##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MboI        
MboII       MfeI        MflI        MluI        MroI        
MseI        MslI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AciI          270  CCGC                 CCGC(-3/-1)
AfaI          380  GTAC                 GT^AC
BalI          211  TGGCCA               TGG^CCA
BcnI          360  CCGGG                CC^[GC]GG
BsgI          427  CTGCAC               GTGCAG(16/14)
BsrDI         305  GCAATG               GCAATG(2/0)
BssSI         454  CTCGTG               CACGAG(-5/-1)
BstF5I        288  CATCC                GGATG(2/0)
Cac8I         262  GCCTGC               GC[ACGT]^[ACGT]GC
EaeI          211  TGGCCA               [CT]^GGCC[GA]
EcoT14I       398  CCTTGG               C^C[AT][AT]GG
FokI          288  CATCC                GGATG(9/13)
HapII         360  CCGG                 C^CGG
HgaI          480  GCGTC                GACGC(5/10)
HpaII         360  CCGG                 C^CGG
MluNI         211  TGGCCA               TGG^CCA
MscI          211  TGGCCA               TGG^CCA
MspI          360  CCGG                 C^CGG
NciI          360  CCGGG                CC^[GC]GG
NlaIII          6  CATG                 CATG^
PflMI         229  CCACTGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          264  CTGCAG               CTGCA^G
RsaI          380  GTAC                 GT^AC
SfaNI         287  GCATC                GCATC(5/9)
SfcI          264  CTGCAG               C^T[GA][CT]AG
StuI          395  AGGCCT               AGG^CCT
StyI          398  CCTTGG               C^C[AT][AT]GG
Van91I        229  CCACTGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BbvCI          30  CCTCAGC              CCTCAGC(-5/-2)
              513  GCTGAGG             
BlpI          181  GCTAAGC              GC^T[ACGT]AGC
              243  GCTGAGC             
BpmI          552  CTCCAG               CTGGAG(16/14)
              406  CTGGAG              
Bpu1102I      181  GCTAAGC              GC^T[ACGT]AGC
              243  GCTGAGC             
BsaJI         330  CCCAGG               C^C[ACGT][ACGT]GG
              398  CCTTGG              
BsmAI           9  GAGAC                GTCTC(1/5)
               97  GTCTC               
CelII         181  GCTAAGC              GC^T[ACGT]AGC
              243  GCTGAGC             
DraII          44  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              143  AGGACCC             
EcoO109I       44  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              143  AGGACCC             
MwoI          115  GCTGCCACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              405  GCTGGAGCAGC         
PpuMI          44  GGGTCCT              [GA]G^G[AT]CC[CT]
              143  AGGACCC             
XcmI          331  CCAGGCCGACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              498  CCAGCTCTGGGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AluI        AspHI       AvaII       BbvI        BmyI        
BsiHKAI     BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstNI       Cfr13I      DdeI        EcoRII      
Fnu4HI      HaeIII      ItaI        MaeII       MaeIII      
MnlI        MspA1I      MvaI        NlaIV       PvuII       
Sau96I      ScrFI       TseI        Tsp45I      TspRI