Restriction map for
pFN21AB5770
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI DraI DraIII Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT14I FseI
FspI HaeII Hin1I HindIII KasI
MamI MluI MroI MslI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 1821 GGTACC G^GTACC
AccI 290 GTAGAC GT^[AC][GT]AC
Asp700I 637 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1821 GGTACC G^GTACC
BalI 978 TGGCCA TGG^CCA
BanI 1821 GGTACC G^G[CT][GA]CC
BanII 1673 GAGCTC G[GA]GC[CT]^C
BbsI 1519 GTCTTC GAAGAC(2/6)
BbvI 16 GCTGC GCAGC(8/12)
BclI 144 TGATCA T^GATCA
BglII 719 AGATCT A^GATCT
BlpI 1918 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1918 GCTGAGC GC^T[ACGT]AGC
BpuAI 1519 GTCTTC GAAGAC(2/6)
BsiEI 1529 CGGCCG CG[GA][CT]^CG
BsmI 1554 GCATTC GAATGC(1/-1)
BspHI 1593 TCATGA T^CATGA
BspLU11I 1877 ACATGT A^CATGT
BspMI 2000 ACCTGC ACCTGC(4/8)
BsrBI 2083 GAGCGG CCGCTC(-3/-3)
BsrFI 1531 GCCGGT [GA]^CCGG[CT]
BsrI 266 CCAGT ACTGG(1/-1)
BstF5I 549 GGATG GGATG(2/0)
BstXI 1616 CCACAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 719 AGATCT [GA]^GATC[CT]
CelII 1918 GCTGAGC GC^T[ACGT]AGC
CfoI 14 GCGC GCG^C
Cfr10I 1531 GCCGGT [GA]^CCGG[CT]
DrdI 2099 GACGAAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 73 CCGCGG C^C[GA][CT]GG
EagI 1529 CGGCCG C^GGCCG
EclXI 1529 CGGCCG C^GGCCG
Eco52I 1529 CGGCCG C^GGCCG
EcoT22I 2033 ATGCAT ATGCA^T
FbaI 144 TGATCA T^GATCA
FokI 549 GGATG GGATG(9/13)
HapII 1532 CCGG C^CGG
HgaI 2106 GCGTC GACGC(5/10)
HhaI 14 GCGC GCG^C
HinP1I 14 GCGC G^CGC
HincII 673 GTTAAC GT[CT]^[GA]AC
HindII 673 GTTAAC GT[CT]^[GA]AC
HpaI 673 GTTAAC GTT^AAC
HpaII 1532 CCGG C^CGG
KpnI 1821 GGTACC GGTAC^C
KspI 73 CCGCGG CCGC^GG
MfeI 1148 CAATTG C^AATTG
MflI 719 AGATCT [GA]^GATC[CT]
MluNI 978 TGGCCA TGG^CCA
MscI 978 TGGCCA TGG^CCA
MspI 1532 CCGG C^CGG
MunI 1148 CAATTG C^AATTG
MwoI 1390 GCTGGCCGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 2033 ATGCAT ATGCA^T
NspI 1877 ACATGT [GA]CATG^[CT]
PleI 766 GACTC GAGTC(4/5)
PvuII 1916 CAGCTG CAG^CTG
RcaI 1593 TCATGA T^CATGA
SacI 1673 GAGCTC GAGCT^C
SacII 73 CCGCGG CCGC^GG
ScaI 1548 AGTACT AGT^ACT
SpeI 208 ACTAGT A^CTAGT
TseI 16 GCTGC G^C[AT]GC
XbaI 1033 TCTAGA T^CTAGA
XhoII 719 AGATCT [GA]^GATC[CT]
XmnI 637 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 32 CGCG CG^CG
74 CGCG
AflIII 677 ACACGT A^C[GA][CT]GT
1877 ACATGT
AhdI 1906 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2140 GACCAAAGGTC
AlwNI 604 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1112 CAGTACCTG
AspEI 1906 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2140 GACCAAAGGTC
AvaII 78 GGACC G^G[AT]CC
2046 GGTCC
BsaI 187 GAGACC GGTCTC(1/5)
580 GGTCTC
BsgI 27 CTGCAC GTGCAG(16/14)
1415 GTGCAG
BspDI 164 ATCGAT AT^CGAT
1175 ATCGAT
BsrDI 2036 CATTGC GCAATG(2/0)
404 GCAATG
BstUI 32 CGCG CG^CG
74 CGCG
Cfr13I 78 GGACC G^G[ACGT]CC
2046 GGTCC
ClaI 164 ATCGAT AT^CGAT
1175 ATCGAT
DraII 77 GGGACCC [GA]G^G[ACGT]CC[CT]
2045 AGGTCCT
Eam1105I 1906 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2140 GACCAAAGGTC
EcoNI 59 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
864 CCTAAGAGAGG
EcoO109I 77 GGGACCC [GA]G^G[ACGT]CC[CT]
2045 AGGTCCT
FauI 72 CCCGC CCCGC(4/6)
75 GCGGG
Fnu4HI 16 GCTGC GC^[ACGT]GC
1528 GCGGC
ItaI 16 GCTGC GC^[ACGT]GC
1528 GCGGC
MspA1I 73 CCGCGG C[AC]G^C[GT]G
1916 CAGCTG
MvnI 32 CGCG CG^CG
74 CGCG
PpuMI 77 GGGACCC [GA]G^G[AT]CC[CT]
2045 AGGTCCT
PstI 1410 CTGCAG CTGCA^G
1544 CTGCAG
Sau96I 78 GGACC G^G[ACGT]CC
2046 GGTCC
Tsp45I 1358 GTGAC ^GT[GC]AC
1889 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstNI Cac8I DdeI
DpnII EaeI EarI EcoRII HaeIII
HinfI HphI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV RsaI
SapI Sau3AI ScrFI SfaNI SfcI
SspI TaqI TfiI Tru9I Tsp509I
TspRI TthHB8I