################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 569 GTCTAC GT^[AC][GT]AC Aor51HI 1046 AGCGCT AGC^GCT ApaI 445 GGGCCC GGGCC^C BamHI 544 GGATCC G^GATCC BbvCI 480 CCTCAGC CCTCAGC(-5/-2) BglI 438 GCCACCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsmAI 591 GAGAC GTCTC(1/5) BspHI 227 TCATGA T^CATGA BsrBI 979 GAGCGG CCGCTC(-3/-3) BsrFI 55 ACCGGC [GA]^CCGG[CT] BsrI 1001 ACTGG ACTGG(1/-1) BssHII 603 GCGCGC G^CGCGC BstXI 779 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 544 GGATCC [GA]^GATC[CT] Cfr10I 55 ACCGGC [GA]^CCGG[CT] DdeI 481 CTCAG C^T[ACGT]AG DpnII 545 GATC ^GATC EarI 34 CTCTTC CTCTTC(1/4) Eco47III 1046 AGCGCT AGC^GCT EcoNI 1019 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 790 GATATC GAT^ATC Ksp632I 34 CTCTTC CTCTTC(1/4) MaeII 1013 ACGT A^CGT MaeIII 1015 GTCAC ^GT[ACGT]AC MboI 545 GATC ^GATC MflI 544 GGATCC [GA]^GATC[CT] NdeII 545 GATC ^GATC NotI 9 GCGGCCGC GC^GGCCGC NspI 257 ACATGC [GA]CATG^[CT] RcaI 227 TCATGA T^CATGA SapI 33 GCTCTTC GCTCTTC(1/4) Sau3AI 545 GATC ^GATC SexAI 770 ACCTGGT A^CC[AT]GGT SmlI 709 CTCAAG C^T[CT][GA]AG StuI 233 AGGCCT AGG^CCT TaqI 977 TCGA T^CGA Tsp45I 1015 GTCAC ^GT[GC]AC TthHB8I 977 TCGA T^CGA XcmI 633 CCATGGGGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 544 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 996 GTAC GT^AC 1037 GTAC Alw44I 208 GTGCAC G^TGCAC 742 GTGCAC AlwI 545 GATCC GGATC(4/5) 544 GGATC AlwNI 595 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 682 CAGGAGCTG ApaLI 208 GTGCAC G^TGCAC 742 GTGCAC AvaII 320 GGACC G^G[AT]CC 783 GGACC BseRI 273 CTCCTC GAGGAG(10/8) 493 CTCCTC BsiEI 10 CGGCCG CG[GA][CT]^CG 353 CGGCCG BstAPI 681 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 768 GCACCTGGTGC DraII 319 GGGACCC [GA]G^G[ACGT]CC[CT] 782 AGGACCT DraIII 199 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 769 CACCTGGTG DrdI 321 GACCCGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 967 GACCGCGTGGTC EagI 10 CGGCCG C^GGCCG 353 CGGCCG EclXI 10 CGGCCG C^GGCCG 353 CGGCCG Eco52I 10 CGGCCG C^GGCCG 353 CGGCCG EcoO109I 319 GGGACCC [GA]G^G[ACGT]CC[CT] 782 AGGACCT HgaI 123 GACGC GACGC(5/10) 567 GCGTC HinfI 328 GAGTC G^A[ACGT]TC 808 GACTC MboII 852 GAAGA GAAGA(8/7) 35 TCTTC MslI 201 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 574 CACAACAGTG NcoI 633 CCATGG C^CATGG 752 CCATGG PleI 808 GACTC GAGTC(4/5) 328 GAGTC PpuMI 319 GGGACCC [GA]G^G[AT]CC[CT] 782 AGGACCT RsaI 996 GTAC GT^AC 1037 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AluI AspHI AvaI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI PvuII SacI Sau96I ScrFI SfaNI SfcI SmaI StyI TseI TspRI XmaCI XmaI