Restriction map for
pFN21AE3117
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
Aor51HI ApoI AscI AseI AsnI
Asp700I AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 155 GGTACC G^GTACC
AhdI 211 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 746 GTGCAC G^TGCAC
AocI 663 CCTGAGG CC^T[ACGT]AGG
ApaI 83 GGGCCC GGGCC^C
ApaLI 746 GTGCAC G^TGCAC
Asp718I 155 GGTACC G^GTACC
AspEI 211 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 96 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 449 CCCGAG C^[CT]CG[GA]G
AviII 182 TGCGCA TGC^GCA
BciVI 427 GGATAC GTATCC(6/5)
BcnI 274 CCCGG CC^[GC]GG
BglI 530 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 847 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 847 GCTCAGC GC^T[ACGT]AGC
BsaAI 253 TACGTG [CT]AC^GT[GA]
BsmAI 209 GAGAC GTCTC(1/5)
BsoBI 449 CCCGAG C^[CT]CG[GA]G
BstAPI 317 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 277 GGTGACC G^GT[ACGT]ACC
BstPI 277 GGTGACC G^GT[ACGT]ACC
BstYI 362 AGATCC [GA]^GATC[CT]
Bsu36I 663 CCTGAGG CC^T[ACGT]AGG
CelII 847 GCTCAGC GC^T[ACGT]AGC
CfoI 183 GCGC GCG^C
DraIII 550 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 10 CCATGG C^C[GA][CT]GG
Eam1105I 211 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 663 CCTGAGG CC^T[ACGT]AGG
EcoNI 349 CCTGTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 277 GGTGACC G^GT[ACGT]ACC
EcoT14I 10 CCATGG C^C[AT][AT]GG
FspI 182 TGCGCA TGC^GCA
HhaI 183 GCGC GCG^C
HinP1I 183 GCGC G^CGC
KpnI 155 GGTACC GGTAC^C
MflI 362 AGATCC [GA]^GATC[CT]
NaeI 535 GCCGGC GCC^GGC
NciI 274 CCCGG CC^[GC]GG
NcoI 10 CCATGG C^CATGG
NgoMIV 535 GCCGGC G^CCGGC
PflFI 96 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 280 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 673 CTGCAG CTGCA^G
SacI 559 GAGCTC GAGCT^C
SapI 735 GCTCTTC GCTCTTC(1/4)
ScaI 329 AGTACT AGT^ACT
SexAI 629 ACCTGGT A^CC[AT]GGT
SfaNI 369 GATGC GCATC(5/9)
SfcI 673 CTGCAG C^T[GA][CT]AG
SmlI 333 CTTGAG C^T[CT][GA]AG
StyI 10 CCATGG C^C[AT][AT]GG
TaqI 233 TCGA T^CGA
Tsp509I 410 AATT ^AATT
Tth111I 96 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 233 TCGA T^CGA
XhoII 362 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 156 GTAC GT^AC
330 GTAC
BanI 155 GGTACC G^G[CT][GA]CC
709 GGTGCC
BseRI 235 GAGGAG GAGGAG(10/8)
382 GAGGAG
BspMI 671 ACCTGC ACCTGC(4/8)
706 GCAGGT
BsrBI 238 GAGCGG CCGCTC(-3/-3)
487 GAGCGG
BsrDI 111 CATTGC GCAATG(2/0)
143 CATTGC
EarI 736 CTCTTC CTCTTC(1/4)
18 GAAGAG
HinfI 44 GACTC G^A[ACGT]TC
618 GAGTC
Ksp632I 736 CTCTTC CTCTTC(1/4)
18 GAAGAG
MaeII 254 ACGT A^CGT
584 ACGT
MboII 18 GAAGA GAAGA(8/7)
737 TCTTC
PleI 44 GACTC GAGTC(4/5)
618 GAGTC
PpuMI 491 GGGACCC [GA]G^G[AT]CC[CT]
668 GGGACCT
PvuII 613 CAGCTG CAG^CTG
676 CAGCTG
RsaI 156 GTAC GT^AC
330 GTAC
Tsp45I 278 GTGAC ^GT[GC]AC
287 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanII BbvI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeIII
MboI MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI TspRI