################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HpaI KpnI Ksp632I KspI MaeIII MamI MboII MfeI MluI MroI MspA1I MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacII SalI SapI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 1018 GTAGAC GT^[AC][GT]AC AccII 675 CGCG CG^CG AflIII 480 ACACGT A^C[GA][CT]GT AhdI 456 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 267 GGATC GGATC(4/5) AocI 883 CCTGAGG CC^T[ACGT]AGG AspEI 456 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 834 GAGCTC G[AT]GC[AT]^C BalI 707 TGGCCA TGG^CCA BbrPI 481 CACGTG CAC^GTG BfaI 649 CTAG C^TAG BglI 347 GCCACAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 481 CACGTG [CT]AC^GT[GA] BseRI 895 CTCCTC GAGGAG(10/8) BsgI 1009 CTGCAC GTGCAG(16/14) BsiHKAI 834 GAGCTC G[AT]GC[AT]^C BstUI 675 CGCG CG^CG BstYI 267 GGATCT [GA]^GATC[CT] Bsu36I 883 CCTGAGG CC^T[ACGT]AGG DpnII 268 GATC ^GATC DsaI 4 CCGTGG C^C[GA][CT]GG Eam1105I 456 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 883 CCTGAGG CC^T[ACGT]AGG EcoNI 879 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 998 CCAAGG C^C[AT][AT]GG FseI 534 GGCCGGCC GGCCGG^CC HindIII 796 AAGCTT A^AGCTT MaeI 649 CTAG C^TAG MboI 268 GATC ^GATC MflI 267 GGATCT [GA]^GATC[CT] MluNI 707 TGGCCA TGG^CCA MscI 707 TGGCCA TGG^CCA MseI 800 TTAA T^TAA MslI 153 CATGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 675 CGCG CG^CG NdeII 268 GATC ^GATC PmaCI 481 CACGTG CAC^GTG PmlI 481 CACGTG CAC^GTG PpuMI 770 AGGTCCT [GA]G^G[AT]CC[CT] SacI 834 GAGCTC GAGCT^C Sau3AI 268 GATC ^GATC SexAI 869 ACCTGGT A^CC[AT]GGT SfaNI 293 GCATC GCATC(5/9) StyI 998 CCAAGG C^C[AT][AT]GG TaqI 101 TCGA T^CGA TfiI 1030 GAATC G^A[AT]TC Tru9I 800 TTAA T^TAA TthHB8I 101 TCGA T^CGA XcmI 992 CCAGCCCCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 267 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 735 GGCGCC G[GA]^CG[CT]C 950 GGCGCC BbeI 735 GGCGCC GGCGC^C 950 GGCGCC BbvCI 342 CCTCAGC CCTCAGC(-5/-2) 814 GCTGAGG BsaHI 735 GGCGCC G[GA]^CG[CT]C 950 GGCGCC BsaI 85 GAGACC GGTCTC(1/5) 435 GGTCTC BspMI 281 ACCTGC ACCTGC(4/8) 768 GCAGGT BsrI 803 ACTGG ACTGG(1/-1) 740 CCAGT BssSI 528 CACGAG CACGAG(-5/-1) 514 CTCGTG BstXI 164 CCATCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 200 CCAACCGGCTGG HgaI 456 GACGC GACGC(5/10) 920 GCGTC Hin1I 735 GGCGCC G[GA]^CG[CT]C 950 GGCGCC KasI 735 GGCGCC G^GCGCC 950 GGCGCC NaeI 535 GCCGGC GCC^GGC 947 GCCGGC NarI 735 GGCGCC GG^CGCC 950 GGCGCC NgoMIV 535 GCCGGC G^CCGGC 947 GCCGGC SbfI 765 CCTGCAGG CCTGCA^GG 840 CCTGCAGG SfoI 735 GGCGCC GGC^GCC 950 GGCGCC SmaI 64 CCCGGG CCC^GGG 1025 CCCGGG Sse8387I 765 CCTGCAGG CCTGCA^GG 840 CCTGCAGG XmaCI 64 CCCGGG C^CCGGG 1025 CCCGGG XmaI 64 CCCGGG C^CCGGG 1025 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AluI AlwNI ApaI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PleI PstI Sau96I ScrFI SfcI StuI TseI TspRI