################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BamHI BbvCI BcgI BciVI BclI BfaI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiHKAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MaeI MaeIII MamI MfeI MflI MroI MslI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AfaI 24 GTAC GT^AC AflII 751 CTTAAG C^TTAAG AflIII 355 ACGCGT A^C[GA][CT]GT ApaI 784 GGGCCC GGGCC^C AvaI 781 CCCGGG C^[CT]CG[GA]G AvaII 655 GGACC G^G[AT]CC AviII 169 TGCGCA TGC^GCA BalI 599 TGGCCA TGG^CCA BbrPI 540 CACGTG CAC^GTG BbsI 837 GAAGAC GAAGAC(2/6) BfrI 751 CTTAAG C^TTAAG BpmI 313 CTGGAG CTGGAG(16/14) BpuAI 837 GAAGAC GAAGAC(2/6) BsaAI 540 CACGTG [CT]AC^GT[GA] BsaI 331 GAGACC GGTCTC(1/5) BsaWI 815 ACCGGA [AT]^CCGG[AT] BsiEI 740 CGGCCG CG[GA][CT]^CG BsiWI 23 CGTACG C^GTACG BsmAI 331 GAGAC GTCTC(1/5) BsmFI 654 GGGAC GGGAC(10/14) BsoBI 781 CCCGGG C^[CT]CG[GA]G BspMI 402 GCAGGT ACCTGC(4/8) BsrI 639 ACTGG ACTGG(1/-1) DpnII 378 GATC ^GATC EagI 740 CGGCCG C^GGCCG EarI 847 CTCTTC CTCTTC(1/4) EclXI 740 CGGCCG C^GGCCG Eco52I 740 CGGCCG C^GGCCG EcoNI 384 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 169 TGCGCA TGC^GCA HgaI 52 GCGTC GACGC(5/10) Ksp632I 847 CTCTTC CTCTTC(1/4) KspI 128 CCGCGG CCGC^GG MboI 378 GATC ^GATC MluI 355 ACGCGT A^CGCGT MluNI 599 TGGCCA TGG^CCA MscI 599 TGGCCA TGG^CCA MseI 752 TTAA T^TAA NaeI 765 GCCGGC GCC^GGC NcoI 44 CCATGG C^CATGG NdeII 378 GATC ^GATC NgoMIV 765 GCCGGC G^CCGGC NlaIII 45 CATG CATG^ PmaCI 540 CACGTG CAC^GTG PmlI 540 CACGTG CAC^GTG PpuMI 654 GGGACCT [GA]G^G[AT]CC[CT] PstI 78 CTGCAG CTGCA^G RsaI 24 GTAC GT^AC SacII 128 CCGCGG CCGC^GG Sau3AI 378 GATC ^GATC SfaNI 275 GCATC GCATC(5/9) SfcI 78 CTGCAG C^T[GA][CT]AG SmaI 781 CCCGGG CCC^GGG TaqI 380 TCGA T^CGA TfiI 526 GATTC G^A[AT]TC Tru9I 752 TTAA T^TAA Tsp509I 19 AATT ^AATT TthHB8I 380 TCGA T^CGA XmaCI 781 CCCGGG C^CCGGG XmaI 781 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsrBI 676 CCGCTC CCGCTC(-3/-3) 737 GAGCGG BsrFI 697 GCCGGT [GA]^CCGG[CT] 765 GCCGGC BssSI 253 CACGAG CACGAG(-5/-1) 30 CTCGTG BstF5I 242 CATCC GGATG(2/0) 276 CATCC Cfr10I 697 GCCGGT [GA]^CCGG[CT] 765 GCCGGC DraII 654 GGGACCT [GA]G^G[ACGT]CC[CT] 784 GGGCCCT DsaI 44 CCATGG C^C[GA][CT]GG 128 CCGCGG EcoO109I 654 GGGACCT [GA]G^G[ACGT]CC[CT] 784 GGGCCCT FokI 242 CATCC GGATG(9/13) 276 CATCC HphI 290 TCACC GGTGA(8/7) 813 TCACC MaeII 286 ACGT A^CGT 541 ACGT ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcyI AluI BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BseRI BsgI BsiYI BslI Bsp1286I BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI EaeI EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV PleI Sau96I ScrFI SfoI SmlI StyI TseI TspRI