(Circular) (Six-base) MAPSORT of: pFN21AE5171.seq Check: 3268 from: 1 to: 5188 pFN21AE5171 5188 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2998 278 Size: 53 83 186 936 1462 2468 Fragments arranged by size: 2468 1462 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2531 1937 Size: 594 4594 Acc65I G'GTAC_C Cuts at: 2509 2509 Size: 5188 AclI AA'CG_TT Cuts at: 1563 3315 3688 1563 Size: 1752 373 3063 Fragments arranged by size: 3063 1752 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3251 4635 4903 1632 Size: 1619 1384 268 1917 Fragments arranged by size: 1917 1619 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4991 AflIII A'CryG_T Cuts at: 1203 4087 5064 1203 Size: 2884 977 1327 Fragments arranged by size: 2884 1327 977 AgeI A'CCGG_T Cuts at: 4019 4019 Size: 5188 AhdI GACnn_n'nnGTC Cuts at: 3917 3917 Size: 5188 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5188 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5188 AlwNI CAG_nnn'CTG Cuts at: 2161 2931 4503 2161 Size: 770 1572 2846 Fragments arranged by size: 2846 1572 770 ApaLI G'TGCA_C Cuts at: 2427 3245 4401 2427 Size: 818 1156 3214 Fragments arranged by size: 3214 1156 818 ApoI r'AATT_y Cuts at: 1493 2497 2723 2875 4984 1493 Size: 1004 226 152 2109 1697 Fragments arranged by size: 2109 1697 1004 226 152 AseI AT'TA_AT Cuts at: 160 1032 3742 160 Size: 872 2710 1606 Fragments arranged by size: 2710 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5188 AvaI C'yCGr_G Cuts at: 1870 1943 2513 1870 Size: 73 570 4545 Fragments arranged by size: 4545 570 73 BamHI G'GATC_C Cuts at: 2481 2518 2885 2481 Size: 37 367 4784 Fragments arranged by size: 4784 367 37 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2509 3964 618 Size: 325 217 627 722 1455 1842 Fragments arranged by size: 1842 1455 722 627 325 217 BanII G_rGCy'C Cuts at: 1391 2118 2373 2507 1391 Size: 727 255 134 4072 Fragments arranged by size: 4072 727 255 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3379 3413 1903 Size: 34 1442 34 3678 Fragments arranged by size: 3678 1442 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3080 4290 3080 Size: 1210 3978 BclI T'GATC_A Cuts at: 1548 1548 Size: 5188 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3799 136 Size: 107 122 71 631 605 2127 1525 Fragments arranged by size: 2127 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5183 5183 Size: 5188 BlpI GC'TnA_GC Cuts at: 2288 2605 2288 Size: 317 4871 Bme1580I G_kGCm'C Cuts at: 1790 2431 3249 4405 1790 Size: 641 818 1156 2573 Fragments arranged by size: 2573 1156 818 641 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5188 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2480 2919 3877 Size: 416 53 83 399 24 1063 439 958 Cuts at: 3877 442 Size: 1753 Fragments arranged by size: 1753 1063 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5188 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2386 3848 1239 Size: 1147 1462 2579 Fragments arranged by size: 2579 1462 1147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3313 4178 4476 3313 Size: 865 298 4025 Fragments arranged by size: 4025 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2404 3851 882 Size: 617 905 1447 2219 Fragments arranged by size: 2219 1447 905 617 BsaAI yAC'GTr Cuts at: 493 1204 2979 493 Size: 711 1775 2702 Fragments arranged by size: 2702 1775 711 BsaBI GATnn'nnATC Cuts at: 5182 5182 Size: 5188 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2995 3377 4012 275 Size: 53 83 186 936 1462 382 635 1451 Fragments arranged by size: 1462 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3621 4019 4293 4440 4851 3621 Size: 398 274 147 411 3958 Fragments arranged by size: 3958 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4963 Fragments arranged by size: 4963 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2474 1186 Size: 883 405 3900 Fragments arranged by size: 3900 883 405 BseYI C'CCAG_C Cuts at: 4391 4887 4391 Size: 496 4692 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4615 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2638 3399 4427 1540 Size: 275 186 637 761 1028 2301 Fragments arranged by size: 2301 1028 761 637 275 186 BsiHKAI G_wGCw'C Cuts at: 2431 3249 3334 4405 2431 Size: 818 85 1071 3214 Fragments arranged by size: 3214 1071 818 85 BsmI GAATG_Cn' Cuts at: 2699 2792 2699 Size: 93 5095 BsmBI CGTCTCn'nnnn_ Cuts at: 2337 2337 Size: 5188 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2373 2431 2507 3249 3334 4405 Size: 399 328 255 58 76 742 85 1071 Cuts at: 4405 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 328 255 85 76 58 BspEI T'CCGG_A Cuts at: 4851 4851 Size: 5188 BspHI T'CATG_A Cuts at: 3077 4807 3077 Size: 1730 3458 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2386 2543 844 Size: 1542 157 3489 Fragments arranged by size: 3489 1542 157 BsrBI CCG'CTC Cuts at: 1394 1823 2059 2141 3075 1394 Size: 429 236 82 934 3507 Fragments arranged by size: 3507 934 429 236 82 BsrDI GCAATG_nn' Cuts at: 65 1248 3683 3857 65 Size: 1183 2435 174 1396 Fragments arranged by size: 2435 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3832 4019 1700 Size: 110 2022 187 2869 Fragments arranged by size: 2869 2022 187 110 BsrGI T'GTAC_A Cuts at: 96 2470 96 Size: 2374 2814 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5188 BssSI C'ACGA_G Cuts at: 3248 4260 3248 Size: 1012 4176 BstAPI GCAn_nnn'nTGC Cuts at: 794 2161 794 Size: 1367 3821 BstBI TT'CG_AA Cuts at: 3992 3992 Size: 5188 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5188 BstYI r'GATC_y Cuts at: 1972 2439 2481 2518 2885 3270 3287 4739 4825 Size: 467 42 37 367 385 17 1452 86 Cuts at: 4825 4837 5183 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 