(Linear) (Six-base) MAPSORT of: KIBB3260.seq Check: 6459 from: 1 to: 4845 KIBB3260.orf With 182 enzymes: SgfI * January 7, 2013 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1092 3781 4845 Size: 1092 2689 1064 Fragments arranged by size: 2689 1092 1064 AatII G_ACGT'C Cuts at: 0 1739 2081 4845 Size: 1739 342 2764 Fragments arranged by size: 2764 1739 342 AccI GT'mk_AC Cuts at: 0 1160 1268 3356 4845 Size: 1160 108 2088 1489 Fragments arranged by size: 2088 1489 1160 108 Acc65I G'GTAC_C Cuts at: 0 3236 4845 Size: 3236 1609 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2107 3418 4845 Size: 2107 1311 1427 Fragments arranged by size: 2107 1427 1311 AfeI AGC'GCT Cuts at: 0 4240 4845 Size: 4240 605 AflIII A'CryG_T Cuts at: 0 491 1003 4541 4637 4845 Size: 491 512 3538 96 208 Fragments arranged by size: 3538 512 491 208 96 AleI CACnn'nnGTG Cuts at: 0 169 976 3647 3708 4151 4636 4845 Size: 169 807 2671 61 443 485 209 Fragments arranged by size: 2671 807 485 443 209 169 61 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1142 4845 Size: 1142 3703 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 664 696 2233 2265 4845 Size: 664 32 1537 32 2580 Fragments arranged by size: 2580 1537 664 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 858 1227 3471 3747 4667 4749 4845 Size: 858 369 2244 276 920 82 96 Fragments arranged by size: 2244 920 858 369 276 96 82 ApaI G_GGCC'C Cuts at: 0 4166 4262 4845 Size: 4166 96 583 Fragments arranged by size: 4166 583 96 ApaLI G'TGCA_C Cuts at: 0 1711 4845 Size: 1711 3134 AvaI C'yCGr_G Cuts at: 0 1382 1504 1978 2158 2286 3823 3850 4317 Size: 1382 122 474 180 128 1537 27 467 Cuts at: 4317 4845 Size: 528 Fragments arranged by size: 1537 1382 528 474 467 180 128 122 27 BamHI G'GATC_C Cuts at: 0 2304 4845 Size: 2304 2541 BanI G'GyrC_C Cuts at: 0 1339 2987 3236 3298 4407 4608 4845 Size: 1339 1648 249 62 1109 201 237 Fragments arranged by size: 1648 1339 1109 249 237 201 62 BanII G_rGCy'C Cuts at: 0 1382 1772 2122 4103 4166 4262 4378 4845 Size: 1382 390 350 1981 63 96 116 467 Fragments arranged by size: 1981 1382 467 390 350 116 96 63 BbeI G_GCGC'C Cuts at: 0 2991 4612 4845 Size: 2991 1621 233 Fragments arranged by size: 2991 1621 233 BbsI GAAGACnn'nnnn_ Cuts at: 0 1918 1935 2095 3550 4845 Size: 1918 17 160 1455 1295 Fragments arranged by size: 1918 1455 1295 160 17 BbvCI CC'TCA_GC Cuts at: 0 2031 3085 4845 Size: 2031 1054 1760 Fragments arranged by size: 2031 1760 1054 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1248 1282 2340 2374 4140 4174 4845 Size: 1248 34 1058 34 1766 34 671 Fragments arranged by size: 1766 1248 1058 671 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 3829 4845 Size: 3829 1016 BclI T'GATC_A Cuts at: 0 1586 4845 Size: 1586 3259 BglI GCCn_nnn'nGGC Cuts at: 0 1337 1886 2702 4845 Size: 1337 549 816 2143 Fragments arranged by size: 2143 1337 816 549 BlpI GC'TnA_GC Cuts at: 0 646 859 3157 4845 Size: 646 213 2298 1688 Fragments arranged by size: 2298 1688 646 213 Bme1580I G_kGCm'C Cuts at: 0 831 1309 1715 3654 4166 4262 4301 4412 Size: 831 478 406 1939 512 96 39 111 Cuts at: 4412 4845 Size: 433 Fragments arranged by size: 1939 831 512 478 433 406 111 96 39 BmgBI CAC'GTC Cuts at: 0 243 1004 1998 3122 4431 4638 4845 Size: 243 761 994 1124 1309 207 207 Fragments arranged by size: 1309 1124 994 761 243 207 207 BmrI ACTGGGnnnn_n' Cuts at: 0 357 516 639 809 1281 1440 1563 2208 Size: 357 159 123 170 472 159 123 645 Cuts at: 2208 2219 2985 3376 3715 3897 4845 Size: 11 766 391 339 182 948 Fragments arranged by size: 948 766 645 472 391 357 339 182 170 159 159 123 123 11 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3651 4845 Size: 3651 1194 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 205 631 1642 1912 3466 3646 4845 Size: 178 27 426 1011 270 1554 180 1199 Fragments arranged by size: 1554 1199 1011 426 270 180 178 27 Bpu10I CC'TnA_GC Cuts at: 0 2031 3085 4845 Size: 2031 1054 1760 Fragments arranged by size: 2031 1760 1054 BsaI GGTCTCn'nnnn_ Cuts at: 0 327 376 1975 4569 4845 Size: 327 49 1599 2594 276 Fragments arranged by size: 2594 1599 327 276 49 BsaAI yAC'GTr Cuts at: 0 2133 4296 4845 Size: 2133 2163 549 Fragments arranged by size: 2163 2133 549 BsaBI GATnn'nnATC Cuts at: 0 2671 4845 Size: 2671 2174 BsaHI Gr'CG_yC Cuts at: 0 140 517 638 1562 1736 2018 2078 2988 Size: 140 377 121 924 174 282 60 910 Cuts at: 2988 3452 3998 4609 4621 4845 Size: 464 546 611 12 224 Fragments arranged by size: 924 910 611 546 464 377 282 224 174 140 121 60 12 BsaWI w'CCGG_w Cuts at: 0 4022 4421 4845 Size: 4022 399 424 Fragments arranged by size: 4022 424 399 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1327 1357 1957 1987 3220 3250 4845 Size: 1327 30 600 30 1233 30 1595 Fragments arranged by size: 1595 1327 1233 600 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 265 628 913 1036 2679 3643 3889 4806 Size: 265 363 285 123 1643 964 246 917 Cuts at: 4806 4845 Size: 39 Fragments arranged by size: 1643 964 917 363 285 265 246 123 39 BseYI C'CCAG_C Cuts at: 0 1659 2499 2806 3023 4328 4845 Size: 1659 840 307 217 1305 517 Fragments arranged by size: 1659 1305 840 517 307 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 1000 1381 1854 2578 3484 4815 4845 Size: 316 684 381 473 724 906 1331 30 Fragments arranged by size: 1331 906 724 684 473 381 316 30 BsiEI CG_ry'CG Cuts at: 0 197 1268 2628 3513 4422 4845 Size: 197 1071 1360 885 909 423 Fragments arranged by size: 1360 1071 909 885 423 197 BsiHKAI G_wGCw'C Cuts at: 0 434 1715 2122 2132 3715 3737 3895 4103 Size: 434 1281 407 10 1583 22 158 208 Cuts at: 4103 4378 4845 Size: 275 467 Fragments arranged by size: 1583 1281 467 434 407 275 208 158 22 10 BsiWI C'GTAC_G Cuts at: 0 126 4845 Size: 126 4719 BsmBI CGTCTCn'nnnn_ Cuts at: 0 508 1553 4845 Size: 508 1045 3292 Fragments arranged by size: 3292 1045 508 Bsp1286I G_dGCh'C Cuts at: 0 434 831 1309 1382 1715 1772 2122 2132 Size: 434 397 478 73 333 57 350 10 Cuts at: 2132 3654 3715 3737 3895 4103 4166 4262 4301 Size: 1522 61 22 158 208 63 96 39 Cuts at: 4301 4378 4412 4845 Size: 77 34 433 Fragments arranged by size: 1522 478 434 433 397 350 333 208 158 96 77 73 63 61 57 39 34 22 10 BspHI T'CATG_A Cuts at: 0 2108 4845 Size: 2108 2737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1092 1541 1924 3755 3781 3944 4845 Size: 1092 449 383 1831 26 163 901 Fragments arranged by size: 1831 1092 901 449 383 163 26 BsrBI CCG'CTC Cuts at: 0 99 481 531 773 870 1479 1707 2010 Size: 99 382 50 242 97 609 228 303 Cuts at: 2010 2511 2607 3161 3977 4034 4845 Size: 501 96 554 816 57 811 Fragments arranged by size: 816 811 609 554 501 382 303 242 228 99 97 96 57 50 BsrDI GCAATG_nn' Cuts at: 0 1124 4845 Size: 1124 3721 BsrFI r'CCGG_y Cuts at: 0 142 3325 4845 Size: 142 3183 1520 Fragments arranged by size: 3183 1520 142 BsrGI T'GTAC_A Cuts at: 0 4529 4845 Size: 4529 316 BssHII G'CGCG_C Cuts at: 0 3914 4845 Size: 3914 931 BssSI C'ACGA_G Cuts at: 0 2263 3730 4270 4845 Size: 2263 1467 540 575 Fragments arranged by size: 2263 1467 575 540 BstAPI GCAn_nnn'nTGC Cuts at: 0 3747 4355 4845 Size: 3747 608 490 Fragments arranged by size: 3747 608 490 BstXI CCAn_nnnn'nTGG Cuts at: 0 171 471 609 1521 1890 2499 3177 3595 Size: 171 300 138 912 369 609 678 418 Cuts at: 3595 4845 Size: 1250 Fragments arranged by size: 1250 912 678 609 418 369 300 171 138 BstYI r'GATC_y Cuts at: 0 818 1061 1352 1970 2304 2901 4845 Size: 818 243 291 618 334 597 1944 Fragments arranged by size: 1944 818 618 597 334 291 243 Bsu36I CC'TnA_GG Cuts at: 0 1928 4845 Size: 1928 2917 BtgI C'CryG_G Cuts at: 0 25 86 842 971 3170 3706 4514 4845 Size: 25 61 756 129 2199 536 808 331 Fragments arranged by size: 2199 808 756 536 331 129 61 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 450 1245 1374 3872 4335 4845 Size: 450 795 129 2498 463 510 Fragments arranged by size: 2498 795 510 463 450 129 BtsI GCAGTG_nn' Cuts at: 0 206 1393 2740 3614 4066 4158 4568 4845 Size: 206 1187 1347 874 452 92 410 277 Fragments arranged by size: 1347 1187 874 452 410 277 206 92 DraIII CAC_nnn'GTG Cuts at: 0 1404 2136 3732 4845 Size: 1404 732 1596 1113 Fragments arranged by size: 1596 1404 1113 732 EaeI y'GGCC_r Cuts at: 0 28 194 401 1787 2000 2168 2625 2766 Size: 28 166 207 1386 213 168 457 141 Cuts at: 2766 4035 4511 4565 4845 Size: 1269 476 54 280 Fragments arranged by size: 1386 1269 476 457 280 213 207 168 166 141 54 28 EagI C'GGCC_G Cuts at: 0 194 2625 4845 Size: 194 2431 2220 Fragments arranged by size: 2431 2220 194 EarI CTCTTCn'nnn_ Cuts at: 0 439 802 1600 3322 4195 4557 4813 4845 Size: 439 363 798 1722 873 362 256 32 Fragments arranged by size: 1722 873 798 439 363 362 256 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 161 1059 1626 3325 3885 4003 4771 4845 Size: 161 898 567 1699 560 118 768 74 Fragments arranged by size: 1699 898 768 567 560 161 118 74 EcoICRI GAG'CTC Cuts at: 0 2120 4101 4376 4845 Size: 2120 1981 275 469 Fragments arranged by size: 2120 1981 469 275 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 205 631 1642 1912 2107 3418 3466 Size: 178 27 426 1011 270 195 1311 48 Cuts at: 3466 3646 4845 Size: 180 1199 Fragments arranged by size: 1311 1199 1011 426 270 195 180 178 48 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 1537 1621 2644 4845 Size: 1537 84 1023 2201 Fragments arranged by size: 2201 1537 1023 84 EcoO109I rG'GnC_Cy Cuts at: 0 444 1368 3020 3312 3827 4259 4286 4439 Size: 444 924 1652 292 515 432 27 153 Cuts at: 4439 4586 4845 Size: 147 259 Fragments arranged by size: 1652 924 515 444 432 292 259 153 147 27 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 450 666 965 4845 Size: 450 216 299 3880 Fragments arranged by size: 3880 450 299 216 HaeII r_GCGC'y Cuts at: 0 13 64 1695 2451 2991 3837 4242 4612 Size: 13 51 1631 756 540 846 405 370 Cuts at: 4612 4661 4845 Size: 49 184 Fragments arranged by size: 1631 846 756 540 405 370 184 51 49 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 587 619 1946 1978 4684 4716 4845 Size: 587 32 1327 32 2706 32 129 Fragments arranged by size: 2706 1327 587 129 32 32 32 HincII GTy'rAC Cuts at: 0 1233 1269 2175 3222 4845 Size: 1233 36 906 1047 1623 Fragments arranged by size: 1623 1233 1047 906 36 KasI G'GCGC_C Cuts at: 0 2987 4608 4845 Size: 2987 1621 237 Fragments arranged by size: 2987 1621 237 KpnI G_GTAC'C Cuts at: 0 3240 4845 Size: 3240 1605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1343 1779 2078 2516 2851 4118 4845 Size: 1343 436 299 438 335 1267 727 Fragments arranged by size: 1343 1267 727 438 436 335 299 MscI TGG'CCA Cuts at: 0 403 1789 2002 4845 Size: 403 1386 213 2843 Fragments arranged by size: 2843 1386 403 213 MslI CAynn'nnrTG Cuts at: 0 169 397 853 976 1321 2476 2677 3175 Size: 169 228 456 123 345 1155 201 498 Cuts at: 3175 3253 3647 3708 4151 4274 4540 4636 4845 Size: 78 394 61 443 123 266 96 209 Fragments arranged by size: 1155 498 456 443 394 345 266 228 209 201 169 123 123 96 78 61 MspA1I CmG'CkG Cuts at: 0 33 88 179 984 1752 2745 2774 4283 Size: 33 55 91 805 768 993 29 1509 Cuts at: 4283 4667 4766 4845 Size: 384 99 79 Fragments arranged by size: 1509 993 805 768 384 99 91 79 55 33 29 NaeI GCC'GGC Cuts at: 0 144 4845 Size: 144 4701 NarI GG'CG_CC Cuts at: 0 2988 4609 4845 Size: 2988 1621 236 Fragments arranged by size: 2988 1621 236 NcoI C'CATG_G Cuts at: 0 842 3170 4845 Size: 842 2328 1675 Fragments arranged by size: 2328 1675 842 NgoMIV G'CCGG_C Cuts at: 0 142 4845 Size: 142 4703 NotI GC'GGCC_GC Cuts at: 0 194 4845 Size: 194 4651 NspI r_CATG'y Cuts at: 0 495 962 2499 3258 3797 4358 4545 4845 Size: 495 467 1537 759 539 561 187 300 Fragments arranged by size: 1537 759 561 539 495 467 300 187 PasI CC'CwG_GG Cuts at: 0 3206 4845 Size: 3206 1639 PciI A'CATG_T Cuts at: 0 491 4541 4845 Size: 491 4050 304 Fragments arranged by size: 4050 491 304 PflMI CCAn_nnn'nTGG Cuts at: 0 3609 4227 4667 4845 Size: 3609 618 440 178 Fragments arranged by size: 3609 618 440 178 PfoI T'CCnGG_A Cuts at: 0 421 1619 2879 4845 Size: 421 1198 1260 1966 Fragments arranged by size: 1966 1260 1198 421 PmlI CAC'GTG Cuts at: 0 2133 4296 4845 Size: 2133 2163 549 Fragments arranged by size: 2163 2133 549 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2104 2136 4690 4722 4845 Size: 2104 32 2554 32 123 Fragments arranged by size: 2554 2104 123 32 32 PpuMI rG'GwC_Cy Cuts at: 0 444 1368 3020 3312 4286 4845 Size: 444 924 1652 292 974 559 Fragments arranged by size: 1652 974 924 559 444 292 PshAI GACnn'nnGTC Cuts at: 0 1228 2077 4845 Size: 1228 849 2768 Fragments arranged by size: 2768 1228 849 PspOMI G'GGCC_C Cuts at: 0 4162 4258 4845 Size: 4162 96 587 Fragments arranged by size: 4162 587 96 PstI C_TGCA'G Cuts at: 0 989 1103 1538 2824 3743 4630 4845 Size: 989 114 435 1286 919 887 215 Fragments arranged by size: 1286 989 919 887 435 215 114 PvuII CAG'CTG Cuts at: 0 984 1752 2745 4766 4845 Size: 984 768 993 2021 79 Fragments arranged by size: 2021 993 984 768 79 RsrII CG'GwC_CG Cuts at: 0 19 1311 4845 Size: 19 1292 3534 Fragments arranged by size: 3534 1292 19 SacI G_AGCT'C Cuts at: 0 2122 4103 4378 4845 Size: 2122 1981 275 467 Fragments arranged by size: 2122 1981 467 275 SacII CC_GC'GG Cuts at: 0 89 4845 Size: 89 4756 SalI G'TCGA_C Cuts at: 0 1267 4845 Size: 1267 3578 SanDI GG'GwC_CC Cuts at: 0 3020 4286 4845 Size: 3020 1266 559 Fragments arranged by size: 3020 1266 559 SapI GCTCTTCn'nnn_ Cuts at: 0 439 4845 Size: 439 4406 SbfI CC_TGCA'GG Cuts at: 0 1538 4845 Size: 1538 3307 ScaI AGT'ACT Cuts at: 0 4684 4845 Size: 4684 161 SfcI C'TryA_G Cuts at: 0 985 1099 1534 2820 3099 3739 4626 4653 Size: 985 114 435 1286 279 640 887 27 Cuts at: 4653 4845 Size: 192 Fragments arranged by size: 1286 985 887 640 435 279 192 114 27 SfoI GGC'GCC Cuts at: 0 2989 4610 4845 Size: 2989 1621 235 Fragments arranged by size: 2989 1621 235 SgrAI Cr'CCGG_yG Cuts at: 0 142 4845 Size: 142 4703 SmaI CCC'GGG Cuts at: 0 3825 4319 4845 Size: 3825 494 526 Fragments arranged by size: 3825 526 494 SmlI C'TyrA_G Cuts at: 0 1978 4845 Size: 1978 2867 SphI G_CATG'C Cuts at: 0 4358 4845 Size: 4358 487 StuI AGG'CCT Cuts at: 0 1933 2029 4845 Size: 1933 96 2816 Fragments arranged by size: 2816 1933 96 StyI C'CwwG_G Cuts at: 0 218 470 842 1790 2312 3008 3051 3170 Size: 218 252 372 948 522 696 43 119 Cuts at: 3170 3486 3689 4845 Size: 316 203 1156 Fragments arranged by size: 1156 948 696 522 372 316 252 218 203 119 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 339 855 1263 1312 2347 2476 2536 2967 Size: 339 516 408 49 1035 129 60 431 Cuts at: 2967 3372 3659 4189 4743 4845 Size: 405 287 530 554 102 Fragments arranged by size: 1035 554 530 516 431 408 405 339 287 129 102 60 49 TatI w'GTAC_w Cuts at: 0 230 365 494 749 1418 2558 3242 4529 Size: 230 135 129 255 669 1140 684 1287 Cuts at: 4529 4682 4845 Size: 153 163 Fragments arranged by size: 1287 1140 684 669 255 230 163 153 135 129 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1957 1989 4845 Size: 1957 32 2856 Fragments arranged by size: 2856 1957 32 XhoI C'TCGA_G Cuts at: 0 1978 4845 Size: 1978 2867 XmaI C'CCGG_G Cuts at: 0 3823 4317 4845 Size: 3823 494 528 Fragments arranged by size: 3823 528 494 ZraI GAC'GTC Cuts at: 0 1737 2079 4845 Size: 1737 342 2766 Fragments arranged by size: 2766 1737 342 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI XhoI XmaI ZraI Enzymes that do not cut: AclI AflII AgeI AhdI ApoI AscI AseI AsiSI AvrII BaeI BfrBI BglII BmtI BpuEI BsmI BspEI BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI NdeI NheI NruI NsiI PacI PmeI PsiI PspXI PsrI PvuI SexAI SfiI SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI XmnI