(Circular) (Six-base) MAPSORT of: pFN21AE6470.seq Check: 5571 from: 1 to: 6217 pFN21AE6470 6217 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 2724 4344 4717 1563 Size: 1161 1620 373 3063 Fragments arranged by size: 3063 1620 1161 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 4280 5664 5932 1632 Size: 788 1860 1384 268 1917 Fragments arranged by size: 1917 1860 1384 788 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 3960 5532 3960 Size: 1572 4645 ApaLI G'TGCA_C Cuts at: 4274 5430 4274 Size: 1156 5061 ApoI r'AATT_y Cuts at: 1493 2095 3060 3146 3176 3312 3481 3487 3526 Size: 602 965 86 30 136 169 6 39 Cuts at: 3526 3752 3904 6013 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 965 602 226 169 152 136 86 39 30 6 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 3542 1870 Size: 73 1599 4545 Fragments arranged by size: 4545 1599 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2829 2862 2829 Size: 33 6184 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3538 4993 618 Size: 325 217 627 1751 1455 1842 Fragments arranged by size: 1842 1751 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3536 1391 Size: 2145 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2426 2512 2668 928 Size: 971 527 86 156 4477 Fragments arranged by size: 4477 971 527 156 86 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 2167 1548 Size: 619 5598 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 4828 136 Size: 107 122 71 631 605 367 2789 1525 Fragments arranged by size: 2789 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 3634 3634 Size: 6217 Bme1580I G_kGCm'C Cuts at: 1790 4278 5434 1790 Size: 2488 1156 2573 Fragments arranged by size: 2573 2488 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3948 4906 442 Size: 416 53 83 399 24 2531 958 1753 Fragments arranged by size: 2531 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2582 3402 4877 1239 Size: 1343 820 1475 2579 Fragments arranged by size: 2579 1475 1343 820 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2901 4342 5207 5505 2901 Size: 1441 865 298 3613 Fragments arranged by size: 3613 1441 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 3125 6211 3125 Size: 3086 3131 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 4024 4406 5041 Size: 53 83 186 936 488 2003 382 635 Cuts at: 5041 275 Size: 1451 Fragments arranged by size: 2003 1451 936 635 488 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5992 Fragments arranged by size: 5992 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 5337 BseYI C'CCAG_C Cuts at: 2494 5420 5916 2494 Size: 2926 496 2795 Fragments arranged by size: 2926 2795 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5644 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4278 4363 5434 4278 Size: 85 1071 5061 Fragments arranged by size: 5061 1071 85 BsmI GAATG_Cn' Cuts at: 3374 3728 3821 3374 Size: 354 93 5770 Fragments arranged by size: 5770 354 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3536 4278 4363 5434 1391 Size: 399 1746 742 85 1071 2174 Fragments arranged by size: 2174 1746 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 2548 4106 5836 2548 Size: 1558 1730 2929 Fragments arranged by size: 2929 1730 1558 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3572 844 Size: 2728 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 4861 5048 1700 Size: 110 213 2838 187 2869 Fragments arranged by size: 2869 2838 213 187 110 BsrGI T'GTAC_A Cuts at: 96 2188 96 Size: 2092 4125 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6217 BstBI TT'CG_AA Cuts at: 3326 5021 3326 Size: 1695 4522 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstYI r'GATC_y Cuts at: 1972 2782 3188 3547 3914 4299 4316 5768 5854 Size: 810 406 359 367 385 17 1452 86 Cuts at: 5854 5866 6212 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 810 406 385 367 359 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2730 3130 513 Size: 552 939 726 400 3600 Fragments arranged by size: 3600 939 726 552 400 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 3733 4527 4547 3733 Size: 794 20 5403 Fragments arranged by size: 5403 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 2368 3021 3521 2368 Size: 653 500 5064 Fragments arranged by size: 5064 653 500 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3285 3664 4553 Size: 54 1425 50 275 18 1455 379 889 Cuts at: 4553 6182 8 Size: 1629 43 Fragments arranged by size: 1629 1455 1425 889 379 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4147 1353 Size: 2794 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2473 4804 5178 5324 387 Size: 1064 138 884 2331 374 146 1280 Fragments arranged by size: 2331 1280 1064 884 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2582 3402 4280 4877 5664 5932 Size: 393 788 162 820 878 597 787 268 Cuts at: 5932 1239 Size: 1524 Fragments arranged by size: 1524 878 820 788 787 597 393 268 162 EcoO109I rG'GnC_Cy Cuts at: 2076 2076 Size: 6217 EcoRI G'AATT_C Cuts at: 3487 3526 3904 3487 Size: 39 378 5800 Fragments arranged by size: 5800 378 39 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 5364 1678 Size: 3686 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 4858 4890 4932 Size: 32 135 32 521 32 2733 32 42 Cuts at: 4932 4964 1373 Size: 32 2626 Fragments arranged by size: 2733 2626 521 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2376 2470 2626 3561 3807 669 Size: 988 281 438 94 156 935 246 3079 Fragments arranged by size: 3079 988 935 438 281 246 156 94 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2792 3241 5331 5515 1305 Size: 1487 449 2090 184 2007 Fragments arranged by size: 2090 2007 1487 449 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3287 10 Size: 54 1425 343 1455 2940 Fragments arranged by size: 2940 1455 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2867 3083 4175 4534 4693 518 Size: 648 1701 216 1092 359 159 2042 Fragments arranged by size: 2042 1701 1092 648 359 216 159 MspA1I CmG'CkG Cuts at: 1606 2494 3633 4310 5458 5703 1606 Size: 888 1139 677 1148 245 2120 Fragments arranged by size: 2120 1148 1139 888 677 245 NaeI GCC'GGC Cuts at: 2025 2025 Size: 6217 NcoI C'CATG_G Cuts at: 513 1065 2004 2730 3130 513 Size: 552 939 726 400 3600 Fragments arranged by size: 3600 939 726 552 400 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 2023 2023 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2779 3498 3575 5120 1132 Size: 97 1550 719 77 1545 2229 Fragments arranged by size: 2229 1550 1545 719 97 77 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6217 PfoI T'CCnGG_A Cuts at: 2559 2559 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 2663 3339 3787 2663 Size: 676 448 5093 Fragments arranged by size: 5093 676 448 PstI C_TGCA'G Cuts at: 830 3569 830 Size: 2739 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2494 2494 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2503 3369 3565 4700 5381 5572 826 Size: 219 1458 866 196 1135 681 191 1471 Fragments arranged by size: 1471 1458 1135 866 681 219 196 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 2023 1700 Size: 323 5894 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2916 4321 5222 5484 820 Size: 197 926 973 1405 901 262 1553 Fragments arranged by size: 1553 1405 973 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 2221 2929 3364 4141 5 Size: 47 2169 708 435 777 2081 Fragments arranged by size: 2169 2081 777 708 435 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6217 StyI C'CwwG_G Cuts at: 513 1065 2004 2423 2730 3130 3656 513 Size: 552 939 419 307 400 526 3074 Fragments arranged by size: 3074 939 552 526 419 400 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4244 4261 4414 4599 5044 325 Size: 1255 2664 17 153 185 445 1498 Fragments arranged by size: 2664 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2188 2704 4463 Size: 274 80 33 51 1443 211 516 1759 Cuts at: 4463 6175 96 Size: 1712 138 Fragments arranged by size: 1759 1712 1443 516 274 211 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6177 6209 6177 Size: 32 6185 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2728 2728 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 2308 4346 2308 Size: 2038 4179 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI