################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MaeII MboI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AciI 1123 GCGG CCGC(-3/-1) AfaI 952 GTAC GT^AC AgeI 362 ACCGGT A^CCGGT ApaI 817 GGGCCC GGGCC^C AvaI 1011 CTCGGG C^[CT]CG[GA]G AvaII 1032 GGTCC G^G[AT]CC BbvCI 397 GCTGAGG CCTCAGC(-5/-2) BglI 649 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 345 CTCCAG CTGGAG(16/14) BsaBI 1270 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 1010 CCTCGG C^C[ACGT][ACGT]GG BsaWI 362 ACCGGT [AT]^CCGG[AT] BsmI 160 GAATGC GAATGC(1/-1) BsoBI 1011 CTCGGG C^[CT]CG[GA]G BsrFI 362 ACCGGT [GA]^CCGG[CT] BstEII 75 GGTCACC G^GT[ACGT]ACC BstPI 75 GGTCACC G^GT[ACGT]ACC Cfr10I 362 ACCGGT [GA]^CCGG[CT] DraII 816 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO109I 816 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 75 GGTCACC G^GT[ACGT]ACC EcoRI 3 GAATTC G^AATTC EcoRV 895 GATATC GAT^ATC FauI 1123 GCGGG CCCGC(4/6) HincII 171 GTCAAC GT[CT]^[GA]AC HindII 171 GTCAAC GT[CT]^[GA]AC MamI 1270 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PinAI 362 ACCGGT A^CCGGT RsaI 952 GTAC GT^AC SspI 1019 AATATT AAT^ATT TaqI 630 TCGA T^CGA TthHB8I 630 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 374 GACACCGTC BcnI 72 CCCGG CC^[GC]GG 408 CCGGG BfaI 526 CTAG C^TAG 974 CTAG BsgI 778 CTGCAC GTGCAG(16/14) 812 GTGCAG BspHI 570 TCATGA T^CATGA 876 TCATGA BstNI 1049 CCTGG CC^[AT]GG 1236 CCTGG EcoRII 1049 CCTGG ^CC[AT]GG 1236 CCTGG HindIII 415 AAGCTT A^AGCTT 449 AAGCTT MaeI 526 CTAG C^TAG 974 CTAG MaeIII 76 GTCAC ^GT[ACGT]AC 938 GTGAC MslI 757 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 943 CATCAAGATG MvaI 1049 CCTGG CC^[AT]GG 1236 CCTGG NciI 72 CCCGG CC^[GC]GG 408 CCGGG NlaIV 323 GGAGCC GG[ACGT]^[ACGT]CC 817 GGGCCC NspI 782 ACATGC [GA]CATG^[CT] 923 GCATGT PflFI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 374 GACACCGTC PflMI 383 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 494 CCAGATGCTGG RcaI 570 TCATGA T^CATGA 876 TCATGA SacI 258 GAGCTC GAGCT^C 805 GAGCTC StuI 106 AGGCCT AGG^CCT 1330 AGGCCT Tsp45I 76 GTCAC ^GT[GC]AC 938 GTGAC Tth111I 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 374 GACACCGTC Van91I 383 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 494 CCAGATGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AcsI AluI AlwNI ApoI Asp700I AspHI BanII BbvI BmyI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I Cac8I Cfr13I DdeI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboII MnlI MseI MspI MwoI NlaIII PleI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI XmnI