(Linear) (Six-base) MAPSORT of: KIBB5770.seq Check: 153 from: 1 to: 2283 KIBB5770.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2009 2283 Size: 2009 274 AccI GT'mk_AC Cuts at: 0 291 2283 Size: 291 1992 Acc65I G'GTAC_C Cuts at: 0 1821 2283 Size: 1821 462 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 48 1276 2062 2283 Size: 48 1228 786 221 Fragments arranged by size: 1228 786 221 48 AflIII A'CryG_T Cuts at: 0 677 1877 2283 Size: 677 1200 406 Fragments arranged by size: 1200 677 406 AhdI GACnn_n'nnGTC Cuts at: 0 1911 2145 2283 Size: 1911 234 138 Fragments arranged by size: 1911 234 138 AlwNI CAG_nnn'CTG Cuts at: 0 609 1117 2283 Size: 609 508 1166 Fragments arranged by size: 1166 609 508 ApoI r'AATT_y Cuts at: 0 627 874 911 925 1304 1597 1620 1663 Size: 627 247 37 14 379 293 23 43 Cuts at: 1663 1846 2175 2283 Size: 183 329 108 Fragments arranged by size: 627 379 329 293 247 183 108 43 37 23 14 BanI G'GyrC_C Cuts at: 0 1821 2283 Size: 1821 462 BanII G_rGCy'C Cuts at: 0 1677 2283 Size: 1677 606 BbsI GAAGACnn'nnnn_ Cuts at: 0 1512 2283 Size: 1512 771 BclI T'GATC_A Cuts at: 0 144 2283 Size: 144 2139 BfrBI ATG'CAT Cuts at: 0 2035 2283 Size: 2035 248 BglII A'GATC_T Cuts at: 0 719 2283 Size: 719 1564 BlpI GC'TnA_GC Cuts at: 0 1919 2283 Size: 1919 364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 575 634 1483 2283 Size: 575 59 849 800 Fragments arranged by size: 849 800 575 59 BsaI GGTCTCn'nnnn_ Cuts at: 0 181 586 2283 Size: 181 405 1697 Fragments arranged by size: 1697 405 181 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1118 1148 2283 Size: 1118 30 1135 Fragments arranged by size: 1135 1118 30 BseYI C'CCAG_C Cuts at: 0 418 2283 Size: 418 1865 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 12 1436 2283 Size: 12 1424 847 Fragments arranged by size: 1424 847 12 BsiEI CG_ry'CG Cuts at: 0 1532 2283 Size: 1532 751 BsiHKAI G_wGCw'C Cuts at: 0 43 824 1677 1754 2283 Size: 43 781 853 77 529 Fragments arranged by size: 853 781 529 77 43 BsmI GAATG_Cn' Cuts at: 0 1554 2283 Size: 1554 729 Bsp1286I G_dGCh'C Cuts at: 0 43 824 1677 1754 2283 Size: 43 781 853 77 529 Fragments arranged by size: 853 781 529 77 43 BspHI T'CATG_A Cuts at: 0 1593 2283 Size: 1593 690 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2009 2283 Size: 2009 274 BsrBI CCG'CTC Cuts at: 0 2085 2283 Size: 2085 198 BsrDI GCAATG_nn' Cuts at: 0 411 2035 2283 Size: 411 1624 248 Fragments arranged by size: 1624 411 248 BsrFI r'CCGG_y Cuts at: 0 1531 2283 Size: 1531 752 BstXI CCAn_nnnn'nTGG Cuts at: 0 1623 2283 Size: 1623 660 BstYI r'GATC_y Cuts at: 0 719 2283 Size: 719 1564 BtgI C'CryG_G Cuts at: 0 73 2283 Size: 73 2210 BtsI GCAGTG_nn' Cuts at: 0 607 1419 1424 2283 Size: 607 812 5 859 Fragments arranged by size: 859 812 607 5 ClaI AT'CG_AT Cuts at: 0 165 1176 2283 Size: 165 1011 1107 Fragments arranged by size: 1107 1011 165 DrdI GACnn_nn'nnGTC Cuts at: 0 2105 2283 Size: 2105 178 EaeI y'GGCC_r Cuts at: 0 978 1392 1529 2283 Size: 978 414 137 754 Fragments arranged by size: 978 754 414 137 EagI C'GGCC_G Cuts at: 0 1529 2283 Size: 1529 754 EarI CTCTTCn'nnn_ Cuts at: 0 31 422 467 632 812 1083 2283 Size: 31 391 45 165 180 271 1200 Fragments arranged by size: 1200 391 271 180 165 45 31 EcoICRI GAG'CTC Cuts at: 0 1675 2283 Size: 1675 608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 48 575 634 1276 1483 2062 2283 Size: 48 527 59 642 207 579 221 Fragments arranged by size: 642 579 527 221 207 59 48 EcoNI CCTnn'n_nnAGG Cuts at: 0 63 868 2283 Size: 63 805 1415 Fragments arranged by size: 1415 805 63 EcoO109I rG'GnC_Cy Cuts at: 0 78 2046 2283 Size: 78 1968 237 Fragments arranged by size: 1968 237 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1765 1797 1897 1929 1960 1992 2131 2163 Size: 1765 32 100 32 31 32 139 32 Cuts at: 2163 2283 Size: 120 Fragments arranged by size: 1765 139 120 100 32 32 32 32 31 HincII GTy'rAC Cuts at: 0 675 2283 Size: 675 1608 HpaI GTT'AAC Cuts at: 0 675 2283 Size: 675 1608 KpnI G_GTAC'C Cuts at: 0 1825 2283 Size: 1825 458 MfeI C'AATT_G Cuts at: 0 1148 2283 Size: 1148 1135 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 74 601 1838 1912 2283 Size: 74 527 1237 74 371 Fragments arranged by size: 1237 527 371 74 74 MscI TGG'CCA Cuts at: 0 980 2283 Size: 980 1303 MspA1I CmG'CkG Cuts at: 0 75 1918 2283 Size: 75 1843 365 Fragments arranged by size: 1843 365 75 NsiI A_TGCA'T Cuts at: 0 2037 2283 Size: 2037 246 NspI r_CATG'y Cuts at: 0 1881 2283 Size: 1881 402 PasI CC'CwG_GG Cuts at: 0 1657 2283 Size: 1657 626 PciI A'CATG_T Cuts at: 0 1877 2283 Size: 1877 406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1778 1810 2283 Size: 1778 32 473 Fragments arranged by size: 1778 473 32 PpuMI rG'GwC_Cy Cuts at: 0 78 2046 2283 Size: 78 1968 237 Fragments arranged by size: 1968 237 78 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1202 1234 2283 Size: 1202 32 1049 Fragments arranged by size: 1202 1049 32 PstI C_TGCA'G Cuts at: 0 1414 1548 2283 Size: 1414 134 735 Fragments arranged by size: 1414 735 134 PvuII CAG'CTG Cuts at: 0 1918 2283 Size: 1918 365 SacI G_AGCT'C Cuts at: 0 1677 2283 Size: 1677 606 SacII CC_GC'GG Cuts at: 0 76 2283 Size: 76 2207 SanDI GG'GwC_CC Cuts at: 0 78 2283 Size: 78 2205 SapI GCTCTTCn'nnn_ Cuts at: 0 31 812 1083 2283 Size: 31 781 271 1200 Fragments arranged by size: 1200 781 271 31 ScaI AGT'ACT Cuts at: 0 1550 2283 Size: 1550 733 SfcI C'TryA_G Cuts at: 0 111 201 1410 1544 2252 2283 Size: 111 90 1209 134 708 31 Fragments arranged by size: 1209 708 134 111 90 31 SpeI A'CTAG_T Cuts at: 0 208 2283 Size: 208 2075 SspI AAT'ATT Cuts at: 0 254 1368 1581 2283 Size: 254 1114 213 702 Fragments arranged by size: 1114 702 254 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 90 2283 Size: 90 2193 TatI w'GTAC_w Cuts at: 0 1404 1548 2283 Size: 1404 144 735 Fragments arranged by size: 1404 735 144 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 763 795 2283 Size: 763 32 1488 Fragments arranged by size: 1488 763 32 XbaI T'CTAG_A Cuts at: 0 1033 2283 Size: 1033 1250 XmnI GAAnn'nnTTC Cuts at: 0 641 2283 Size: 641 1642 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AhdI AlwNI ApoI BanI BanII BbsI BclI BfrBI BglII BlpI BpmI BsaI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstXI BstYI BtgI BtsI ClaI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII HpaI KpnI MfeI MmeI MscI MspA1I NsiI NspI PasI PciI PpiI PpuMI PsrI PstI PvuII SacI SacII SanDI SapI ScaI SfcI SpeI SspI TaqII TatI TstI XbaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII KasI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PvuI RsrII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI StuI StyI SwaI Tth111I XcmI XhoI XmaI ZraI