(Circular) (Six-base) MAPSORT of: pFN21AE1135.seq Check: 7506 from: 1 to: 5764 pFN21AE1135 5764 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3024 3107 1937 Size: 1087 83 4594 Fragments arranged by size: 4594 1087 83 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2098 3827 5211 5479 1632 Size: 466 1729 1384 268 1917 Fragments arranged by size: 1917 1729 1384 466 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 2485 4663 5640 1203 Size: 1282 2178 977 1327 Fragments arranged by size: 2178 1327 1282 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 2466 4493 2466 Size: 2027 3737 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5764 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AlwNI CAG_nnn'CTG Cuts at: 2450 2569 2779 2819 2854 3507 5079 2450 Size: 119 210 40 35 653 1572 3135 Fragments arranged by size: 3135 1572 653 210 119 40 35 ApaI G_GGCC'C Cuts at: 2047 2150 3053 2047 Size: 103 903 4758 Fragments arranged by size: 4758 903 103 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 3073 3299 3451 5560 1493 Size: 1580 226 152 2109 1697 Fragments arranged by size: 2109 1697 1580 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2124 3030 3089 1870 Size: 73 126 55 906 59 4545 Fragments arranged by size: 4545 906 126 73 59 55 BamHI G'GATC_C Cuts at: 3094 3461 3094 Size: 367 5397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2164 2672 2740 2867 Size: 325 217 627 286 91 508 68 127 Cuts at: 2867 2878 2949 2955 2972 3085 4540 618 Size: 11 71 6 17 113 1455 1842 Fragments arranged by size: 1842 1455 627 508 325 286 217 127 113 91 71 68 17 11 6 BanII G_rGCy'C Cuts at: 1391 2047 2150 2843 3053 3083 1391 Size: 656 103 693 210 30 4072 Fragments arranged by size: 4072 693 656 210 103 30 BbeI G_GCGC'C Cuts at: 2744 2959 2744 Size: 215 5549 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4793 BbvCI CC'TCA_GC Cuts at: 2348 2820 2348 Size: 472 5292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3955 3989 1903 Size: 34 50 34 1934 34 3678 Fragments arranged by size: 3678 1934 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2358 4375 136 Size: 107 122 71 631 605 686 2017 1525 Fragments arranged by size: 2017 1525 686 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 2047 2150 2169 2482 2526 2604 2870 2883 Size: 257 103 19 313 44 78 266 13 Cuts at: 2883 2975 3053 3825 4981 1790 Size: 92 78 772 1156 2573 Fragments arranged by size: 2573 1156 772 313 266 257 103 92 78 78 44 19 13 BmgBI CAC'GTC Cuts at: 1318 2410 1318 Size: 1092 4672 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2739 2818 3495 Size: 416 53 83 399 24 1322 79 677 Cuts at: 3495 4453 442 Size: 958 1753 Fragments arranged by size: 1753 1322 958 677 416 399 83 79 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2354 2980 4424 1239 Size: 995 120 626 1444 2579 Fragments arranged by size: 2579 1444 995 626 120 Bpu10I CC'TnA_GC Cuts at: 2348 2402 2417 2633 2733 2820 2837 2348 Size: 54 15 216 100 87 17 5275 Fragments arranged by size: 5275 216 100 87 54 17 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3889 4754 5052 3889 Size: 865 298 4601 Fragments arranged by size: 4601 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 2446 4427 882 Size: 617 585 362 1981 2219 Fragments arranged by size: 2219 1981 617 585 362 BsaAI yAC'GTr Cuts at: 493 1204 2488 3555 493 Size: 711 1284 1067 2702 Fragments arranged by size: 2702 1284 1067 711 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2741 2956 3571 3953 Size: 53 83 186 936 1208 215 615 382 Cuts at: 3953 4588 275 Size: 635 1451 Fragments arranged by size: 1451 1208 936 635 615 382 215 186 83 53 BsaWI w'CCGG_w Cuts at: 4197 4595 4869 5016 5427 4197 Size: 398 274 147 411 4534 Fragments arranged by size: 4534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2186 2216 2808 2838 1178 Size: 30 165 30 783 30 592 30 4104 Fragments arranged by size: 4104 783 592 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2891 1186 Size: 1705 4059 BseYI C'CCAG_C Cuts at: 2686 4967 5463 2686 Size: 2281 496 2987 Fragments arranged by size: 2987 2281 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2999 1163 Size: 573 1263 3928 Fragments arranged by size: 3928 1263 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3214 3975 5003 1540 Size: 275 186 1213 761 1028 2301 Fragments arranged by size: 2301 1213 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2843 3825 3910 4981 2843 Size: 982 85 1071 3626 Fragments arranged by size: 3626 1071 982 85 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2150 2169 2482 2526 2604 2843 Size: 399 257 103 19 313 44 78 239 Cuts at: 2843 2870 2883 2975 3053 3083 3825 3910 4981 Size: 27 13 92 78 30 742 85 1071 Cuts at: 4981 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 313 257 239 103 92 85 78 78 44 30 27 19 13 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2295 2764 3119 844 Size: 1451 469 355 3489 Fragments arranged by size: 3489 1451 469 355 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4259 4433 65 Size: 1183 3011 174 1396 Fragments arranged by size: 3011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2161 2208 2540 2586 2952 4408 4595 Size: 110 351 47 332 46 366 1456 187 Cuts at: 4595 1700 Size: 2869 Fragments arranged by size: 2869 1456 366 351 332 187 110 47 46 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 2519 2533 3824 4836 2519 Size: 14 1291 1012 3447 Fragments arranged by size: 3447 1291 1012 14 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5764 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5764 BstXI CCAn_nnnn'nTGG Cuts at: 2176 2212 3061 2176 Size: 36 849 4879 Fragments arranged by size: 4879 849 36 BstYI r'GATC_y Cuts at: 1972 2272 3094 3461 3846 3863 5315 5401 5413 Size: 300 822 367 385 17 1452 86 12 Cuts at: 5413 5759 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 822 385 367 346 300 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2889 2889 Size: 5764 BtgI C'CryG_G Cuts at: 513 1065 2009 513 Size: 552 944 4268 Fragments arranged by size: 4268 944 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 2307 2915 3016 3280 4074 4094 2307 Size: 608 101 264 794 20 3977 Fragments arranged by size: 3977 794 608 264 101 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2057 2712 Size: 54 1425 50 275 18 201 26 655 Cuts at: 2712 3211 4100 5729 8 Size: 499 889 1629 43 Fragments arranged by size: 1629 1425 889 655 499 275 201 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3694 1353 Size: 2341 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2841 2841 Size: 5764 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2234 2354 2980 3827 4424 5211 Size: 393 466 136 120 626 847 597 787 Cuts at: 5211 5479 1239 Size: 268 1524 Fragments arranged by size: 1524 847 787 626 597 466 393 268 136 120 EcoNI CCTnn'n_nnAGG Cuts at: 2888 2888 Size: 5764 EcoO109I rG'GnC_Cy Cuts at: 2043 2147 2776 3049 3050 2043 Size: 104 629 273 1 4757 Fragments arranged by size: 4757 629 273 104 1 EcoRI G'AATT_C Cuts at: 3073 3451 3073 Size: 378 5386 FseI GG_CCGG'CC Cuts at: 2544 2544 Size: 5764 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 2744 2897 2959 4911 1678 Size: 1066 153 62 1952 2531 Fragments arranged by size: 2531 1952 1066 153 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2452 2484 4405 Size: 32 135 32 614 32 234 32 1921 Cuts at: 4405 4437 4479 4511 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1921 614 234 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 2801 748 Size: 2053 3711 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KasI G'GCGC_C Cuts at: 2740 2955 2740 Size: 215 5549 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2229 4878 5062 1305 Size: 924 2649 184 2007 Fragments arranged by size: 2649 2007 924 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2714 10 Size: 54 1425 343 882 3060 Fragments arranged by size: 3060 1425 882 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 3722 4081 4240 518 Size: 648 996 1560 359 159 2042 Fragments arranged by size: 2042 1560 996 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3180 3857 5005 5250 1606 Size: 1574 677 1148 245 2120 Fragments arranged by size: 2120 1574 1148 677 245 NaeI GCC'GGC Cuts at: 2542 2954 2542 Size: 412 5352 NarI GG'CG_CC Cuts at: 2741 2956 2741 Size: 215 5549 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5212 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NgoMIV G'CCGG_C Cuts at: 2540 2952 2540 Size: 412 5352 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 3122 4667 1132 Size: 97 1893 1545 2229 Fragments arranged by size: 2229 1893 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4862 PfoI T'CCnGG_A Cuts at: 2433 2778 2433 Size: 345 5419 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PmlI CAC'GTG Cuts at: 2488 2488 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2776 2776 Size: 5764 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PspOMI G'GGCC_C Cuts at: 2043 2146 3049 2043 Size: 103 903 4758 Fragments arranged by size: 4758 903 103 PstI C_TGCA'G Cuts at: 830 2724 2775 2850 2922 3116 830 Size: 1894 51 75 72 194 3478 Fragments arranged by size: 3478 1894 194 75 72 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 SacI G_AGCT'C Cuts at: 2843 2843 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SbfI CC_TGCA'GG Cuts at: 2775 2850 3116 2775 Size: 75 266 5423 Fragments arranged by size: 5423 266 75 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SexAI A'CCwGG_T Cuts at: 2874 2874 Size: 5764 SfcI C'TryA_G Cuts at: 826 1045 2720 2771 2846 2918 3112 4247 4928 Size: 219 1675 51 75 72 194 1135 681 Cuts at: 4928 5119 826 Size: 191 1471 Fragments arranged by size: 1675 1471 1135 681 219 194 191 75 72 51 SfoI GGC'GCC Cuts at: 2742 2957 2742 Size: 215 5549 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 2071 3032 3091 1872 Size: 199 961 59 4545 Fragments arranged by size: 4545 961 199 59 SmlI C'TyrA_G Cuts at: 820 1017 1943 3868 4769 5031 820 Size: 197 926 1925 901 262 1553 Fragments arranged by size: 1925 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 2694 2835 2989 1517 Size: 1177 141 154 4292 Fragments arranged by size: 4292 1177 154 141 StyI C'CwwG_G Cuts at: 513 1065 3003 3203 513 Size: 552 1938 200 3074 Fragments arranged by size: 3074 1938 552 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2320 3791 3808 3961 4146 4591 325 Size: 1255 740 1471 17 153 185 445 1498 Fragments arranged by size: 1498 1471 1255 740 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4010 5722 96 Size: 274 80 33 51 1443 2033 1712 138 Fragments arranged by size: 2033 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2808 2840 2933 2965 5724 5756 2808 Size: 32 93 32 2759 32 2816 Fragments arranged by size: 2816 2759 93 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3004 3004 Size: 5764 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 2069 3030 3089 1870 Size: 199 961 59 4545 Fragments arranged by size: 4545 961 199 59 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI