(Circular) (Six-base) MAPSORT of: pFN21AE1837.seq Check: 8957 from: 1 to: 5767 pFN21AE1837 5767 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2678 3021 3032 2678 Size: 343 11 5413 Fragments arranged by size: 5413 343 11 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2575 3110 1937 Size: 638 535 4594 Fragments arranged by size: 4594 638 535 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3830 5214 5482 1632 Size: 2198 1384 268 1917 Fragments arranged by size: 2198 1917 1384 268 AfeI AGC'GCT Cuts at: 3053 3053 Size: 5767 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 4666 5643 1203 Size: 3463 977 1327 Fragments arranged by size: 3463 1327 977 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 2210 2583 1166 Size: 1044 373 4350 Fragments arranged by size: 4350 1044 373 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5767 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2264 2296 2264 Size: 32 5735 AlwNI CAG_nnn'CTG Cuts at: 2605 2692 3510 5082 2605 Size: 87 818 1572 3290 Fragments arranged by size: 3290 1572 818 87 ApaI G_GGCC'C Cuts at: 2454 2454 Size: 5767 ApaLI G'TGCA_C Cuts at: 2213 2747 3824 4980 2213 Size: 534 1077 1156 3000 Fragments arranged by size: 3000 1156 1077 534 ApoI r'AATT_y Cuts at: 1493 3076 3302 3454 5563 1493 Size: 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 2328 2385 2447 2453 3092 1870 Size: 73 385 57 62 6 639 4545 Fragments arranged by size: 4545 639 385 73 62 57 6 BamHI G'GATC_C Cuts at: 2549 3097 3464 2549 Size: 548 367 4852 Fragments arranged by size: 4852 548 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2762 2779 2988 3088 4543 Size: 325 217 627 975 17 209 100 1455 Cuts at: 4543 618 Size: 1842 Fragments arranged by size: 1842 1455 975 627 325 217 209 100 17 BanII G_rGCy'C Cuts at: 1391 2199 2292 2454 2499 2840 2876 3086 1391 Size: 808 93 162 45 341 36 210 4072 Fragments arranged by size: 4072 808 341 210 162 93 45 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4796 BbvCI CC'TCA_GC Cuts at: 2486 2486 Size: 5767 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3958 3992 1903 Size: 34 2021 34 3678 Fragments arranged by size: 3678 2021 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3659 4869 3659 Size: 1210 4557 BclI T'GATC_A Cuts at: 1548 1548 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2449 4378 Size: 107 122 71 631 605 337 440 1929 Cuts at: 4378 136 Size: 1525 Fragments arranged by size: 1929 1525 631 605 440 337 122 107 71 BglII A'GATC_T Cuts at: 5762 5762 Size: 5767 BlpI GC'TnA_GC Cuts at: 3184 3184 Size: 5767 Bme1580I G_kGCm'C Cuts at: 1790 2217 2454 2751 2765 2784 3828 4984 1790 Size: 427 237 297 14 19 1044 1156 2573 Fragments arranged by size: 2573 1156 1044 427 297 237 19 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3498 4456 442 Size: 416 53 83 399 24 2081 958 1753 Fragments arranged by size: 2081 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2619 2619 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2168 2213 2336 4427 1239 Size: 800 129 45 123 2091 2579 Fragments arranged by size: 2579 2091 800 129 123 45 Bpu10I CC'TnA_GC Cuts at: 2486 2486 Size: 5767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2699 3892 4757 5055 2699 Size: 1193 865 298 3411 Fragments arranged by size: 3411 1193 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4430 882 Size: 617 2931 2219 Fragments arranged by size: 2931 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 5761 5761 Size: 5767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3574 3956 4591 275 Size: 53 83 186 936 2041 382 635 1451 Fragments arranged by size: 2041 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4200 4598 4872 5019 5430 4200 Size: 398 274 147 411 4537 Fragments arranged by size: 4537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 1178 Size: 30 165 30 861 30 4651 Fragments arranged by size: 4651 861 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2269 2489 1186 Size: 1083 220 4464 Fragments arranged by size: 4464 1083 220 BseYI C'CCAG_C Cuts at: 2463 4970 5466 2463 Size: 2507 496 2764 Fragments arranged by size: 2764 2507 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2148 2192 2377 1163 Size: 573 412 44 185 4553 Fragments arranged by size: 4553 573 412 185 44 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2018 2361 3217 3978 5006 1540 Size: 275 186 17 343 856 761 1028 2301 Fragments arranged by size: 2301 1028 856 761 343 275 186 17 BsiHKAI G_wGCw'C Cuts at: 2199 2217 2292 2751 2840 3828 3913 4984 2199 Size: 18 75 459 89 988 85 1071 2982 Fragments arranged by size: 2982 1071 988 459 89 85 75 18 BsmI GAATG_Cn' Cuts at: 3278 3371 3278 Size: 93 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2217 2292 2454 2499 2751 2765 Size: 399 409 18 75 162 45 252 14 Cuts at: 2765 2784 2840 2876 3086 3828 3913 4984 1391 Size: 19 56 36 210 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 409 399 252 210 162 85 75 56 45 36 19 18 14 BspEI T'CCGG_A Cuts at: 5430 5430 Size: 5767 BspHI T'CATG_A Cuts at: 2232 3656 5386 2232 Size: 1424 1730 2613 Fragments arranged by size: 2613 1730 1424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2011 2415 2678 3021 3032 3122 844 Size: 1167 404 263 343 11 90 3489 Fragments arranged by size: 3489 1167 404 343 263 90 11 BsrBI CCG'CTC Cuts at: 1394 1823 2986 3654 1394 Size: 429 1163 668 3507 Fragments arranged by size: 3507 1163 668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4262 4436 65 Size: 1183 3014 174 1396 Fragments arranged by size: 3014 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 4411 4598 1700 Size: 110 250 2351 187 2869 Fragments arranged by size: 2869 2351 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5767 BssHII G'CGCG_C Cuts at: 1925 2608 1925 Size: 683 5084 BssSI C'ACGA_G Cuts at: 3827 4839 3827 Size: 1012 4755 BstAPI GCAn_nnn'nTGC Cuts at: 794 2692 2779 794 Size: 1898 87 3782 Fragments arranged by size: 3782 1898 87 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstXI CCAn_nnnn'nTGG Cuts at: 2791 2791 Size: 5767 BstYI r'GATC_y Cuts at: 1972 2549 3097 3464 3849 3866 5318 5404 5416 Size: 577 548 367 385 17 1452 86 12 Cuts at: 5416 5762 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 577 548 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2638 2757 2878 3045 513 Size: 552 1573 119 121 167 3235 Fragments arranged by size: 3235 1573 552 167 121 119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2365 487 Size: 683 56 1139 3889 Fragments arranged by size: 3889 1139 683 56 BtsI GCAGTG_nn' Cuts at: 2134 2903 3283 4077 4097 2134 Size: 769 380 794 20 3804 Fragments arranged by size: 3804 794 769 380 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DraIII CAC_nnn'GTG Cuts at: 2209 2779 2209 Size: 570 5197 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2332 2978 4774 809 Size: 507 1016 646 1796 1802 Fragments arranged by size: 1802 1796 1016 646 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 2358 2733 Size: 54 1425 50 275 18 185 343 375 Cuts at: 2733 3214 4103 5732 8 Size: 481 889 1629 43 Fragments arranged by size: 1629 1425 889 481 375 343 275 185 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2015 2358 3214 1537 Size: 275 203 343 856 4090 Fragments arranged by size: 4090 856 343 275 203 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 3697 1353 Size: 692 1652 3423 Fragments arranged by size: 3423 1652 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4354 4728 4874 387 Size: 1064 138 2765 374 146 1280 Fragments arranged by size: 2765 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2197 2290 2838 2197 Size: 93 548 5126 Fragments arranged by size: 5126 548 93 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2168 2213 2336 3830 4427 5214 Size: 393 407 129 45 123 1494 597 787 Cuts at: 5214 5482 1239 Size: 268 1524 Fragments arranged by size: 1524 1494 787 597 407 393 268 129 123 45 EcoNI CCTnn'n_nnAGG Cuts at: 3028 3028 Size: 5767 EcoO109I rG'GnC_Cy Cuts at: 2325 2788 2325 Size: 463 5304 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 EcoRV GAT'ATC Cuts at: 2797 2797 Size: 5767 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 2036 2054 2093 3055 4914 1678 Size: 358 18 39 962 1859 2531 Fragments arranged by size: 2531 1859 962 358 39 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4408 4440 4482 4514 1373 Size: 32 135 32 2836 32 42 32 2626 Fragments arranged by size: 2836 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2937 4881 5065 1305 Size: 1632 1944 184 2007 Fragments arranged by size: 2007 1944 1632 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3945 Fragments arranged by size: 3945 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2583 3725 4084 4243 518 Size: 648 1044 373 1142 359 159 2042 Fragments arranged by size: 2042 1142 1044 648 373 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2176 2463 2928 3183 3860 5008 5253 Size: 540 30 287 465 255 677 1148 245 Cuts at: 5253 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 540 465 287 255 245 30 NcoI C'CATG_G Cuts at: 513 1065 2638 2757 513 Size: 552 1573 119 3523 Fragments arranged by size: 3523 1573 552 119 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 2015 3214 2015 Size: 1199 4568 NspI r_CATG'y Cuts at: 1132 1229 2266 3125 4670 1132 Size: 97 1037 859 1545 2229 Fragments arranged by size: 2229 1545 1037 859 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5767 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5767 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2264 2296 3836 3868 5349 5381 2264 Size: 32 1540 32 1481 32 2650 Fragments arranged by size: 2650 1540 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2325 2788 2325 Size: 463 5304 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PspOMI G'GGCC_C Cuts at: 2450 2450 Size: 5767 PstI C_TGCA'G Cuts at: 830 2688 2697 2835 3119 830 Size: 1858 9 138 284 3478 Fragments arranged by size: 3478 1858 284 138 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 PvuII CAG'CTG Cuts at: 2146 2176 2928 2146 Size: 30 752 4985 Fragments arranged by size: 4985 752 30 SacI G_AGCT'C Cuts at: 2199 2292 2840 2199 Size: 93 548 5126 Fragments arranged by size: 5126 548 93 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SanDI GG'GwC_CC Cuts at: 2325 2325 Size: 5767 SapI GCTCTTCn'nnn_ Cuts at: 2045 2045 Size: 5767 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SexAI A'CCwGG_T Cuts at: 2775 2775 Size: 5767 SfcI C'TryA_G Cuts at: 826 1045 2684 2693 2831 3115 4250 4931 5122 Size: 219 1639 9 138 284 1135 681 191 Cuts at: 5122 826 Size: 1471 Fragments arranged by size: 1639 1471 1135 681 284 219 191 138 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 2330 2387 2449 2455 3094 1872 Size: 458 57 62 6 639 4545 Fragments arranged by size: 4545 639 458 62 57 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2714 3871 4772 5034 820 Size: 197 926 771 1157 901 262 1553 Fragments arranged by size: 1553 1157 926 901 771 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SrfI GCCC'GGGC Cuts at: 2387 2387 Size: 5767 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 2240 1517 Size: 723 5044 StyI C'CwwG_G Cuts at: 513 1065 2638 2739 2757 2784 3206 513 Size: 552 1573 101 18 27 422 3074 Fragments arranged by size: 3074 1573 552 422 101 27 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3794 3811 3964 4149 4594 325 Size: 1255 2214 17 153 185 445 1498 Fragments arranged by size: 2214 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4013 5725 96 Size: 274 80 33 51 1443 2036 1712 138 Fragments arranged by size: 2036 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2612 2644 5727 5759 2612 Size: 32 3083 32 2620 Fragments arranged by size: 3083 2620 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2645 2645 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5767 XmaI C'CCGG_G Cuts at: 1870 2328 2385 2447 2453 3092 1870 Size: 458 57 62 6 639 4545 Fragments arranged by size: 4545 639 458 62 57 6 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SfiI SfoI SwaI