(Linear) (Six-base) MAPSORT of: KIEE6470.seq Check: 4438 from: 1 to: 1512 KIEE6470.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AclI AA'CG_TT Cuts at: 0 719 1512 Size: 719 793 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 1512 Size: 415 1097 ApoI r'AATT_y Cuts at: 0 90 1055 1141 1171 1307 1476 1482 1512 Size: 90 965 86 30 136 169 6 30 Fragments arranged by size: 965 169 136 90 86 30 30 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 824 857 1512 Size: 824 33 655 Fragments arranged by size: 824 655 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 421 507 663 1512 Size: 421 86 156 849 Fragments arranged by size: 849 421 156 86 BclI T'GATC_A Cuts at: 0 162 1512 Size: 162 1350 BglI GCCn_nnn'nGGC Cuts at: 0 34 1512 Size: 34 1478 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 577 1397 1512 Size: 577 820 115 Fragments arranged by size: 820 577 115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 896 1512 Size: 896 616 BsaBI GATnn'nnATC Cuts at: 0 1120 1512 Size: 1120 392 BsaHI Gr'CG_yC Cuts at: 0 16 1512 Size: 16 1496 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 61 1512 Size: 61 1451 BseYI C'CCAG_C Cuts at: 0 489 1512 Size: 489 1023 BsmI GAATG_Cn' Cuts at: 0 1369 1512 Size: 1369 143 BspHI T'CATG_A Cuts at: 0 543 1512 Size: 543 969 BsrFI r'CCGG_y Cuts at: 0 18 1512 Size: 18 1494 BsrGI T'GTAC_A Cuts at: 0 183 1512 Size: 183 1329 BstBI TT'CG_AA Cuts at: 0 1321 1512 Size: 1321 191 BstYI r'GATC_y Cuts at: 0 777 1183 1512 Size: 777 406 329 Fragments arranged by size: 777 406 329 BtgI C'CryG_G Cuts at: 0 725 1125 1512 Size: 725 400 387 Fragments arranged by size: 725 400 387 DraI TTT'AAA Cuts at: 0 363 1016 1512 Size: 363 653 496 Fragments arranged by size: 653 496 363 EaeI y'GGCC_r Cuts at: 0 1280 1512 Size: 1280 232 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 468 1512 Size: 468 1044 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 577 1397 1512 Size: 415 162 820 115 Fragments arranged by size: 820 415 162 115 EcoO109I rG'GnC_Cy Cuts at: 0 71 1512 Size: 71 1441 EcoRI G'AATT_C Cuts at: 0 1482 1512 Size: 1482 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 88 120 1512 Size: 88 32 1392 Fragments arranged by size: 1392 88 32 HincII GTy'rAC Cuts at: 0 371 465 621 1512 Size: 371 94 156 891 Fragments arranged by size: 891 371 156 94 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 787 1236 1512 Size: 787 449 276 Fragments arranged by size: 787 449 276 MscI TGG'CCA Cuts at: 0 1282 1512 Size: 1282 230 MslI CAynn'nnrTG Cuts at: 0 862 1078 1512 Size: 862 216 434 Fragments arranged by size: 862 434 216 MspA1I CmG'CkG Cuts at: 0 489 1512 Size: 489 1023 NaeI GCC'GGC Cuts at: 0 20 1512 Size: 20 1492 NcoI C'CATG_G Cuts at: 0 725 1125 1512 Size: 725 400 387 Fragments arranged by size: 725 400 387 NgoMIV G'CCGG_C Cuts at: 0 18 1512 Size: 18 1494 NspI r_CATG'y Cuts at: 0 774 1493 1512 Size: 774 719 19 Fragments arranged by size: 774 719 19 PfoI T'CCnGG_A Cuts at: 0 554 1512 Size: 554 958 PsiI TTA'TAA Cuts at: 0 658 1334 1512 Size: 658 676 178 Fragments arranged by size: 676 658 178 PvuII CAG'CTG Cuts at: 0 489 1512 Size: 489 1023 SfcI C'TryA_G Cuts at: 0 498 1364 1512 Size: 498 866 148 Fragments arranged by size: 866 498 148 SgrAI Cr'CCGG_yG Cuts at: 0 18 1512 Size: 18 1494 SmlI C'TyrA_G Cuts at: 0 911 1512 Size: 911 601 SspI AAT'ATT Cuts at: 0 216 924 1359 1512 Size: 216 708 435 153 Fragments arranged by size: 708 435 216 153 StyI C'CwwG_G Cuts at: 0 418 725 1125 1512 Size: 418 307 400 387 Fragments arranged by size: 418 400 387 307 TatI w'GTAC_w Cuts at: 0 183 699 1512 Size: 183 516 813 Fragments arranged by size: 813 516 183 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 723 1512 Size: 723 789 XmnI GAAnn'nnTTC Cuts at: 0 303 1512 Size: 303 1209 Enzymes that do cut: AclI AcuI ApoI BaeI BbsI BclI BglI BpmI BpuEI BsaBI BsaHI BseRI BseYI BsmI BspHI BsrFI BsrGI BstBI BstYI BtgI DraI EaeI EciI Eco57MI EcoO109I EcoRI Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PfoI PsiI PvuII SfcI SgrAI SmlI SspI StyI TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI