(Linear) (Six-base) MAPSORT of: KIEE9562.seq Check: 3614 from: 1 to: 1086 KIEE9562.orf With 182 enzymes: SgfI * December 27, 2011 16:42 .. AatII G_ACGT'C Cuts at: 0 164 1086 Size: 164 922 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 667 745 1086 Size: 667 78 341 Fragments arranged by size: 667 341 78 AfeI AGC'GCT Cuts at: 0 125 1086 Size: 125 961 AleI CACnn'nnGTG Cuts at: 0 154 505 1086 Size: 154 351 581 Fragments arranged by size: 581 351 154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 688 1086 Size: 688 398 ApaI G_GGCC'C Cuts at: 0 321 1086 Size: 321 765 AvaI C'yCGr_G Cuts at: 0 57 1086 Size: 57 1029 BamHI G'GATC_C Cuts at: 0 962 1086 Size: 962 124 BanII G_rGCy'C Cuts at: 0 321 795 929 1042 1086 Size: 321 474 134 113 44 Fragments arranged by size: 474 321 134 113 44 BbsI GAAGACnn'nnnn_ Cuts at: 0 375 1086 Size: 375 711 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 795 829 1086 Size: 795 34 257 Fragments arranged by size: 795 257 34 Bme1580I G_kGCm'C Cuts at: 0 321 1086 Size: 321 765 BmrI ACTGGGnnnn_n' Cuts at: 0 588 1086 Size: 588 498 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 30 1061 1086 Size: 30 1031 25 Fragments arranged by size: 1031 30 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 104 562 1086 Size: 75 29 458 524 Fragments arranged by size: 524 458 75 29 Bpu10I CC'TnA_GC Cuts at: 0 196 364 1086 Size: 196 168 722 Fragments arranged by size: 722 196 168 BsaHI Gr'CG_yC Cuts at: 0 161 1086 Size: 161 925 BsaWI w'CCGG_w Cuts at: 0 212 1086 Size: 212 874 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 343 487 619 1086 Size: 343 144 132 467 Fragments arranged by size: 467 343 144 132 BseYI C'CCAG_C Cuts at: 0 314 730 788 1041 1086 Size: 314 416 58 253 45 Fragments arranged by size: 416 314 253 58 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 552 1084 1086 Size: 460 92 532 2 Fragments arranged by size: 532 460 92 2 BsiHKAI G_wGCw'C Cuts at: 0 140 197 335 1086 Size: 140 57 138 751 Fragments arranged by size: 751 140 138 57 BsmI GAATG_Cn' Cuts at: 0 915 995 1086 Size: 915 80 91 Fragments arranged by size: 915 91 80 Bsp1286I G_dGCh'C Cuts at: 0 140 197 321 335 795 929 1042 1086 Size: 140 57 124 14 460 134 113 44 Fragments arranged by size: 460 140 134 124 113 57 44 14 BspEI T'CCGG_A Cuts at: 0 212 1086 Size: 212 874 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 211 668 1086 Size: 211 457 418 Fragments arranged by size: 457 418 211 BsrFI r'CCGG_y Cuts at: 0 455 1086 Size: 455 631 BssHII G'CGCG_C Cuts at: 0 80 1086 Size: 80 1006 BssSI C'ACGA_G Cuts at: 0 292 1086 Size: 292 794 BstAPI GCAn_nnn'nTGC Cuts at: 0 719 1086 Size: 719 367 BstXI CCAn_nnnn'nTGG Cuts at: 0 156 762 1086 Size: 156 606 324 Fragments arranged by size: 606 324 156 BstYI r'GATC_y Cuts at: 0 851 962 1086 Size: 851 111 124 Fragments arranged by size: 851 124 111 Bsu36I CC'TnA_GG Cuts at: 0 857 1086 Size: 857 229 BtgI C'CryG_G Cuts at: 0 131 155 503 985 1086 Size: 131 24 348 482 101 Fragments arranged by size: 482 348 131 101 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 384 1086 Size: 384 702 BtsI GCAGTG_nn' Cuts at: 0 56 333 392 614 990 1086 Size: 56 277 59 222 376 96 Fragments arranged by size: 376 277 222 96 59 56 EaeI y'GGCC_r Cuts at: 0 296 821 1086 Size: 296 525 265 Fragments arranged by size: 525 296 265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 104 562 667 745 1086 Size: 75 29 458 105 78 341 Fragments arranged by size: 458 341 105 78 75 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 325 1086 Size: 325 761 EcoO109I rG'GnC_Cy Cuts at: 0 95 318 536 1086 Size: 95 223 218 550 Fragments arranged by size: 550 223 218 95 FspI TGC'GCA Cuts at: 0 664 1086 Size: 664 422 HaeII r_GCGC'y Cuts at: 0 127 350 462 1086 Size: 127 223 112 624 Fragments arranged by size: 624 223 127 112 MscI TGG'CCA Cuts at: 0 298 823 1086 Size: 298 525 263 Fragments arranged by size: 525 298 263 MslI CAynn'nnrTG Cuts at: 0 154 505 670 760 1060 1086 Size: 154 351 165 90 300 26 Fragments arranged by size: 351 300 165 154 90 26 MspA1I CmG'CkG Cuts at: 0 133 902 1086 Size: 133 769 184 Fragments arranged by size: 769 184 133 PasI CC'CwG_GG Cuts at: 0 110 321 1086 Size: 110 211 765 Fragments arranged by size: 765 211 110 PflMI CCAn_nnn'nTGG Cuts at: 0 435 788 840 1086 Size: 435 353 52 246 Fragments arranged by size: 435 353 246 52 PfoI T'CCnGG_A Cuts at: 0 91 1086 Size: 91 995 PpuMI rG'GwC_Cy Cuts at: 0 95 536 1086 Size: 95 441 550 Fragments arranged by size: 550 441 95 PspOMI G'GGCC_C Cuts at: 0 317 1086 Size: 317 769 PstI C_TGCA'G Cuts at: 0 1037 1086 Size: 1037 49 PvuII CAG'CTG Cuts at: 0 902 1086 Size: 902 184 SacII CC_GC'GG Cuts at: 0 134 1086 Size: 134 952 SanDI GG'GwC_CC Cuts at: 0 536 1086 Size: 536 550 SexAI A'CCwGG_T Cuts at: 0 257 1086 Size: 257 829 SfcI C'TryA_G Cuts at: 0 1033 1086 Size: 1033 53 SmaI CCC'GGG Cuts at: 0 59 1086 Size: 59 1027 SrfI GCCC'GGGC Cuts at: 0 59 1086 Size: 59 1027 StyI C'CwwG_G Cuts at: 0 1010 1086 Size: 1010 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 102 607 660 1065 1086 Size: 102 505 53 405 21 Fragments arranged by size: 505 405 102 53 21 TatI w'GTAC_w Cuts at: 0 596 1086 Size: 596 490 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 528 1086 Size: 528 558 XmaI C'CCGG_G Cuts at: 0 57 1086 Size: 57 1029 ZraI GAC'GTC Cuts at: 0 162 1086 Size: 162 924 Enzymes that do cut: AatII AcuI AfeI AleI AlfI ApaI AvaI BamHI BanII BbsI BcgI Bme1580I BmrI BplI BpmI Bpu10I BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI Eco57MI EcoNI EcoO109I FspI HaeII MscI MslI MspA1I PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SanDI SexAI SfcI SmaI SrfI StyI TaqII TatI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AflIII AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaXI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrGI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI