462 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1413 1281 1079 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7464 7496 7464
Size: 32 7472
Tth111I GACn'n_nGTC
Cuts at: 1316 2342 1316
Size: 1026 6478
XbaI T'CTAG_A
Cuts at: 4840 4840
Size: 7504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2845 2902 2845
Size: 57 7447
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7504
XmaI C'CCGG_G
Cuts at: 1870 2031 2113 2375 2562 2876 3305 4698 4829
Size: 161 82 262 187 314 429 1393 131
Cuts at: 4829 1870
Size: 4545
Fragments arranged by size:
4545 1393 429 314 262 187 161 131
82
XmnI GAAnn'nnTTC
Cuts at: 5633 5633
Size: 7504
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5312 276
Size: 53 83 186 936 3778 2468
Fragments arranged by size:
3778 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI SapI SfiI SwaI