################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MroI MunI NarI NdeI NheI NlaIV NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 43 CGCG CG^CG AclI 718 AACGTT AA^CGTT AcyI 15 GACGCC G[GA]^CG[CT]C Asp700I 299 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 1280 TGGCCA TGG^CCA BclI 162 TGATCA T^GATCA BcnI 29 CCGGG CC^[GC]GG BfaI 52 CTAG C^TAG BglI 28 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1116 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 15 GACGCC G[GA]^CG[CT]C BseRI 46 GAGGAG GAGGAG(10/8) BsiYI 870 CCTTCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 870 CCTTCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 100 GGGAC GGGAC(10/14) BsmI 1369 GCATTC GAATGC(1/-1) Bsp1407I 183 TGTACA T^GTACA BspHI 543 TCATGA T^CATGA BsrFI 18 GCCGGC [GA]^CCGG[CT] BsrGI 183 TGTACA T^GTACA BstBI 1320 TTCGAA TT^CGAA BstNI 555 CCTGG CC^[AT]GG BstUI 43 CGCG CG^CG CfoI 42 GCGC GCG^C Cfr10I 18 GCCGGC [GA]^CCGG[CT] DraII 70 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 1280 TGGCCA [CT]^GGCC[GA] EcoO109I 70 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 1482 GAATTC G^AATTC EcoRII 555 CCTGG ^CC[AT]GG FbaI 162 TGATCA T^GATCA HgaI 15 GACGC GACGC(5/10) HhaI 42 GCGC GCG^C Hin1I 15 GACGCC G[GA]^CG[CT]C HinP1I 42 GCGC G^CGC HphI 477 GGTGA GGTGA(8/7) MaeI 52 CTAG C^TAG MamI 1116 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1280 TGGCCA TGG^CCA MscI 1280 TGGCCA TGG^CCA MspA1I 487 CAGCTG C[AC]G^C[GT]G MvaI 555 CCTGG CC^[AT]GG MvnI 43 CGCG CG^CG NaeI 18 GCCGGC GCC^GGC NciI 29 CCGGG CC^[GC]GG NgoMIV 18 GCCGGC G^CCGGC NspV 1320 TTCGAA TT^CGAA Psp1406I 718 AACGTT AA^CGTT PvuII 487 CAGCTG CAG^CTG RcaI 543 TCATGA T^CATGA SfuI 1320 TTCGAA TT^CGAA SgrAI 17 CGCCGGCG C[GA]^CCGG[CT]G SmlI 911 CTCAAG C^T[CT][GA]AG SspBI 183 TGTACA T^GTACA TaqI 1321 TCGA T^CGA TthHB8I 1321 TCGA T^CGA XcmI 716 CCAACGTTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 299 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BpmI 556 CTGGAG CTGGAG(16/14) 1376 CTGGAG BssKI 29 CCGGG ^CC[ACGT]GG 555 CCTGG BstYI 777 AGATCC [GA]^GATC[CT] 1183 AGATCC DdeI 355 CTGAG C^T[ACGT]AG 519 CTTAG DraI 361 TTTAAA TTT^AAA 1014 TTTAAA DsaI 725 CCATGG C^C[GA][CT]GG 1125 CCATGG MaeII 333 ACGT A^CGT 719 ACGT MflI 777 AGATCC [GA]^GATC[CT] 1183 AGATCC MslI 858 CATAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1074 CATTTTTGTG NcoI 725 CCATGG C^CATGG 1125 CCATGG NspI 770 ACATGC [GA]CATG^[CT] 1489 ACATGC PleI 414 GAGTC GAGTC(4/5) 1464 GAGTC ScrFI 29 CCGGG CC^[ACGT]GG 555 CCTGG SfcI 498 CTGTAG C^T[GA][CT]AG 1364 CTATAG XhoII 777 AGATCC [GA]^GATC[CT] 1183 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbsI BbvI BpuAI BsaJI BsrI BstF5I Cac8I Cfr13I DpnII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII ItaI MaeIII MboI MboII MnlI MseI MspI MwoI NdeII NlaIII RsaI Sau3AI Sau96I SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI