Restriction map for
pFN21AE2524
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BbrPI BbsI BcgI BclI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaI BseRI BsiEI
BsiWI BsmAI BsmBI BspDI BspHI
BspLU11I BspMI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MscI MunI NdeI
NheI NotI NruI NsiI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 1097 TCCGGA T^CCGGA
AhdI 124 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 970 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 124 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 481 GGATCC G^GATCC
BbvCI 520 GCTGAGG CCTCAGC(-5/-2)
BciVI 1104 GTATCC GTATCC(6/5)
BglI 42 GCCGCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 57 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 57 GCTGAGC GC^T[ACGT]AGC
BsaWI 1097 TCCGGA [AT]^CCGG[AT]
BseAI 1097 TCCGGA T^CCGGA
BsgI 15 CTGCAC GTGCAG(16/14)
BsmFI 1260 GGGAC GGGAC(10/14)
BsmI 1153 GCATTC GAATGC(1/-1)
Bsp1407I 1275 TGTACA T^GTACA
BspEI 1097 TCCGGA T^CCGGA
BsrDI 872 CATTGC GCAATG(2/0)
BsrFI 632 GCCGGC [GA]^CCGG[CT]
BsrGI 1275 TGTACA T^GTACA
BstBI 991 TTCGAA TT^CGAA
BstNI 305 CCTGG CC^[AT]GG
BstYI 481 GGATCC [GA]^GATC[CT]
CelII 57 GCTGAGC GC^T[ACGT]AGC
Cfr10I 632 GCCGGC [GA]^CCGG[CT]
DsaI 527 CCATGG C^C[GA][CT]GG
EaeI 147 CGGCCA [CT]^GGCC[GA]
Eam1105I 124 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1353 GAAGAG CTCTTC(1/4)
EcoNI 315 CCTCCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 957 GAATTC G^AATTC
EcoRII 305 CCTGG ^CC[AT]GG
HindIII 1360 AAGCTT A^AGCTT
HphI 409 GGTGA GGTGA(8/7)
Ksp632I 1353 GAAGAG CTCTTC(1/4)
MflI 481 GGATCC [GA]^GATC[CT]
MroI 1097 TCCGGA T^CCGGA
MvaI 305 CCTGG CC^[AT]GG
NaeI 632 GCCGGC GCC^GGC
NcoI 527 CCATGG C^CATGG
NgoMIV 632 GCCGGC G^CCGGC
NspI 365 ACATGC [GA]CATG^[CT]
NspV 991 TTCGAA TT^CGAA
PaeR7I 127 CTCGAG C^TCGAG
PleI 130 GAGTC GAGTC(4/5)
PpuMI 463 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1262 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 674 CTGCAG CTGCA^G
PvuII 139 CAGCTG CAG^CTG
ScaI 1180 AGTACT AGT^ACT
SfcI 674 CTGCAG C^T[GA][CT]AG
SfuI 991 TTCGAA TT^CGAA
SmlI 127 CTCGAG C^T[CT][GA]AG
SspBI 1275 TGTACA T^GTACA
Tsp45I 570 GTCAC ^GT[GC]AC
XhoI 127 CTCGAG C^TCGAG
XhoII 481 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 482 GATCC GGATC(4/5)
481 GGATC
AspHI 378 GTGCTC G[AT]GC[AT]^C
427 GTGCTC
AvaII 464 GGTCC G^G[AT]CC
1268 GGTCC
BanI 87 GGCGCC G^G[CT][GA]CC
301 GGCGCC
BanII 34 GAGCCC G[GA]GC[CT]^C
250 GAGCCC
BbeI 87 GGCGCC GGCGC^C
301 GGCGCC
BsiHKAI 378 GTGCTC G[AT]GC[AT]^C
427 GTGCTC
BsrBI 43 CCGCTC CCGCTC(-3/-3)
160 CCGCTC
BstXI 134 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
527 CCATGGTTCTGG
DpnII 482 GATC ^GATC
869 GATC
HaeII 87 GGCGCC [GA]GCGC^[CT]
301 GGCGCC
HgaI 112 GACGC GACGC(5/10)
258 GACGC
KasI 87 GGCGCC G^GCGCC
301 GGCGCC
MboI 482 GATC ^GATC
869 GATC
MslI 334 CACATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1036 CACTTCTGTG
NarI 87 GGCGCC GG^CGCC
301 GGCGCC
NdeII 482 GATC ^GATC
869 GATC
Sau3AI 482 GATC ^GATC
869 GATC
SfoI 87 GGCGCC GGC^GCC
301 GGCGCC
TspRI 339 CAGTG CA[GC]TG[ACGT][ACGT]^
376 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AvaI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstF5I BstUI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeIII MboII MnlI MseI MspA1I
MspI MvnI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp509I TthHB8I