(Linear) (Six-base) MAPSORT of: KIBB7576.seq Check: 5684 from: 1 to: 852 KIBB7576.orf With 182 enzymes: SgfI * January 29, 2014 11:43 .. AflII C'TTAA_G Cuts at: 0 751 852 Size: 751 101 AflIII A'CryG_T Cuts at: 0 355 852 Size: 355 497 ApaI G_GGCC'C Cuts at: 0 788 852 Size: 788 64 AvaI C'yCGr_G Cuts at: 0 781 852 Size: 781 71 BanI G'GyrC_C Cuts at: 0 73 178 484 852 Size: 73 105 306 368 Fragments arranged by size: 368 306 105 73 BanII G_rGCy'C Cuts at: 0 619 646 788 852 Size: 619 27 142 64 Fragments arranged by size: 619 142 64 27 BbeI G_GCGC'C Cuts at: 0 77 182 488 852 Size: 77 105 306 364 Fragments arranged by size: 364 306 105 77 BbsI GAAGACnn'nnnn_ Cuts at: 0 844 852 Size: 844 8 BglI GCCn_nnn'nGGC Cuts at: 0 49 89 703 852 Size: 49 40 614 149 Fragments arranged by size: 614 149 49 40 Bme1580I G_kGCm'C Cuts at: 0 221 788 852 Size: 221 567 64 Fragments arranged by size: 567 221 64 BmrI ACTGGGnnnn_n' Cuts at: 0 649 852 Size: 649 203 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 852 Size: 334 518 Bpu10I CC'TnA_GC Cuts at: 0 613 852 Size: 613 239 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 358 664 852 Size: 289 69 306 188 Fragments arranged by size: 306 289 188 69 BsaI GGTCTCn'nnnn_ Cuts at: 0 325 852 Size: 325 527 BsaAI yAC'GTr Cuts at: 0 542 852 Size: 542 310 BsaHI Gr'CG_yC Cuts at: 0 52 74 179 485 852 Size: 52 22 105 306 367 Fragments arranged by size: 367 306 105 52 22 BsaWI w'CCGG_w Cuts at: 0 815 852 Size: 815 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 526 556 852 Size: 526 30 296 Fragments arranged by size: 526 296 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 150 376 501 852 Size: 150 226 125 351 Fragments arranged by size: 351 226 150 125 BseYI C'CCAG_C Cuts at: 0 619 852 Size: 619 233 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 421 564 852 Size: 235 186 143 288 Fragments arranged by size: 288 235 186 143 BsiEI CG_ry'CG Cuts at: 0 743 852 Size: 743 109 BsiWI C'GTAC_G Cuts at: 0 23 852 Size: 23 829 Bsp1286I G_dGCh'C Cuts at: 0 221 619 646 788 852 Size: 221 398 27 142 64 Fragments arranged by size: 398 221 142 64 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 393 852 Size: 393 459 BsrBI CCG'CTC Cuts at: 0 678 739 852 Size: 678 61 113 Fragments arranged by size: 678 113 61 BsrFI r'CCGG_y Cuts at: 0 697 765 852 Size: 697 68 87 Fragments arranged by size: 697 87 68 BssHII G'CGCG_C Cuts at: 0 296 447 502 852 Size: 296 151 55 350 Fragments arranged by size: 350 296 151 55 BssSI C'ACGA_G Cuts at: 0 30 253 852 Size: 30 223 599 Fragments arranged by size: 599 223 30 BtgI C'CryG_G Cuts at: 0 44 128 852 Size: 44 84 724 Fragments arranged by size: 724 84 44 BtsI GCAGTG_nn' Cuts at: 0 221 852 Size: 221 631 EaeI y'GGCC_r Cuts at: 0 41 452 599 740 852 Size: 41 411 147 141 112 Fragments arranged by size: 411 147 141 112 41 EagI C'GGCC_G Cuts at: 0 740 852 Size: 740 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 45 784 852 Size: 45 739 68 Fragments arranged by size: 739 68 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 852 Size: 334 518 EcoNI CCTnn'n_nnAGG Cuts at: 0 388 852 Size: 388 464 EcoO109I rG'GnC_Cy Cuts at: 0 655 785 852 Size: 655 130 67 Fragments arranged by size: 655 130 67 FspI TGC'GCA Cuts at: 0 171 852 Size: 171 681 HaeII r_GCGC'y Cuts at: 0 77 182 373 461 488 852 Size: 77 105 191 88 27 364 Fragments arranged by size: 364 191 105 88 77 27 KasI G'GCGC_C Cuts at: 0 73 178 484 852 Size: 73 105 306 368 Fragments arranged by size: 368 306 105 73 MluI A'CGCG_T Cuts at: 0 355 852 Size: 355 497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 188 852 Size: 188 664 MscI TGG'CCA Cuts at: 0 601 852 Size: 601 251 MspA1I CmG'CkG Cuts at: 0 130 270 627 852 Size: 130 140 357 225 Fragments arranged by size: 357 225 140 130 NaeI GCC'GGC Cuts at: 0 767 852 Size: 767 85 NarI GG'CG_CC Cuts at: 0 74 179 485 852 Size: 74 105 306 367 Fragments arranged by size: 367 306 105 74 NcoI C'CATG_G Cuts at: 0 44 852 Size: 44 808 NgoMIV G'CCGG_C Cuts at: 0 765 852 Size: 765 87 PmlI CAC'GTG Cuts at: 0 542 852 Size: 542 310 PpuMI rG'GwC_Cy Cuts at: 0 655 852 Size: 655 197 PspOMI G'GGCC_C Cuts at: 0 784 852 Size: 784 68 PstI C_TGCA'G Cuts at: 0 82 852 Size: 82 770 SacII CC_GC'GG Cuts at: 0 131 852 Size: 131 721 SfcI C'TryA_G Cuts at: 0 78 852 Size: 78 774 SfoI GGC'GCC Cuts at: 0 75 180 486 852 Size: 75 105 306 366 Fragments arranged by size: 366 306 105 75 SmaI CCC'GGG Cuts at: 0 783 852 Size: 783 69 SmlI C'TyrA_G Cuts at: 0 304 373 679 751 852 Size: 304 69 306 72 101 Fragments arranged by size: 306 304 101 72 69 StyI C'CwwG_G Cuts at: 0 44 461 530 852 Size: 44 417 69 322 Fragments arranged by size: 417 322 69 44 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 526 558 852 Size: 526 32 294 Fragments arranged by size: 526 294 32 XmaI C'CCGG_G Cuts at: 0 781 852 Size: 781 71 Enzymes that do cut: AflII AflIII ApaI AvaI BanI BanII BbeI BbsI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BtgI BtsI EaeI EagI EciI Eco57MI EcoNI EcoO109I FspI HaeII KasI MluI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV PmlI PpuMI PspOMI PstI SacII SfcI SfoI SmaI SmlI StyI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaBI BsiHKAI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MslI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI ZraI