Restriction map for
pFN21AE2423
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII AccII AccIII AciI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssKI BssSI BstAPI BstEII
BstNI BstPI BstUI BstXI Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvaI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI ScrFI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 293 GGTACC G^GTACC
AccI 739 GTATAC GT^[AC][GT]AC
AclI 450 AACGTT AA^CGTT
Asp718I 293 GGTACC G^GTACC
BamHI 607 GGATCC G^GATCC
BanI 293 GGTACC G^G[CT][GA]CC
BciVI 107 GTATCC GTATCC(6/5)
BsrI 811 ACTGG ACTGG(1/-1)
Bst1107I 739 GTATAC GTA^TAC
BstBI 146 TTCGAA TT^CGAA
BstZ17I 739 GTATAC GTA^TAC
Cac8I 459 GCAAGC GC[ACGT]^[ACGT]GC
DraI 94 TTTAAA TTT^AAA
DsaI 313 CCACGG C^C[GA][CT]GG
EcoRI 718 GAATTC G^AATTC
EcoRV 679 GATATC GAT^ATC
EcoT14I 433 CCTTGG C^C[AT][AT]GG
HaeIII 823 GGCC GG^CC
HincII 630 GTCAAC GT[CT]^[GA]AC
HindII 630 GTCAAC GT[CT]^[GA]AC
KpnI 293 GGTACC GGTAC^C
MaeII 451 ACGT A^CGT
MslI 178 CACGCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 793 GCTACAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 31 CATG CATG^
NspV 146 TTCGAA TT^CGAA
Psp1406I 450 AACGTT AA^CGTT
SfuI 146 TTCGAA TT^CGAA
StuI 822 AGGCCT AGG^CCT
StyI 433 CCTTGG C^C[AT][AT]GG
TspRI 103 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 161 GTAC GT^AC
294 GTAC
Asp700I 14 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
169 GAAAGTGTTC
BbsI 308 GAAGAC GAAGAC(2/6)
320 GAAGAC
BbvI 86 GCAGC GCAGC(8/12)
772 GCTGC
BpuAI 308 GAAGAC GAAGAC(2/6)
320 GAAGAC
BsaJI 313 CCACGG C^C[ACGT][ACGT]GG
433 CCTTGG
BseRI 288 GAGGAG GAGGAG(10/8)
625 GAGGAG
BstYI 388 GGATCT [GA]^GATC[CT]
607 GGATCC
DpnII 389 GATC ^GATC
608 GATC
EarI 75 CTCTTC CTCTTC(1/4)
751 GAAGAG
Fnu4HI 86 GCAGC GC^[ACGT]GC
772 GCTGC
HphI 547 GGTGA GGTGA(8/7)
118 TCACC
ItaI 86 GCAGC GC^[ACGT]GC
772 GCTGC
Ksp632I 75 CTCTTC CTCTTC(1/4)
751 GAAGAG
MaeIII 34 GTGAC ^GT[ACGT]AC
197 GTGAC
MboI 389 GATC ^GATC
608 GATC
MflI 388 GGATCT [GA]^GATC[CT]
607 GGATCC
NdeII 389 GATC ^GATC
608 GATC
NlaIV 293 GGTACC GG[ACGT]^[ACGT]CC
607 GGATCC
PleI 199 GACTC GAGTC(4/5)
628 GAGTC
RsaI 161 GTAC GT^AC
294 GTAC
Sau3AI 389 GATC ^GATC
608 GATC
SfaNI 43 GATGC GCATC(5/9)
649 GATGC
TaqI 63 TCGA T^CGA
147 TCGA
TseI 86 GCAGC G^C[AT]GC
772 GCTGC
Tsp45I 34 GTGAC ^GT[GC]AC
197 GTGAC
TthHB8I 63 TCGA T^CGA
147 TCGA
XhoII 388 GGATCT [GA]^GATC[CT]
607 GGATCC
XmnI 14 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
169 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AcsI AluI AlwI ApoI BstF5I
DdeI FokI HinfI MboII MnlI
MseI TfiI Tru9I Tsp509I