(Circular) (Six-base) MAPSORT of: pFN21AB3260.seq Check: 5614 from: 1 to: 9550 pFN21AB3260 9550 bp With 182 enzymes: SgfI * January 7, 2013 17:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3097 5786 3097 Size: 2689 6861 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3744 4086 7360 278 Size: 53 83 186 936 2208 342 3274 2468 Fragments arranged by size: 3274 2468 2208 936 342 186 83 53 AccI GT'mk_AC Cuts at: 1937 3165 3273 5361 6893 1937 Size: 1228 108 2088 1532 4594 Fragments arranged by size: 4594 2088 1532 1228 108 Acc65I G'GTAC_C Cuts at: 5241 6871 5241 Size: 1630 7920 AclI AA'CG_TT Cuts at: 1563 7677 8050 1563 Size: 6114 373 3063 Fragments arranged by size: 6114 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4112 5423 7613 8997 9265 1632 Size: 2480 1311 2190 1384 268 1917 Fragments arranged by size: 2480 2190 1917 1384 1311 268 AfeI AGC'GCT Cuts at: 6245 6245 Size: 9550 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9353 AflIII A'CryG_T Cuts at: 1203 2496 3008 6546 6642 8449 9426 1203 Size: 1293 512 3538 96 1807 977 1327 Fragments arranged by size: 3538 1807 1327 1293 977 512 96 AgeI A'CCGG_T Cuts at: 8381 8381 Size: 9550 AhdI GACnn_n'nnGTC Cuts at: 8279 8279 Size: 9550 AleI CACnn'nnGTG Cuts at: 1166 2174 2981 5652 5713 6156 6641 1166 Size: 1008 807 2671 61 443 485 4075 Fragments arranged by size: 4075 2671 1008 807 485 443 61 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3147 1184 Size: 1963 7587 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2669 2701 4238 4270 2669 Size: 32 1537 32 7949 Fragments arranged by size: 7949 1537 32 32 AlwNI CAG_nnn'CTG Cuts at: 2863 3232 5476 5752 6672 6754 7293 8865 2863 Size: 369 2244 276 920 82 539 1572 3548 Fragments arranged by size: 3548 2244 1572 920 539 369 276 82 ApaI G_GGCC'C Cuts at: 6171 6267 6171 Size: 96 9454 ApaLI G'TGCA_C Cuts at: 3716 7607 8763 3716 Size: 3891 1156 4503 Fragments arranged by size: 4503 3891 1156 ApoI r'AATT_y Cuts at: 1493 6859 7085 7237 9346 1493 Size: 5366 226 152 2109 1697 Fragments arranged by size: 5366 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8104 160 Size: 872 7072 1606 Fragments arranged by size: 7072 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9550 AvaI C'yCGr_G Cuts at: 1870 1943 3387 3509 3983 4163 4291 5828 5855 Size: 73 1444 122 474 180 128 1537 27 Cuts at: 5855 6322 6875 1870 Size: 467 553 4545 Fragments arranged by size: 4545 1537 1444 553 474 467 180 128 122 73 27 BamHI G'GATC_C Cuts at: 4309 6880 7247 4309 Size: 2571 367 6612 Fragments arranged by size: 6612 2571 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3344 4992 5241 5303 6412 Size: 325 217 627 1557 1648 249 62 1109 Cuts at: 6412 6613 6871 8326 618 Size: 201 258 1455 1842 Fragments arranged by size: 1842 1648 1557 1455 1109 627 325 258 249 217 201 62 BanII G_rGCy'C Cuts at: 1391 3387 3777 4127 6108 6171 6267 6383 6869 Size: 1996 390 350 1981 63 96 116 486 Cuts at: 6869 1391 Size: 4072 Fragments arranged by size: 4072 1996 1981 486 390 350 116 96 63 BbeI G_GCGC'C Cuts at: 4996 6617 4996 Size: 1621 7929 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3923 3940 4100 5555 928 Size: 971 2024 17 160 1455 4923 Fragments arranged by size: 4923 2024 1455 971 160 17 BbvCI CC'TCA_GC Cuts at: 4036 5090 4036 Size: 1054 8496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3253 3287 4345 4379 6145 6179 7741 Size: 34 1316 34 1058 34 1766 34 1562 Cuts at: 7741 7775 1903 Size: 34 3678 Fragments arranged by size: 3678 1766 1562 1316 1058 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5834 7442 8652 5834 Size: 1608 1210 6732 Fragments arranged by size: 6732 1608 1210 BclI T'GATC_A Cuts at: 1548 3591 1548 Size: 2043 7507 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3342 3891 Size: 107 122 71 631 605 337 1333 549 Cuts at: 3891 4707 8161 136 Size: 816 3454 1525 Fragments arranged by size: 3454 1525 1333 816 631 605 549 337 122 107 71 BglII A'GATC_T Cuts at: 9545 9545 Size: 9550 BlpI GC'TnA_GC Cuts at: 2651 2864 5162 6967 2651 Size: 213 2298 1805 5234 Fragments arranged by size: 5234 2298 1805 213 Bme1580I G_kGCm'C Cuts at: 1790 2836 3314 3720 5659 6171 6267 6306 6417 Size: 1046 478 406 1939 512 96 39 111 Cuts at: 6417 7611 8767 1790 Size: 1194 1156 2573 Fragments arranged by size: 2573 1939 1194 1156 1046 512 478 406 111 96 39 BmgBI CAC'GTC Cuts at: 1318 2248 3009 4003 5127 6436 6643 1318 Size: 930 761 994 1124 1309 207 4225 Fragments arranged by size: 4225 1309 1124 994 930 761 207 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2362 2521 2644 Size: 416 53 83 399 24 945 159 123 Cuts at: 2644 2814 3286 3445 3568 4213 4224 4990 5381 Size: 170 472 159 123 645 11 766 391 Cuts at: 5381 5720 5902 7281 8239 442 Size: 339 182 1379 958 1753 Fragments arranged by size: 1753 1379 958 945 766 645 472 416 399 391 339 182 170 159 159 123 123 83 53 24 11 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9550 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5656 5656 Size: 9550 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2210 2636 3647 3917 5471 5651 8210 Size: 944 27 426 1011 270 1554 180 2559 Cuts at: 8210 1239 Size: 2579 Fragments arranged by size: 2579 2559 1554 1011 944 426 270 180 27 Bpu10I CC'TnA_GC Cuts at: 4036 5090 4036 Size: 1054 8496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7675 8540 8838 7675 Size: 865 298 8387 Fragments arranged by size: 8387 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2332 2381 3980 6574 8213 882 Size: 617 833 49 1599 2594 1639 2219 Fragments arranged by size: 2594 2219 1639 1599 833 617 49 BsaAI yAC'GTr Cuts at: 493 1204 4138 6301 7341 493 Size: 711 2934 2163 1040 2702 Fragments arranged by size: 2934 2702 2163 1040 711 BsaBI GATnn'nnATC Cuts at: 4676 9544 4676 Size: 4868 4682 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 2522 2643 3567 Size: 53 83 186 936 612 377 121 924 Cuts at: 3567 3741 4023 4083 4993 5457 6003 6614 6626 Size: 174 282 60 910 464 546 611 12 Cuts at: 6626 7357 7739 8374 275 Size: 731 382 635 1451 Fragments arranged by size: 1451 936 924 910 731 635 612 611 546 464 382 377 282 186 174 121 83 60 53 12 BsaWI w'CCGG_w Cuts at: 6027 6426 7983 8381 8655 8802 9213 6027 Size: 399 1557 398 274 147 411 6364 Fragments arranged by size: 6364 1557 411 399 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3332 3362 3962 3992 5225 Size: 30 165 30 1929 30 600 30 1233 Cuts at: 5225 5255 1178 Size: 30 5473 Fragments arranged by size: 5473 1929 1233 600 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2633 2918 3041 4684 5648 5894 6811 Size: 1084 363 285 123 1643 964 246 917 Cuts at: 6811 1186 Size: 3925 Fragments arranged by size: 3925 1643 1084 964 917 363 285 246 123 BseYI C'CCAG_C Cuts at: 3664 4504 4811 5028 6333 8753 9249 3664 Size: 840 307 217 1305 2420 496 3965 Fragments arranged by size: 3965 2420 1305 840 496 307 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2321 3005 3386 3859 4583 5489 6820 Size: 573 585 684 381 473 724 906 1331 Cuts at: 6820 1163 Size: 3893 Fragments arranged by size: 3893 1331 906 724 684 585 573 473 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2202 3273 4633 5518 6427 7000 Size: 275 186 201 1071 1360 885 909 573 Cuts at: 7000 7761 8789 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1360 1071 1028 909 885 761 573 275 201 186 BsiHKAI G_wGCw'C Cuts at: 2439 3720 4127 4137 5720 5742 5900 6108 6383 Size: 1281 407 10 1583 22 158 208 275 Cuts at: 6383 7611 7696 8767 2439 Size: 1228 85 1071 3222 Fragments arranged by size: 3222 1583 1281 1228 1071 407 275 208 158 85 22 10 BsiWI C'GTAC_G Cuts at: 2131 2131 Size: 9550 BsmI GAATG_Cn' Cuts at: 7061 7154 7061 Size: 93 9457 BsmBI CGTCTCn'nnnn_ Cuts at: 2513 3558 2513 Size: 1045 8505 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2439 2836 3314 3387 3720 3777 4127 Size: 399 649 397 478 73 333 57 350 Cuts at: 4127 4137 5659 5720 5742 5900 6108 6171 6267 Size: 10 1522 61 22 158 208 63 96 Cuts at: 6267 6306 6383 6417 6869 7611 7696 8767 1391 Size: 39 77 34 452 742 85 1071 2174 Fragments arranged by size: 2174 1522 1071 742 649 478 452 399 397 350 333 208 158 96 85 77 73 63 61 57 39 34 22 10 BspEI T'CCGG_A Cuts at: 9213 9213 Size: 9550 BspHI T'CATG_A Cuts at: 4113 7439 9169 4113 Size: 3326 1730 4494 Fragments arranged by size: 4494 3326 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3097 3546 3929 5760 5786 5949 6905 844 Size: 2253 449 383 1831 26 163 956 3489 Fragments arranged by size: 3489 2253 1831 956 449 383 163 26 BsrBI CCG'CTC Cuts at: 1394 1823 2104 2486 2536 2778 2875 3484 3712 Size: 429 281 382 50 242 97 609 228 Cuts at: 3712 4015 4516 4612 5166 5982 6039 7437 1394 Size: 303 501 96 554 816 57 1398 3507 Fragments arranged by size: 3507 1398 816 609 554 501 429 382 303 281 242 228 97 96 57 50 BsrDI GCAATG_nn' Cuts at: 65 1248 3129 8045 8219 65 Size: 1183 1881 4916 174 1396 Fragments arranged by size: 4916 1881 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 5330 8194 8381 1700 Size: 110 337 3183 2864 187 2869 Fragments arranged by size: 3183 2869 2864 337 187 110 BsrGI T'GTAC_A Cuts at: 96 6534 96 Size: 6438 3112 BssHII G'CGCG_C Cuts at: 1925 5919 1925 Size: 3994 5556 BssSI C'ACGA_G Cuts at: 4268 5735 6275 7610 8622 4268 Size: 1467 540 1335 1012 5196 Fragments arranged by size: 5196 1467 1335 1012 540 BstAPI GCAn_nnn'nTGC Cuts at: 794 5752 6360 794 Size: 4958 608 3984 Fragments arranged by size: 4958 3984 608 BstBI TT'CG_AA Cuts at: 8354 8354 Size: 9550 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9550 BstXI CCAn_nnnn'nTGG Cuts at: 2176 2476 2614 3526 3895 4504 5182 5600 2176 Size: 300 138 912 369 609 678 418 6126 Fragments arranged by size: 6126 912 678 609 418 369 300 138 BstYI r'GATC_y Cuts at: 1972 2823 3066 3357 3975 4309 4906 6880 7247 Size: 851 243 291 618 334 597 1974 367 Cuts at: 7247 7632 7649 9101 9187 9199 9545 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1974 1452 851 618 597 385 367 346 334 291 243 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3933 3933 Size: 9550 BtgI C'CryG_G Cuts at: 513 1065 2004 2030 2091 2847 2976 5175 5711 Size: 552 939 26 61 756 129 2199 536 Cuts at: 5711 6519 513 Size: 808 3544 Fragments arranged by size: 3544 2199 939 808 756 552 536 129 61 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2455 3250 3379 5877 6340 487 Size: 683 56 1229 795 129 2498 463 3697 Fragments arranged by size: 3697 2498 1229 795 683 463 129 56 BtsI GCAGTG_nn' Cuts at: 2211 3398 4745 5619 6071 6163 6573 7066 7860 Size: 1187 1347 874 452 92 410 493 794 Cuts at: 7860 7880 2211 Size: 20 3881 Fragments arranged by size: 3881 1347 1187 874 794 493 452 410 92 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9550 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9515 DraI TTT'AAA Cuts at: 6854 6854 Size: 9550 DraIII CAC_nnn'GTG Cuts at: 3409 4141 5737 3409 Size: 732 1596 7222 Fragments arranged by size: 7222 1596 732 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8557 809 Size: 507 7241 1802 Fragments arranged by size: 7241 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2033 2199 2406 Size: 54 1425 50 275 18 203 166 207 Cuts at: 2406 3792 4005 4173 4630 4771 6040 6516 6570 Size: 1386 213 168 457 141 1269 476 54 Cuts at: 6570 6997 7886 9515 8 Size: 427 889 1629 43 Fragments arranged by size: 1629 1425 1386 1269 889 476 457 427 275 213 207 203 168 166 141 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2199 4630 6997 1537 Size: 275 387 2431 2367 4090 Fragments arranged by size: 4090 2431 2367 387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 2807 3605 5327 6200 6562 6818 7480 Size: 1091 363 798 1722 873 362 256 662 Cuts at: 7480 1353 Size: 3423 Fragments arranged by size: 3423 1722 1091 873 798 662 363 362 256 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2166 3064 3631 5330 5890 6008 Size: 1064 138 577 898 567 1699 560 118 Cuts at: 6008 6776 8137 8511 8657 387 Size: 768 1361 374 146 1280 Fragments arranged by size: 1699 1361 1280 1064 898 768 577 567 560 374 146 138 118 EcoICRI GAG'CTC Cuts at: 4125 6106 6381 4125 Size: 1981 275 7294 Fragments arranged by size: 7294 1981 275 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2210 2636 3647 3917 4112 5423 Size: 393 551 27 426 1011 270 195 1311 Cuts at: 5423 5471 5651 7613 8210 8997 9265 1239 Size: 48 180 1962 597 787 268 1524 Fragments arranged by size: 1962 1524 1311 1011 787 597 551 426 393 270 268 195 180 48 27 EcoNI CCTnn'n_nnAGG Cuts at: 3542 3626 4649 3542 Size: 84 1023 8443 Fragments arranged by size: 8443 1023 84 EcoO109I rG'GnC_Cy Cuts at: 2449 3373 5025 5317 5832 6264 6291 6444 6591 Size: 924 1652 292 515 432 27 153 147 Cuts at: 6591 2449 Size: 5408 Fragments arranged by size: 5408 1652 924 515 432 292 153 147 27 EcoRI G'AATT_C Cuts at: 6859 7237 6859 Size: 378 9172 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2455 2671 2970 2455 Size: 216 299 9035 Fragments arranged by size: 9035 299 216 FspI TGC'GCA Cuts at: 8056 8056 Size: 9550 HaeII r_GCGC'y Cuts at: 1678 2018 2069 3700 4456 4996 5842 6247 6617 Size: 340 51 1631 756 540 846 405 370 Cuts at: 6617 6666 8697 1678 Size: 49 2031 2531 Fragments arranged by size: 2531 2031 1631 846 756 540 405 370 340 51 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2592 2624 3951 3983 6689 Size: 32 135 32 1020 32 1327 32 2706 Cuts at: 6689 6721 8191 8223 8265 8297 1373 Size: 32 1470 32 42 32 2626 Fragments arranged by size: 2706 2626 1470 1327 1020 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3238 3274 4180 5227 6894 7140 Size: 988 281 1300 36 906 1047 1667 246 Cuts at: 7140 669 Size: 3079 Fragments arranged by size: 3079 1667 1300 1047 988 906 281 246 36 HindIII A'AGCT_T Cuts at: 748 748 Size: 9550 HpaI GTT'AAC Cuts at: 7140 7140 Size: 9550 KasI G'GCGC_C Cuts at: 4992 6613 4992 Size: 1621 7929 KpnI G_GTAC'C Cuts at: 5245 6875 5245 Size: 1630 7920 MfeI C'AATT_G Cuts at: 7149 7149 Size: 9550 MluI A'CGCG_T Cuts at: 9426 9426 Size: 9550 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3348 3784 4083 4521 4856 6123 8664 8848 Size: 2043 436 299 438 335 1267 2541 184 Cuts at: 8848 1305 Size: 2007 Fragments arranged by size: 2541 2043 2007 1267 438 436 335 299 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 3794 4007 10 Size: 54 1425 343 576 1386 213 5553 Fragments arranged by size: 5553 1425 1386 576 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2402 2858 2981 3326 4481 4682 Size: 648 1008 228 456 123 345 1155 201 Cuts at: 4682 5180 5258 5652 5713 6156 6279 6545 6641 Size: 498 78 394 61 443 123 266 96 Cuts at: 6641 7508 7867 8026 518 Size: 867 359 159 2042 Fragments arranged by size: 2042 1155 1008 867 648 498 456 443 394 359 345 266 228 201 159 123 123 96 78 61 MspA1I CmG'CkG Cuts at: 1606 2038 2093 2184 2989 3757 4750 4779 6288 Size: 432 55 91 805 768 993 29 1509 Cuts at: 6288 6672 6771 6966 7643 8791 9036 1606 Size: 384 99 195 677 1148 245 2120 Fragments arranged by size: 2120 1509 1148 993 805 768 677 432 384 245 195 99 91 55 29 NaeI GCC'GGC Cuts at: 2149 2149 Size: 9550 NarI GG'CG_CC Cuts at: 4993 6614 4993 Size: 1621 7929 NcoI C'CATG_G Cuts at: 513 1065 2004 2847 5175 513 Size: 552 939 843 2328 4888 Fragments arranged by size: 4888 2328 939 843 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9550 NgoMIV G'CCGG_C Cuts at: 2147 2147 Size: 9550 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9550 NotI GC'GGCC_GC Cuts at: 2199 6997 2199 Size: 4798 4752 NspI r_CATG'y Cuts at: 1132 1229 2500 2967 4504 5263 5802 6363 6550 Size: 97 1271 467 1537 759 539 561 187 Cuts at: 6550 6908 8453 1132 Size: 358 1545 2229 Fragments arranged by size: 2229 1545 1537 1271 759 561 539 467 358 187 97 PasI CC'CwG_GG Cuts at: 1348 5211 1348 Size: 3863 5687 PciI A'CATG_T Cuts at: 2496 6546 8449 2496 Size: 4050 1903 3597 Fragments arranged by size: 4050 3597 1903 PflMI CCAn_nnn'nTGG Cuts at: 1108 5614 6232 6672 1108 Size: 4506 618 440 3986 Fragments arranged by size: 4506 3986 618 440 PfoI T'CCnGG_A Cuts at: 2426 3624 4884 2426 Size: 1198 1260 7092 Fragments arranged by size: 7092 1260 1198 PmeI GTTT'AAAC Cuts at: 6854 6854 Size: 9550 PmlI CAC'GTG Cuts at: 4138 6301 4138 Size: 2163 7387 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 6695 6727 7619 7651 9132 9164 4109 Size: 32 2554 32 892 32 1481 32 4495 Fragments arranged by size: 4495 2554 1481 892 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2449 3373 5025 5317 6291 2449 Size: 924 1652 292 974 5708 Fragments arranged by size: 5708 1652 974 924 292 PshAI GACnn'nnGTC Cuts at: 1613 3233 4082 1613 Size: 1620 849 7081 Fragments arranged by size: 7081 1620 849 PsiI TTA'TAA Cuts at: 7120 7120 Size: 9550 PspOMI G'GGCC_C Cuts at: 6167 6263 6167 Size: 96 9454 PstI C_TGCA'G Cuts at: 830 2994 3108 3543 4829 5748 6635 6902 830 Size: 2164 114 435 1286 919 887 267 3478 Fragments arranged by size: 3478 2164 1286 919 887 435 267 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9550 PvuII CAG'CTG Cuts at: 2989 3757 4750 6771 2989 Size: 768 993 2021 5768 Fragments arranged by size: 5768 2021 993 768 RsrII CG'GwC_CG Cuts at: 2024 3316 2024 Size: 1292 8258 SacI G_AGCT'C Cuts at: 4127 6108 6383 4127 Size: 1981 275 7294 Fragments arranged by size: 7294 1981 275 SacII CC_GC'GG Cuts at: 2094 2094 Size: 9550 SalI G'TCGA_C Cuts at: 1936 3272 6892 1936 Size: 1336 3620 4594 Fragments arranged by size: 4594 3620 1336 SanDI GG'GwC_CC Cuts at: 5025 6291 5025 Size: 1266 8284 SapI GCTCTTCn'nnn_ Cuts at: 2444 2444 Size: 9550 SbfI CC_TGCA'GG Cuts at: 3543 6902 3543 Size: 3359 6191 ScaI AGT'ACT Cuts at: 6689 7798 9510 6689 Size: 1109 1712 6729 Fragments arranged by size: 6729 1712 1109 SfcI C'TryA_G Cuts at: 826 1045 2990 3104 3539 4825 5104 5744 6631 Size: 219 1945 114 435 1286 279 640 887 Cuts at: 6631 6658 6898 8033 8714 8905 826 Size: 27 240 1135 681 191 1471 Fragments arranged by size: 1945 1471 1286 1135 887 681 640 435 279 240 219 191 114 27 SfoI GGC'GCC Cuts at: 4994 6615 4994 Size: 1621 7929 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9550 SgrAI Cr'CCGG_yG Cuts at: 1700 2147 1700 Size: 447 9103 SmaI CCC'GGG Cuts at: 1872 5830 6324 6877 1872 Size: 3958 494 553 4545 Fragments arranged by size: 4545 3958 553 494 SmlI C'TyrA_G Cuts at: 820 1017 1943 3983 7654 8555 8817 820 Size: 197 926 2040 3671 901 262 1553 Fragments arranged by size: 3671 2040 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7341 493 Size: 6848 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9550 SphI G_CATG'C Cuts at: 1132 6363 6908 1132 Size: 5231 545 3774 Fragments arranged by size: 5231 3774 545 SspI AAT'ATT Cuts at: 5 52 7474 5 Size: 47 7422 2081 Fragments arranged by size: 7422 2081 47 StuI AGG'CCT Cuts at: 1517 3938 4034 1517 Size: 2421 96 7033 Fragments arranged by size: 7033 2421 96 StyI C'CwwG_G Cuts at: 513 1065 2004 2223 2475 2847 3795 4317 5013 Size: 552 939 219 252 372 948 522 696 Cuts at: 5013 5056 5175 5491 5694 6989 513 Size: 43 119 316 203 1295 3074 Fragments arranged by size: 3074 1295 948 939 696 552 522 372 316 252 219 203 119 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2344 2860 3268 3317 4352 4481 4541 Size: 1255 764 516 408 49 1035 129 60 Cuts at: 4541 4972 5377 5664 6194 6748 7577 7594 7747 Size: 431 405 287 530 554 829 17 153 Cuts at: 7747 7932 8377 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1035 829 764 554 530 516 445 431 408 405 287 185 153 129 60 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 2370 2499 Size: 274 80 33 51 1443 258 135 129 Cuts at: 2499 2754 3423 4563 5247 6534 6687 7796 9508 Size: 255 669 1140 684 1287 153 1109 1712 Cuts at: 9508 96 Size: 138 Fragments arranged by size: 1712 1443 1287 1140 1109 684 669 274 258 255 153 138 135 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3962 3994 9510 9542 3962 Size: 32 5516 32 3970 Fragments arranged by size: 5516 3970 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9550 XbaI T'CTAG_A Cuts at: 6886 6886 Size: 9550 XhoI C'TCGA_G Cuts at: 1943 3983 1943 Size: 2040 7510 XmaI C'CCGG_G Cuts at: 1870 5828 6322 6875 1870 Size: 3958 494 553 4545 Fragments arranged by size: 4545 3958 553 494 XmnI GAAnn'nnTTC Cuts at: 7679 7679 Size: 9550 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3742 4084 7358 276 Size: 53 83 186 936 2208 342 3274 2468 Fragments arranged by size: 3274 2468 2208 936 342 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI SexAI SfiI SrfI SwaI XcmI