467 385 367 346 86 42 37 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3697 Fragments arranged by size: 3697 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4449 Fragments arranged by size: 4449 683 56 BtsI GCAGTG_nn' Cuts at: 2704 3498 3518 2704 Size: 794 20 4374 Fragments arranged by size: 4374 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5188 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5153 DraI TTT'AAA Cuts at: 2492 2492 Size: 5188 DraIII CAC_nnn'GTG Cuts at: 2356 2356 Size: 5188 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4195 809 Size: 507 2879 1802 Fragments arranged by size: 2879 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2635 3524 Size: 54 1425 50 275 18 531 274 889 Cuts at: 3524 5153 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 531 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2635 1537 Size: 275 823 4090 Fragments arranged by size: 4090 823 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2173 3118 1353 Size: 820 945 3423 Fragments arranged by size: 3423 945 820 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3775 4149 4295 387 Size: 1064 138 2186 374 146 1280 Fragments arranged by size: 2186 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2386 3251 3848 4635 4903 1239 Size: 393 754 865 597 787 268 1524 Fragments arranged by size: 1524 865 787 754 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2158 2158 Size: 5188 EcoRI G'AATT_C Cuts at: 2497 2875 2497 Size: 378 4810 FspI TGC'GCA Cuts at: 3694 3694 Size: 5188 HaeII r_GCGC'y Cuts at: 1678 4335 1678 Size: 2657 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3829 3861 3903 3935 1373 Size: 32 135 32 2257 32 42 32 2626 Fragments arranged by size: 2626 2257 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2532 2778 669 Size: 988 281 594 246 3079 Fragments arranged by size: 3079 988 594 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5188 HpaI GTT'AAC Cuts at: 2778 2778 Size: 5188 KpnI G_GTAC'C Cuts at: 2513 2513 Size: 5188 MfeI C'AATT_G Cuts at: 2787 2787 Size: 5188 MluI A'CGCG_T Cuts at: 5064 5064 Size: 5188 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4302 4486 1305 Size: 2997 184 2007 Fragments arranged by size: 2997 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 2835 Fragments arranged by size: 2835 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 3146 3505 3664 518 Size: 648 1038 942 359 159 2042 Fragments arranged by size: 2042 1038 942 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2326 2604 3281 4429 4674 1606 Size: 720 278 677 1148 245 2120 Fragments arranged by size: 2120 1148 720 677 278 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3697 Fragments arranged by size: 3697 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5188 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5188 NotI GC'GGCC_GC Cuts at: 2635 2635 Size: 5188 NspI r_CATG'y Cuts at: 1132 1229 2546 4091 1132 Size: 97 1317 1545 2229 Fragments arranged by size: 2229 1545 1317 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5188 PciI A'CATG_T Cuts at: 4087 4087 Size: 5188 PflMI CCAn_nnn'nTGG Cuts at: 1108 2359 1108 Size: 1251 3937 PfoI T'CCnGG_A Cuts at: 2402 2402 Size: 5188 PmeI GTTT'AAAC Cuts at: 2492 2492 Size: 5188 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2021 2053 3257 3289 4770 4802 2021 Size: 32 1204 32 1481 32 2407 Fragments arranged by size: 2407 1481 1204 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5188 PsiI TTA'TAA Cuts at: 2758 2758 Size: 5188 PstI C_TGCA'G Cuts at: 830 2218 2540 830 Size: 1388 322 3478 Fragments arranged by size: 3478 1388 322 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5188 SalI G'TCGA_C Cuts at: 1936 2530 1936 Size: 594 4594 SbfI CC_TGCA'GG Cuts at: 2540 2540 Size: 5188 ScaI AGT'ACT Cuts at: 3436 5148 3436 Size: 1712 3476 SfcI C'TryA_G Cuts at: 826 1045 2017 2214 2422 2536 3671 4352 4543 Size: 219 972 197 208 114 1135 681 191 Cuts at: 4543 826 Size: 1471 Fragments arranged by size: 1471 1135 972 681 219 208 197 191 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5188 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5188 SmaI CCC'GGG Cuts at: 1872 2515 1872 Size: 643 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3292 4193 4455 820 Size: 197 926 1349 901 262 1553 Fragments arranged by size: 1553 1349 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2979 493 Size: 2486 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5188 SphI G_CATG'C Cuts at: 1132 2546 1132 Size: 1414 3774 SspI AAT'ATT Cuts at: 5 52 3112 5 Size: 47 3060 2081 Fragments arranged by size: 3060 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5188 StyI C'CwwG_G Cuts at: 513 1065 2004 2627 513 Size: 552 939 623 3074 Fragments arranged by size: 3074 939 623 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2064 3215 3232 3385 3570 4015 325 Size: 1255 484 1151 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1151 484 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 2265 2470 Size: 274 80 33 51 1443 267 21 205 Cuts at: 2470 3434 5146 96 Size: 964 1712 138 Fragments arranged by size: 1712 1443 964 274 267 205 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5148 5180 5148 Size: 32 5156 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5188 XbaI T'CTAG_A Cuts at: 2524 2524 Size: 5188 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5188 XmaI C'CCGG_G Cuts at: 1870 2513 1870 Size: 643 4545 XmnI GAAnn'nnTTC Cuts at: 3317 3317 Size: 5188 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2996 276 Size: 53 83 186 936 1462 2468 Fragments arranged by size: 2468 1462 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI