(Circular) (Six-base) MAPSORT of: pFN21AE3117.seq Check: 6561 from: 1 to: 5578 pFN21AE3117 5578 bp With 182 enzymes: SgfI * March 28, 2012 12:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2702 2702 Size: 5578 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3388 278 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2921 1937 Size: 984 4594 Acc65I G'GTAC_C Cuts at: 2160 2899 2160 Size: 739 4839 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2236 3641 5025 5293 1632 Size: 604 1405 1384 268 1917 Fragments arranged by size: 1917 1405 1384 604 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 2221 4307 2221 Size: 2086 3492 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2213 1184 Size: 1029 4549 AlwNI CAG_nnn'CTG Cuts at: 2045 2328 2371 2519 2686 3321 4893 2045 Size: 283 43 148 167 635 1572 2730 Fragments arranged by size: 2730 1572 635 283 167 148 43 ApaI G_GGCC'C Cuts at: 2092 2092 Size: 5578 ApaLI G'TGCA_C Cuts at: 2751 3635 4791 2751 Size: 884 1156 3538 Fragments arranged by size: 3538 1156 884 ApoI r'AATT_y Cuts at: 1493 2887 3113 3265 5374 1493 Size: 1394 226 152 2109 1697 Fragments arranged by size: 2109 1697 1394 226 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2454 2903 1870 Size: 73 511 449 4545 Fragments arranged by size: 4545 511 449 73 BamHI G'GATC_C Cuts at: 2908 3275 2908 Size: 367 5211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2160 2714 2899 4354 618 Size: 325 217 627 373 554 185 1455 1842 Fragments arranged by size: 1842 1455 627 554 373 325 217 185 BanII G_rGCy'C Cuts at: 1391 2092 2461 2568 2897 1391 Size: 701 369 107 329 4072 Fragments arranged by size: 4072 701 369 329 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3769 3803 1903 Size: 34 1832 34 3678 Fragments arranged by size: 3678 1832 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2426 3470 4680 2426 Size: 1044 1210 3324 Fragments arranged by size: 3324 1210 1044 BclI T'GATC_A Cuts at: 1548 1548 Size: 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2541 4189 136 Size: 107 122 71 631 605 869 1648 1525 Fragments arranged by size: 1648 1525 869 631 605 122 107 71 BglII A'GATC_T Cuts at: 5573 5573 Size: 5578 BlpI GC'TnA_GC Cuts at: 2853 2995 2853 Size: 142 5436 Bme1580I G_kGCm'C Cuts at: 1790 2092 2755 3639 4795 1790 Size: 302 663 884 1156 2573 Fragments arranged by size: 2573 1156 884 663 302 BmgBI CAC'GTC Cuts at: 1318 2590 1318 Size: 1272 4306 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2605 3309 4267 Size: 416 53 83 399 24 1188 704 958 Cuts at: 4267 442 Size: 1753 Fragments arranged by size: 1753 1188 958 704 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2338 2708 4238 1239 Size: 824 275 370 1530 2579 Fragments arranged by size: 2579 1530 824 370 275 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2359 3703 4568 4866 2359 Size: 1344 865 298 3071 Fragments arranged by size: 3071 1344 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4241 882 Size: 617 2742 2219 Fragments arranged by size: 2742 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2260 3369 493 Size: 711 1056 1109 2702 Fragments arranged by size: 2702 1109 1056 711 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3385 3767 4402 275 Size: 53 83 186 936 1852 382 635 1451 Fragments arranged by size: 1852 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4011 4409 4683 4830 5241 4011 Size: 398 274 147 411 4348 Fragments arranged by size: 4348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5353 Fragments arranged by size: 5353 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2402 1186 Size: 1069 147 4362 Fragments arranged by size: 4362 1069 147 BseYI C'CCAG_C Cuts at: 4781 5277 4781 Size: 496 5082 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5005 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3028 3789 4817 1540 Size: 275 186 1027 761 1028 2301 Fragments arranged by size: 2301 1028 1027 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2071 2265 2556 2568 2643 2742 2755 3639 3724 Size: 194 291 12 75 99 13 884 85 Cuts at: 3724 4795 2071 Size: 1071 2854 Fragments arranged by size: 2854 1071 884 291 194 99 85 75 13 12 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2092 2265 2461 2556 2568 2643 Size: 399 281 21 173 196 95 12 75 Cuts at: 2643 2742 2755 2897 3639 3724 4795 1391 Size: 99 13 142 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 281 196 173 142 99 95 85 75 21 13 12 BspEI T'CCGG_A Cuts at: 5241 5241 Size: 5578 BspHI T'CATG_A Cuts at: 3467 5197 3467 Size: 1730 3848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2685 2702 2933 844 Size: 1841 17 231 3489 Fragments arranged by size: 3489 1841 231 17 BsrBI CCG'CTC Cuts at: 1394 1823 2245 2494 3465 1394 Size: 429 422 249 971 3507 Fragments arranged by size: 3507 971 429 422 249 BsrDI GCAATG_nn' Cuts at: 65 1248 2115 2147 4073 4247 65 Size: 1183 867 32 1926 174 1396 Fragments arranged by size: 1926 1396 1183 867 174 32 BsrFI r'CCGG_y Cuts at: 1700 1810 2163 2540 2652 4222 4409 1700 Size: 110 353 377 112 1570 187 2869 Fragments arranged by size: 2869 1570 377 353 187 112 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5578 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5578 BssSI C'ACGA_G Cuts at: 3638 4650 3638 Size: 1012 4566 BstAPI GCAn_nnn'nTGC Cuts at: 794 2328 794 Size: 1534 4044 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 2282 1184 Size: 1098 4480 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2725 2813 2122 Size: 603 88 4887 Fragments arranged by size: 4887 603 88 BstYI r'GATC_y Cuts at: 1972 2367 2908 3275 3660 3677 5129 5215 5227 Size: 395 541 367 385 17 1452 86 12 Cuts at: 5227 5573 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 541 395 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2669 2669 Size: 5578 BtgI C'CryG_G Cuts at: 513 1065 2004 2015 513 Size: 552 939 11 4076 Fragments arranged by size: 4076 939 552 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4839 Fragments arranged by size: 4839 683 56 BtsI GCAGTG_nn' Cuts at: 2520 3094 3888 3908 2520 Size: 574 794 20 4190 Fragments arranged by size: 4190 794 574 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DraIII CAC_nnn'GTG Cuts at: 2560 2560 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4585 809 Size: 507 3269 1802 Fragments arranged by size: 3269 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2538 2654 2785 Size: 54 1425 50 275 18 708 116 131 Cuts at: 2785 3025 3914 5543 8 Size: 240 889 1629 43 Fragments arranged by size: 1629 1425 889 708 275 240 131 116 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3025 1537 Size: 275 1213 4090 Fragments arranged by size: 4090 1213 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2747 3508 1353 Size: 665 729 761 3423 Fragments arranged by size: 3423 761 729 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2522 4165 4539 4685 387 Size: 1064 138 933 1643 374 146 1280 Fragments arranged by size: 1643 1280 1064 933 374 146 138 EcoICRI GAG'CTC Cuts at: 2566 2566 Size: 5578 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2236 2338 2708 3641 4238 5025 Size: 393 431 173 102 370 933 597 787 Cuts at: 5025 5293 1239 Size: 268 1524 Fragments arranged by size: 1524 933 787 597 431 393 370 268 173 102 EcoNI CCTnn'n_nnAGG Cuts at: 2358 2358 Size: 5578 EcoO109I rG'GnC_Cy Cuts at: 2042 2088 2351 2395 2497 2674 2042 Size: 46 263 44 102 177 4946 Fragments arranged by size: 4946 263 177 102 46 44 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FspI TGC'GCA Cuts at: 2189 4084 2189 Size: 1895 3683 FspAI rTGC'GCAy Cuts at: 2189 2189 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 4725 1678 Size: 3047 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 4219 4251 4293 Size: 32 135 32 635 32 1980 32 42 Cuts at: 4293 4325 1373 Size: 32 2626 Fragments arranged by size: 2626 1980 635 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2922 3168 669 Size: 988 281 984 246 3079 Fragments arranged by size: 3079 988 984 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KpnI G_GTAC'C Cuts at: 2164 2903 2164 Size: 739 4839 MfeI C'AATT_G Cuts at: 3177 3177 Size: 5578 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4692 4876 1305 Size: 3387 184 2007 Fragments arranged by size: 3387 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3756 Fragments arranged by size: 3756 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3536 3895 4054 518 Size: 648 2370 359 159 2042 Fragments arranged by size: 2370 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2328 2620 2683 2796 2994 3671 4819 5064 Size: 722 292 63 113 198 677 1148 245 Cuts at: 5064 1606 Size: 2120 Fragments arranged by size: 2120 1148 722 677 292 245 198 113 63 NaeI GCC'GGC Cuts at: 2542 2542 Size: 5578 NcoI C'CATG_G Cuts at: 513 1065 2004 2015 513 Size: 552 939 11 4076 Fragments arranged by size: 4076 939 552 11 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NgoMIV G'CCGG_C Cuts at: 2540 2540 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2936 4481 1132 Size: 97 1707 1545 2229 Fragments arranged by size: 2229 1707 1545 97 PasI CC'CwG_GG Cuts at: 1348 2085 2468 1348 Size: 737 383 4458 Fragments arranged by size: 4458 737 383 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2497 2674 2497 Size: 177 5401 PshAI GACnn'nnGTC Cuts at: 1613 2289 1613 Size: 676 4902 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PspOMI G'GGCC_C Cuts at: 2088 2088 Size: 5578 PstI C_TGCA'G Cuts at: 830 2682 2930 830 Size: 1852 248 3478 Fragments arranged by size: 3478 1852 248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 PvuII CAG'CTG Cuts at: 2620 2683 2620 Size: 63 5515 SacI G_AGCT'C Cuts at: 2568 2568 Size: 5578 SalI G'TCGA_C Cuts at: 1936 2920 1936 Size: 984 4594 SanDI GG'GwC_CC Cuts at: 2497 2497 Size: 5578 SapI GCTCTTCn'nnn_ Cuts at: 2747 2747 Size: 5578 SbfI CC_TGCA'GG Cuts at: 2930 2930 Size: 5578 ScaI AGT'ACT Cuts at: 2336 3826 5538 2336 Size: 1490 1712 2376 Fragments arranged by size: 2376 1712 1490 SexAI A'CCwGG_T Cuts at: 2634 2634 Size: 5578 SfcI C'TryA_G Cuts at: 826 1045 2678 2926 4061 4742 4933 826 Size: 219 1633 248 1135 681 191 1471 Fragments arranged by size: 1633 1471 1135 681 248 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2905 1872 Size: 1033 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 3682 4583 4845 820 Size: 197 926 395 1344 901 262 1553 Fragments arranged by size: 1553 1344 926 901 395 262 197 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2936 1132 Size: 1804 3774 SspI AAT'ATT Cuts at: 5 52 3502 5 Size: 47 3450 2081 Fragments arranged by size: 3450 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5578 StyI C'CwwG_G Cuts at: 513 1065 2004 2015 3017 513 Size: 552 939 11 1002 3074 Fragments arranged by size: 3074 1002 939 552 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 2624 3605 3622 3775 3960 4405 Size: 1255 702 342 981 17 153 185 445 Cuts at: 4405 325 Size: 1498 Fragments arranged by size: 1498 1255 981 702 445 342 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2334 3824 5536 Size: 274 80 33 51 1443 357 1490 1712 Cuts at: 5536 96 Size: 138 Fragments arranged by size: 1712 1490 1443 357 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2803 2835 5538 5570 2803 Size: 32 2703 32 2811 Fragments arranged by size: 2811 2703 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2104 1316 Size: 788 4790 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2903 1870 Size: 1033 4545 XmnI GAAnn'nnTTC Cuts at: 3707 3707 Size: 5578 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3386 276 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI