(Linear) (Six-base) MAPSORT of: KIEE1135.seq Check: 9809 from: 1 to: 1059 KIEE1135.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AccI GT'mk_AC Cuts at: 0 1019 1059 Size: 1019 40 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 1059 Size: 93 966 AflIII A'CryG_T Cuts at: 0 480 1059 Size: 480 579 AhdI GACnn_n'nnGTC Cuts at: 0 461 1059 Size: 461 598 AlwNI CAG_nnn'CTG Cuts at: 0 445 564 774 814 849 1059 Size: 445 119 210 40 35 210 Fragments arranged by size: 445 210 210 119 40 35 ApaI G_GGCC'C Cuts at: 0 42 145 1048 1059 Size: 42 103 903 11 Fragments arranged by size: 903 103 42 11 AvaI C'yCGr_G Cuts at: 0 64 119 1025 1059 Size: 64 55 906 34 Fragments arranged by size: 906 64 55 34 BanI G'GyrC_C Cuts at: 0 68 159 667 735 862 873 944 950 Size: 68 91 508 68 127 11 71 6 Cuts at: 950 967 1059 Size: 17 92 Fragments arranged by size: 508 127 92 91 71 68 68 17 11 6 BanII G_rGCy'C Cuts at: 0 42 145 838 1048 1059 Size: 42 103 693 210 11 Fragments arranged by size: 693 210 103 42 11 BbeI G_GCGC'C Cuts at: 0 739 954 1059 Size: 739 215 105 Fragments arranged by size: 739 215 105 BbvCI CC'TCA_GC Cuts at: 0 343 815 1059 Size: 343 472 244 Fragments arranged by size: 472 343 244 BglI GCCn_nnn'nGGC Cuts at: 0 353 1059 Size: 353 706 Bme1580I G_kGCm'C Cuts at: 0 42 145 164 477 521 599 865 878 Size: 42 103 19 313 44 78 266 13 Cuts at: 878 970 1048 1059 Size: 92 78 11 Fragments arranged by size: 313 266 103 92 78 78 44 42 19 13 11 BmgBI CAC'GTC Cuts at: 0 405 1059 Size: 405 654 BmrI ACTGGGnnnn_n' Cuts at: 0 734 813 1059 Size: 734 79 246 Fragments arranged by size: 734 246 79 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 349 975 1059 Size: 229 120 626 84 Fragments arranged by size: 626 229 120 84 Bpu10I CC'TnA_GC Cuts at: 0 343 397 412 628 728 815 832 1059 Size: 343 54 15 216 100 87 17 227 Fragments arranged by size: 343 227 216 100 87 54 17 15 BsaI GGTCTCn'nnnn_ Cuts at: 0 79 441 1059 Size: 79 362 618 Fragments arranged by size: 618 362 79 BsaAI yAC'GTr Cuts at: 0 483 1059 Size: 483 576 BsaHI Gr'CG_yC Cuts at: 0 736 951 1059 Size: 736 215 108 Fragments arranged by size: 736 215 108 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 181 211 803 833 1059 Size: 181 30 592 30 226 Fragments arranged by size: 592 226 181 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 886 1059 Size: 886 173 BseYI C'CCAG_C Cuts at: 0 681 1059 Size: 681 378 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 994 1059 Size: 994 65 BsiHKAI G_wGCw'C Cuts at: 0 838 1059 Size: 838 221 Bsp1286I G_dGCh'C Cuts at: 0 42 145 164 477 521 599 838 865 Size: 42 103 19 313 44 78 239 27 Cuts at: 865 878 970 1048 1059 Size: 13 92 78 11 Fragments arranged by size: 313 239 103 92 78 78 44 42 27 19 13 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 290 759 1059 Size: 290 469 300 Fragments arranged by size: 469 300 290 BsrFI r'CCGG_y Cuts at: 0 156 203 535 581 947 1059 Size: 156 47 332 46 366 112 Fragments arranged by size: 366 332 156 112 47 46 BssSI C'ACGA_G Cuts at: 0 514 528 1059 Size: 514 14 531 Fragments arranged by size: 531 514 14 BstXI CCAn_nnnn'nTGG Cuts at: 0 171 207 1059 Size: 171 36 852 Fragments arranged by size: 852 171 36 BstYI r'GATC_y Cuts at: 0 267 1059 Size: 267 792 Bsu36I CC'TnA_GG Cuts at: 0 884 1059 Size: 884 175 BtgI C'CryG_G Cuts at: 0 4 1059 Size: 4 1055 BtsI GCAGTG_nn' Cuts at: 0 302 910 1011 1059 Size: 302 608 101 48 Fragments arranged by size: 608 302 101 48 EaeI y'GGCC_r Cuts at: 0 26 52 707 1059 Size: 26 26 655 352 Fragments arranged by size: 655 352 26 26 EcoICRI GAG'CTC Cuts at: 0 836 1059 Size: 836 223 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 229 349 975 1059 Size: 93 136 120 626 84 Fragments arranged by size: 626 136 120 93 84 EcoNI CCTnn'n_nnAGG Cuts at: 0 883 1059 Size: 883 176 EcoO109I rG'GnC_Cy Cuts at: 0 38 142 771 1044 1045 1059 Size: 38 104 629 273 1 14 Fragments arranged by size: 629 273 104 38 14 1 FseI GG_CCGG'CC Cuts at: 0 539 1059 Size: 539 520 HaeII r_GCGC'y Cuts at: 0 739 892 954 1059 Size: 739 153 62 105 Fragments arranged by size: 739 153 105 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 181 213 447 479 1059 Size: 181 32 234 32 580 Fragments arranged by size: 580 234 181 32 32 HindIII A'AGCT_T Cuts at: 0 796 1059 Size: 796 263 KasI G'GCGC_C Cuts at: 0 735 950 1059 Size: 735 215 109 Fragments arranged by size: 735 215 109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 224 1059 Size: 224 835 MscI TGG'CCA Cuts at: 0 709 1059 Size: 709 350 MslI CAynn'nnrTG Cuts at: 0 157 1059 Size: 157 902 NaeI GCC'GGC Cuts at: 0 537 949 1059 Size: 537 412 110 Fragments arranged by size: 537 412 110 NarI GG'CG_CC Cuts at: 0 736 951 1059 Size: 736 215 108 Fragments arranged by size: 736 215 108 NgoMIV G'CCGG_C Cuts at: 0 535 947 1059 Size: 535 412 112 Fragments arranged by size: 535 412 112 PfoI T'CCnGG_A Cuts at: 0 428 773 1059 Size: 428 345 286 Fragments arranged by size: 428 345 286 PmlI CAC'GTG Cuts at: 0 483 1059 Size: 483 576 PpuMI rG'GwC_Cy Cuts at: 0 771 1059 Size: 771 288 PspOMI G'GGCC_C Cuts at: 0 38 141 1044 1059 Size: 38 103 903 15 Fragments arranged by size: 903 103 38 15 PstI C_TGCA'G Cuts at: 0 719 770 845 917 1059 Size: 719 51 75 72 142 Fragments arranged by size: 719 142 75 72 51 SacI G_AGCT'C Cuts at: 0 838 1059 Size: 838 221 SbfI CC_TGCA'GG Cuts at: 0 770 845 1059 Size: 770 75 214 Fragments arranged by size: 770 214 75 SexAI A'CCwGG_T Cuts at: 0 869 1059 Size: 869 190 SfcI C'TryA_G Cuts at: 0 715 766 841 913 1059 Size: 715 51 75 72 146 Fragments arranged by size: 715 146 75 72 51 SfoI GGC'GCC Cuts at: 0 737 952 1059 Size: 737 215 107 Fragments arranged by size: 737 215 107 SmaI CCC'GGG Cuts at: 0 66 1027 1059 Size: 66 961 32 Fragments arranged by size: 961 66 32 StuI AGG'CCT Cuts at: 0 689 830 984 1059 Size: 689 141 154 75 Fragments arranged by size: 689 154 141 75 StyI C'CwwG_G Cuts at: 0 998 1059 Size: 998 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 315 1059 Size: 315 744 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 803 835 928 960 1059 Size: 803 32 93 32 99 Fragments arranged by size: 803 99 93 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 999 1059 Size: 999 60 XmaI C'CCGG_G Cuts at: 0 64 1025 1059 Size: 64 961 34 Fragments arranged by size: 961 64 34 Enzymes that do cut: AccI AcuI AflIII AhdI AlwNI ApaI AvaI BanI BanII BbeI BbvCI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstXI BstYI Bsu36I BtgI BtsI EaeI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HindIII KasI MmeI MscI MslI NaeI NarI NgoMIV PfoI PmlI PpuMI PspOMI PstI SacI SbfI SexAI SfcI SfoI SmaI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoRI EcoRV FalI FspI FspAI HincII HpaI KpnI MfeI MluI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI