(Circular) (Six-base) MAPSORT of: pFN21AB5770.seq Check: 3222 from: 1 to: 6988 pFN21AB5770 6988 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4014 4014 Size: 6988 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4798 278 Size: 53 83 186 936 3262 2468 Fragments arranged by size: 3262 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2296 4331 1937 Size: 359 2035 4594 Fragments arranged by size: 4594 2035 359 Acc65I G'GTAC_C Cuts at: 3826 4309 3826 Size: 483 6505 AclI AA'CG_TT Cuts at: 1563 5115 5488 1563 Size: 3552 373 3063 Fragments arranged by size: 3552 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2053 3281 4067 5051 6435 6703 1632 Size: 421 1228 786 984 1384 268 1917 Fragments arranged by size: 1917 1384 1228 984 786 421 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6791 AflIII A'CryG_T Cuts at: 1203 2682 3882 5887 6864 1203 Size: 1479 1200 2005 977 1327 Fragments arranged by size: 2005 1479 1327 1200 977 AgeI A'CCGG_T Cuts at: 5819 5819 Size: 6988 AhdI GACnn_n'nnGTC Cuts at: 3916 4150 5717 3916 Size: 234 1567 5187 Fragments arranged by size: 5187 1567 234 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6988 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6988 AlwNI CAG_nnn'CTG Cuts at: 2614 3122 4731 6303 2614 Size: 508 1609 1572 3299 Fragments arranged by size: 3299 1609 1572 508 ApaLI G'TGCA_C Cuts at: 5045 6201 5045 Size: 1156 5832 ApoI r'AATT_y Cuts at: 1493 2632 2879 2916 2930 3309 3602 3625 3668 Size: 1139 247 37 14 379 293 23 43 Cuts at: 3668 3851 4180 4297 4523 4675 6784 1493 Size: 183 329 117 226 152 2109 1697 Fragments arranged by size: 2109 1697 1139 379 329 293 247 226 183 152 117 43 37 23 14 AseI AT'TA_AT Cuts at: 160 1032 5542 160 Size: 872 4510 1606 Fragments arranged by size: 4510 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6988 AvaI C'yCGr_G Cuts at: 1870 1943 4313 1870 Size: 73 2370 4545 Fragments arranged by size: 4545 2370 73 BamHI G'GATC_C Cuts at: 4318 4685 4318 Size: 367 6621 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3826 4309 5764 618 Size: 325 217 627 2039 483 1455 1842 Fragments arranged by size: 2039 1842 1455 627 483 325 217 BanII G_rGCy'C Cuts at: 1391 3682 4307 1391 Size: 2291 625 4072 Fragments arranged by size: 4072 2291 625 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3517 928 Size: 971 1618 4399 Fragments arranged by size: 4399 1618 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5179 5213 1903 Size: 34 50 34 3158 34 3678 Fragments arranged by size: 3678 3158 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4880 6090 4880 Size: 1210 5778 BclI T'GATC_A Cuts at: 1548 2149 1548 Size: 601 6387 BfrBI ATG'CAT Cuts at: 4040 4040 Size: 6988 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5599 136 Size: 107 122 71 631 605 3927 1525 Fragments arranged by size: 3927 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2724 6983 2724 Size: 4259 2729 BlpI GC'TnA_GC Cuts at: 3924 4405 3924 Size: 481 6507 Bme1580I G_kGCm'C Cuts at: 1790 5049 6205 1790 Size: 3259 1156 2573 Fragments arranged by size: 3259 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6988 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4719 5677 442 Size: 416 53 83 399 24 3302 958 1753 Fragments arranged by size: 3302 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6988 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2580 2639 3488 5648 1239 Size: 1341 59 849 2160 2579 Fragments arranged by size: 2579 2160 1341 849 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5113 5978 6276 5113 Size: 865 298 5825 Fragments arranged by size: 5825 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2186 2591 5651 882 Size: 617 687 405 3060 2219 Fragments arranged by size: 3060 2219 687 617 405 BsaAI yAC'GTr Cuts at: 493 1204 4779 493 Size: 711 3575 2702 Fragments arranged by size: 3575 2702 711 BsaBI GATnn'nnATC Cuts at: 6982 6982 Size: 6988 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4795 5177 5812 275 Size: 53 83 186 936 3262 382 635 1451 Fragments arranged by size: 3262 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5421 5819 6093 6240 6651 5421 Size: 398 274 147 411 5758 Fragments arranged by size: 5758 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3123 3153 1178 Size: 30 165 30 1720 30 5013 Fragments arranged by size: 5013 1720 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6988 BseYI C'CCAG_C Cuts at: 2423 6191 6687 2423 Size: 3768 496 2724 Fragments arranged by size: 3768 2724 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2017 3441 1163 Size: 573 281 1424 4710 Fragments arranged by size: 4710 1424 573 281 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3537 4438 5199 6227 1540 Size: 275 186 1536 901 761 1028 2301 Fragments arranged by size: 2301 1536 1028 901 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2048 2829 3682 3759 5049 5134 6205 2048 Size: 781 853 77 1290 85 1071 2831 Fragments arranged by size: 2831 1290 1071 853 781 85 77 BsmI GAATG_Cn' Cuts at: 3559 4499 4592 3559 Size: 940 93 5955 Fragments arranged by size: 5955 940 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2829 3682 3759 4307 5049 5134 Size: 399 258 781 853 77 548 742 85 Cuts at: 5134 6205 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 853 781 742 548 399 258 85 77 BspEI T'CCGG_A Cuts at: 6651 6651 Size: 6988 BspHI T'CATG_A Cuts at: 3598 4877 6607 3598 Size: 1279 1730 3979 Fragments arranged by size: 3979 1730 1279 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4014 4343 844 Size: 3170 329 3489 Fragments arranged by size: 3489 3170 329 BsrBI CCG'CTC Cuts at: 1394 1823 4090 4875 1394 Size: 429 2267 785 3507 Fragments arranged by size: 3507 2267 785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2416 4040 5483 5657 65 Size: 1183 1168 1624 1443 174 1396 Fragments arranged by size: 1624 1443 1396 1183 1168 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3536 5632 5819 1700 Size: 110 1726 2096 187 2869 Fragments arranged by size: 2869 2096 1726 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6988 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6988 BssSI C'ACGA_G Cuts at: 5048 6060 5048 Size: 1012 5976 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6988 BstBI TT'CG_AA Cuts at: 5792 5792 Size: 6988 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6988 BstXI CCAn_nnnn'nTGG Cuts at: 3628 3628 Size: 6988 BstYI r'GATC_y Cuts at: 1972 2724 4318 4685 5070 5087 6539 6625 6637 Size: 752 1594 367 385 17 1452 86 12 Cuts at: 6637 6983 1972 Size: 346 1977 Fragments arranged by size: 1977 1594 1452 752 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2078 513 Size: 552 1013 5423 Fragments arranged by size: 5423 1013 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6249 Fragments arranged by size: 6249 683 56 BtsI GCAGTG_nn' Cuts at: 2612 3424 3429 4504 5298 5318 2612 Size: 812 5 1075 794 20 4282 Fragments arranged by size: 4282 1075 812 794 20 5 ClaI AT'CG_AT Cuts at: 1554 2170 3181 1554 Size: 616 1011 5361 Fragments arranged by size: 5361 1011 616 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6953 DraI TTT'AAA Cuts at: 4292 4292 Size: 6988 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4110 5995 809 Size: 507 2794 1885 1802 Fragments arranged by size: 2794 1885 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2983 3397 3534 Size: 54 1425 50 275 18 1153 414 137 Cuts at: 3534 4435 5324 6953 8 Size: 901 889 1629 43 Fragments arranged by size: 1629 1425 1153 901 889 414 275 137 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3534 4435 1537 Size: 275 1722 901 4090 Fragments arranged by size: 4090 1722 901 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 2427 2472 2637 2817 3088 4918 1353 Size: 683 391 45 165 180 271 1830 3423 Fragments arranged by size: 3423 1830 683 391 271 180 165 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5575 5949 6095 387 Size: 1064 138 3986 374 146 1280 Fragments arranged by size: 3986 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3680 3680 Size: 6988 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2580 2639 3281 3488 4067 5051 Size: 393 421 527 59 642 207 579 984 Cuts at: 5051 5648 6435 6703 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 984 787 642 597 579 527 421 393 268 207 59 EcoNI CCTnn'n_nnAGG Cuts at: 2068 2873 2068 Size: 805 6183 EcoO109I rG'GnC_Cy Cuts at: 2083 4051 2083 Size: 1968 5020 EcoRI G'AATT_C Cuts at: 4297 4675 4297 Size: 378 6610 FspI TGC'GCA Cuts at: 5494 5494 Size: 6988 HaeII r_GCGC'y Cuts at: 1678 6135 1678 Size: 4457 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3770 3802 3902 3934 3965 Size: 32 135 32 2198 32 100 32 31 Cuts at: 3965 3997 4136 4168 5629 5661 5703 5735 1373 Size: 32 139 32 1461 32 42 32 2626 Fragments arranged by size: 2626 2198 1461 139 135 100 42 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2680 4332 4578 669 Size: 988 281 742 1652 246 3079 Fragments arranged by size: 3079 1652 988 742 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6988 HpaI GTT'AAC Cuts at: 2680 4578 2680 Size: 1898 5090 KpnI G_GTAC'C Cuts at: 3830 4313 3830 Size: 483 6505 MfeI C'AATT_G Cuts at: 3153 4587 3153 Size: 1434 5554 MluI A'CGCG_T Cuts at: 6864 6864 Size: 6988 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 2606 3843 3917 6102 6286 1305 Size: 774 527 1237 74 2185 184 2007 Fragments arranged by size: 2185 2007 1237 774 527 184 74 MscI TGG'CCA Cuts at: 10 64 1489 1832 2985 10 Size: 54 1425 343 1153 4013 Fragments arranged by size: 4013 1425 1153 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4946 5305 5464 518 Size: 648 3780 359 159 2042 Fragments arranged by size: 3780 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 3923 4404 5081 6229 6474 1606 Size: 474 1843 481 677 1148 245 2120 Fragments arranged by size: 2120 1843 1148 677 481 474 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6436 NdeI CA'TA_TG Cuts at: 387 387 Size: 6988 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6988 NotI GC'GGCC_GC Cuts at: 4435 4435 Size: 6988 NsiI A_TGCA'T Cuts at: 4042 4042 Size: 6988 NspI r_CATG'y Cuts at: 1132 1229 3886 4346 5891 1132 Size: 97 2657 460 1545 2229 Fragments arranged by size: 2657 2229 1545 460 97 PasI CC'CwG_GG Cuts at: 1348 3662 1348 Size: 2314 4674 PciI A'CATG_T Cuts at: 3882 5887 3882 Size: 2005 4983 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6988 PmeI GTTT'AAAC Cuts at: 4292 4292 Size: 6988 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3783 3815 5057 5089 6570 6602 3783 Size: 32 1242 32 1481 32 4169 Fragments arranged by size: 4169 1481 1242 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 4051 2083 Size: 1968 5020 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6988 PsiI TTA'TAA Cuts at: 4558 4558 Size: 6988 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3207 3239 3207 Size: 32 6956 PstI C_TGCA'G Cuts at: 830 3419 3553 4340 830 Size: 2589 134 787 3478 Fragments arranged by size: 3478 2589 787 134 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6988 PvuII CAG'CTG Cuts at: 3923 3923 Size: 6988 SacI G_AGCT'C Cuts at: 3682 3682 Size: 6988 SacII CC_GC'GG Cuts at: 2081 2081 Size: 6988 SalI G'TCGA_C Cuts at: 1936 4330 1936 Size: 2394 4594 SanDI GG'GwC_CC Cuts at: 2083 2083 Size: 6988 SapI GCTCTTCn'nnn_ Cuts at: 2036 2817 3088 2036 Size: 781 271 5936 Fragments arranged by size: 5936 781 271 SbfI CC_TGCA'GG Cuts at: 4340 4340 Size: 6988 ScaI AGT'ACT Cuts at: 3555 5236 6948 3555 Size: 1681 1712 3595 Fragments arranged by size: 3595 1712 1681 SfcI C'TryA_G Cuts at: 826 1045 2116 2206 3415 3549 4257 4336 5471 Size: 219 1071 90 1209 134 708 79 1135 Cuts at: 5471 6152 6343 826 Size: 681 191 1471 Fragments arranged by size: 1471 1209 1135 1071 708 681 219 191 134 90 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6988 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6988 SmaI CCC'GGG Cuts at: 1872 4315 1872 Size: 2443 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5092 5993 6255 820 Size: 197 926 3149 901 262 1553 Fragments arranged by size: 3149 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4779 493 Size: 4286 2702 SpeI A'CTAG_T Cuts at: 152 2213 152 Size: 2061 4927 SphI G_CATG'C Cuts at: 1132 4346 1132 Size: 3214 3774 SspI AAT'ATT Cuts at: 5 52 2259 3373 3586 4912 5 Size: 47 2207 1114 213 1326 2081 Fragments arranged by size: 2207 2081 1326 1114 213 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6988 StyI C'CwwG_G Cuts at: 513 1065 4427 513 Size: 552 3362 3074 Fragments arranged by size: 3362 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2095 5015 5032 5185 5370 5815 325 Size: 1255 515 2920 17 153 185 445 1498 Fragments arranged by size: 2920 1498 1255 515 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3409 3553 5234 Size: 274 80 33 51 1443 1432 144 1681 Cuts at: 5234 6946 96 Size: 1712 138 Fragments arranged by size: 1712 1681 1443 1432 274 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2768 2800 6948 6980 2768 Size: 32 4148 32 2776 Fragments arranged by size: 4148 2776 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6988 XbaI T'CTAG_A Cuts at: 3038 4324 3038 Size: 1286 5702 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6988 XmaI C'CCGG_G Cuts at: 1870 4313 1870 Size: 2443 4545 XmnI GAAnn'nnTTC Cuts at: 2646 5117 2646 Size: 2471 4517 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4796 276 Size: 53 83 186 936 3262 2468 Fragments arranged by size: 3262 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SexAI SfiI SfoI SrfI SwaI XcmI