Restriction map for
pFN21AE5171
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaJI BsaWI
BseAI BsgI BsiEI BsiWI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI KspI MaeII
MaeIII MamI MfeI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Alw44I 422 GTGCAC G^TGCAC
AlwNI 151 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 422 GTGCAC G^TGCAC
AspHI 422 GTGCAC G[AT]GC[AT]^C
BalI 356 TGGCCA TGG^CCA
BamHI 476 GGATCC G^GATCC
BfaI 74 CTAG C^TAG
BlpI 282 GCTGAGC GC^T[ACGT]AGC
BpmI 396 CTCCAG CTGGAG(16/14)
Bpu1102I 282 GCTGAGC GC^T[ACGT]AGC
BsaI 393 GGTCTC GGTCTC(1/5)
BsiHKAI 422 GTGCAC G[AT]GC[AT]^C
BsiYI 348 CCAAGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 348 CCAAGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 326 CGTCTC CGTCTC(1/5)
Bsp1407I 465 TGTACA T^GTACA
BspMI 390 GCAGGT ACCTGC(4/8)
BsrGI 465 TGTACA T^GTACA
BsrI 359 CCAGT ACTGG(1/-1)
BstAPI 150 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 282 GCTGAGC GC^T[ACGT]AGC
DraII 152 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 346 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 356 TGGCCA [CT]^GGCC[GA]
EarI 162 CTCTTC CTCTTC(1/4)
EcoO109I 152 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 269 CCCGC CCCGC(4/6)
HgaI 445 GACGC GACGC(5/10)
Ksp632I 162 CTCTTC CTCTTC(1/4)
MaeI 74 CTAG C^TAG
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MslI 195 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 319 CCGCTG C[AC]G^C[GT]G
PflMI 348 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 209 CTGCAG CTGCA^G
SspBI 465 TGTACA T^GTACA
Van91I 348 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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BanII 109 GAGCCC G[GA]GC[CT]^C
364 GAGCCC
BseRI 49 GAGGAG GAGGAG(10/8)
454 GAGGAG
BsmAI 327 GTCTC GTCTC(1/5)
394 GTCTC
BsrBI 134 CCGCTC CCGCTC(-3/-3)
52 GAGCGG
BstF5I 168 CATCC GGATG(2/0)
369 CATCC
BstYI 434 AGATCC [GA]^GATC[CT]
476 GGATCC
DdeI 283 CTGAG C^T[ACGT]AG
431 CTCAG
DpnII 435 GATC ^GATC
477 GATC
FokI 168 CATCC GGATG(9/13)
369 CATCC
HaeIII 153 GGCC GG^CC
357 GGCC
HinfI 31 GACTC G^A[ACGT]TC
214 GACTC
MboI 435 GATC ^GATC
477 GATC
MboII 306 GAAGA GAAGA(8/7)
163 TCTTC
MflI 434 AGATCC [GA]^GATC[CT]
476 GGATCC
NdeII 435 GATC ^GATC
477 GATC
PleI 31 GACTC GAGTC(4/5)
214 GACTC
Sau3AI 435 GATC ^GATC
477 GATC
XhoII 434 AGATCC [GA]^GATC[CT]
476 GGATCC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BmyI Bsp1286I BssKI BstNI
Cac8I Cfr13I EcoRII Fnu4HI ItaI
MnlI MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI SfcI TseI
TspRI