(Circular) (Six-base) MAPSORT of: pFN21AE2524.seq Check: 1248 from: 1 to: 6076 pFN21AE2524 6076 bp With 182 enzymes: SgfI * June 11, 2014 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3886 278 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3419 1937 Size: 1482 4594 Acc65I G'GTAC_C Cuts at: 3397 3397 Size: 6076 AclI AA'CG_TT Cuts at: 1563 4203 4576 1563 Size: 2640 373 3063 Fragments arranged by size: 3063 2640 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2673 3210 4139 5523 5791 1632 Size: 1041 537 929 1384 268 1917 Fragments arranged by size: 1917 1384 1041 929 537 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5879 AflIII A'CryG_T Cuts at: 1203 4975 5952 1203 Size: 3772 977 1327 Fragments arranged by size: 3772 1327 977 AgeI A'CCGG_T Cuts at: 4907 4907 Size: 6076 AhdI GACnn_n'nnGTC Cuts at: 2134 4805 2134 Size: 2671 3405 AleI CACnn'nnGTG Cuts at: 1166 2343 3045 1166 Size: 1177 702 4197 Fragments arranged by size: 4197 1177 702 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6076 AlwNI CAG_nnn'CTG Cuts at: 2980 3819 5391 2980 Size: 839 1572 3665 Fragments arranged by size: 3665 1572 839 ApaLI G'TGCA_C Cuts at: 4133 5289 4133 Size: 1156 4920 ApoI r'AATT_y Cuts at: 1493 2015 2513 2795 2962 3305 3385 3611 3763 Size: 522 498 282 167 343 80 226 152 Cuts at: 3763 5872 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 522 498 343 282 226 167 152 80 AseI AT'TA_AT Cuts at: 160 1032 4630 160 Size: 872 3598 1606 Fragments arranged by size: 3598 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2132 2632 3401 1870 Size: 73 108 81 500 769 4545 Fragments arranged by size: 4545 769 500 108 81 73 BamHI G'GATC_C Cuts at: 2486 3406 3773 2486 Size: 920 367 4789 Fragments arranged by size: 4789 920 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 2306 3397 4852 618 Size: 325 217 627 305 214 1091 1455 1842 Fragments arranged by size: 1842 1455 1091 627 325 305 217 214 BanII G_rGCy'C Cuts at: 1391 2043 2259 3395 1391 Size: 652 216 1136 4072 Fragments arranged by size: 4072 1136 652 216 BbeI G_GCGC'C Cuts at: 2096 2310 2096 Size: 214 5862 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5105 BbvCI CC'TCA_GC Cuts at: 2526 2526 Size: 6076 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4267 4301 1903 Size: 34 2330 34 3678 Fragments arranged by size: 3678 2330 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3120 3968 5178 3120 Size: 848 1210 4018 Fragments arranged by size: 4018 1210 848 BclI T'GATC_A Cuts at: 1548 1548 Size: 6076 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2053 4687 136 Size: 107 122 71 631 605 381 2634 1525 Fragments arranged by size: 2634 1525 631 605 381 122 107 71 BglII A'GATC_T Cuts at: 6071 6071 Size: 6076 BlpI GC'TnA_GC Cuts at: 2063 3493 2063 Size: 1430 4646 Bme1580I G_kGCm'C Cuts at: 1790 4137 5293 1790 Size: 2347 1156 2573 Fragments arranged by size: 2573 2347 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6076 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3807 4765 442 Size: 416 53 83 399 24 2390 958 1753 Fragments arranged by size: 2390 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6076 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2561 2639 2883 4736 1239 Size: 1322 78 244 1853 2579 Fragments arranged by size: 2579 1853 1322 244 78 Bpu10I CC'TnA_GC Cuts at: 2363 2526 2363 Size: 163 5913 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4201 5066 5364 4201 Size: 865 298 4913 Fragments arranged by size: 4913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4739 882 Size: 617 3240 2219 Fragments arranged by size: 3240 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3867 493 Size: 711 2663 2702 Fragments arranged by size: 2702 2663 711 BsaBI GATnn'nnATC Cuts at: 6070 6070 Size: 6076 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2093 2118 2307 3883 Size: 53 83 186 936 560 25 189 1576 Cuts at: 3883 4265 4900 275 Size: 382 635 1451 Fragments arranged by size: 1576 1451 936 635 560 382 189 186 83 53 25 BsaWI w'CCGG_w Cuts at: 3102 4509 4907 5181 5328 5739 3102 Size: 1407 398 274 147 411 3439 Fragments arranged by size: 3439 1407 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2881 2911 3114 3144 1178 Size: 30 165 30 1478 30 203 30 4110 Fragments arranged by size: 4110 1478 203 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6076 BseYI C'CCAG_C Cuts at: 2146 2214 5279 5775 2146 Size: 68 3065 496 2447 Fragments arranged by size: 3065 2447 496 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2005 1163 Size: 573 269 5234 Fragments arranged by size: 5234 573 269 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3526 4287 5315 1540 Size: 275 186 1525 761 1028 2301 Fragments arranged by size: 2301 1525 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2387 2436 4137 4222 5293 2387 Size: 49 1701 85 1071 3170 Fragments arranged by size: 3170 1701 1071 85 49 BsmI GAATG_Cn' Cuts at: 3158 3587 3680 3158 Size: 429 93 5554 Fragments arranged by size: 5554 429 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2259 2387 2436 3395 4137 4222 Size: 399 253 216 128 49 959 742 85 Cuts at: 4222 5293 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 959 742 399 253 216 128 85 49 BspEI T'CCGG_A Cuts at: 3102 5739 3102 Size: 2637 3439 BspHI T'CATG_A Cuts at: 3965 5695 3965 Size: 1730 4346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3431 844 Size: 2587 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2050 2167 3963 1394 Size: 429 227 117 1796 3507 Fragments arranged by size: 3507 1796 429 227 117 BsrDI GCAATG_nn' Cuts at: 65 1248 2876 4571 4745 65 Size: 1183 1628 1695 174 1396 Fragments arranged by size: 1695 1628 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2637 4720 4907 1700 Size: 110 827 2083 187 2869 Fragments arranged by size: 2869 2083 827 187 110 BsrGI T'GTAC_A Cuts at: 96 3280 96 Size: 3184 2892 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6076 BssSI C'ACGA_G Cuts at: 4136 5148 4136 Size: 1012 5064 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6076 BstBI TT'CG_AA Cuts at: 2997 4880 2997 Size: 1883 4193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6076 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2539 2146 Size: 393 5683 BstYI r'GATC_y Cuts at: 1972 2486 3406 3773 4158 4175 5627 5713 5725 Size: 514 920 367 385 17 1452 86 12 Cuts at: 5725 6071 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 920 514 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2532 513 Size: 552 1467 4057 Fragments arranged by size: 4057 1467 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5337 Fragments arranged by size: 5337 683 56 BtsI GCAGTG_nn' Cuts at: 3592 4386 4406 3592 Size: 794 20 5262 Fragments arranged by size: 5262 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6076 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6041 DraI TTT'AAA Cuts at: 3380 3380 Size: 6076 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5083 809 Size: 507 3767 1802 Fragments arranged by size: 3767 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2152 3523 4412 Size: 54 1425 50 275 18 322 1371 889 Cuts at: 4412 6041 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1371 889 322 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3523 1537 Size: 275 1711 4090 Fragments arranged by size: 4090 1711 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3353 4006 1353 Size: 2000 653 3423 Fragments arranged by size: 3423 2000 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2051 2172 4663 5037 5183 387 Size: 1064 138 462 121 2491 374 146 1280 Fragments arranged by size: 2491 1280 1064 462 374 146 138 121 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2561 2639 2673 2883 3210 4139 4736 Size: 393 929 78 34 210 327 929 597 Cuts at: 4736 5523 5791 1239 Size: 787 268 1524 Fragments arranged by size: 1524 929 929 787 597 393 327 268 210 78 34 EcoNI CCTnn'n_nnAGG Cuts at: 2324 2324 Size: 6076 EcoO109I rG'GnC_Cy Cuts at: 2274 2317 2469 2274 Size: 43 152 5881 Fragments arranged by size: 5881 152 43 EcoRI G'AATT_C Cuts at: 2962 3385 3763 2962 Size: 423 378 5275 Fragments arranged by size: 5275 423 378 FspI TGC'GCA Cuts at: 4582 4582 Size: 6076 HaeII r_GCGC'y Cuts at: 1678 2096 2310 5223 1678 Size: 418 214 2913 2531 Fragments arranged by size: 2913 2531 418 214 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 3084 3116 4717 Size: 32 135 32 548 32 932 32 1601 Cuts at: 4717 4749 4791 4823 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1601 932 548 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3420 3666 669 Size: 988 281 1482 246 3079 Fragments arranged by size: 3079 1482 988 281 246 HindIII A'AGCT_T Cuts at: 748 3365 748 Size: 2617 3459 HpaI GTT'AAC Cuts at: 3666 3666 Size: 6076 KasI G'GCGC_C Cuts at: 2092 2306 2092 Size: 214 5862 KpnI G_GTAC'C Cuts at: 3401 3401 Size: 6076 MfeI C'AATT_G Cuts at: 3675 3675 Size: 6076 MluI A'CGCG_T Cuts at: 5952 5952 Size: 6076 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5190 5374 1305 Size: 3885 184 2007 Fragments arranged by size: 3885 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4254 Fragments arranged by size: 4254 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 3045 4034 4393 4552 518 Size: 648 1177 702 989 359 159 2042 Fragments arranged by size: 2042 1177 989 702 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2191 2197 2203 2209 3492 4169 5317 Size: 540 45 6 6 6 1283 677 1148 Cuts at: 5317 5562 1606 Size: 245 2120 Fragments arranged by size: 2120 1283 1148 677 540 245 45 6 6 6 NaeI GCC'GGC Cuts at: 2639 2639 Size: 6076 NarI GG'CG_CC Cuts at: 2093 2307 2093 Size: 214 5862 NcoI C'CATG_G Cuts at: 513 1065 2532 513 Size: 552 1467 4057 Fragments arranged by size: 4057 1467 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6076 NgoMIV G'CCGG_C Cuts at: 2637 2637 Size: 6076 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6076 NotI GC'GGCC_GC Cuts at: 3523 3523 Size: 6076 NspI r_CATG'y Cuts at: 1132 1229 2374 3434 4979 1132 Size: 97 1145 1060 1545 2229 Fragments arranged by size: 2229 1545 1145 1060 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6076 PciI A'CATG_T Cuts at: 4975 4975 Size: 6076 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6076 PmeI GTTT'AAAC Cuts at: 3380 3380 Size: 6076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2318 2350 4145 4177 5658 5690 2318 Size: 32 1795 32 1481 32 2704 Fragments arranged by size: 2704 1795 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2469 2469 Size: 6076 PshAI GACnn'nnGTC Cuts at: 1613 3271 1613 Size: 1658 4418 PsiI TTA'TAA Cuts at: 3206 3646 3206 Size: 440 5636 PspXI vC'TCGA_Gb Cuts at: 2132 2132 Size: 6076 PstI C_TGCA'G Cuts at: 830 2683 3428 830 Size: 1853 745 3478 Fragments arranged by size: 3478 1853 745 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6076 PvuII CAG'CTG Cuts at: 2146 2146 Size: 6076 SalI G'TCGA_C Cuts at: 1936 3418 1936 Size: 1482 4594 SbfI CC_TGCA'GG Cuts at: 3428 3428 Size: 6076 ScaI AGT'ACT Cuts at: 3187 4324 6036 3187 Size: 1137 1712 3227 Fragments arranged by size: 3227 1712 1137 SfcI C'TryA_G Cuts at: 826 1045 2679 3424 4559 5240 5431 826 Size: 219 1634 745 1135 681 191 1471 Fragments arranged by size: 1634 1471 1135 745 681 219 191 SfoI GGC'GCC Cuts at: 2094 2308 2094 Size: 214 5862 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6076 SmaI CCC'GGG Cuts at: 1872 3403 1872 Size: 1531 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 4180 5081 5343 820 Size: 197 926 189 2048 901 262 1553 Fragments arranged by size: 2048 1553 926 901 262 197 189 SnaBI TAC'GTA Cuts at: 493 3867 493 Size: 3374 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6076 SphI G_CATG'C Cuts at: 1132 3434 1132 Size: 2302 3774 SspI AAT'ATT Cuts at: 5 52 4000 5 Size: 47 3948 2081 Fragments arranged by size: 3948 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6076 StyI C'CwwG_G Cuts at: 513 1065 2410 2532 2608 2853 3515 513 Size: 552 1345 122 76 245 662 3074 Fragments arranged by size: 3074 1345 662 552 245 122 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4103 4120 4273 4458 4903 325 Size: 1255 2523 17 153 185 445 1498 Fragments arranged by size: 2523 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3185 3280 4322 Size: 274 80 33 51 1443 1208 95 1042 Cuts at: 4322 6034 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1208 1042 274 138 95 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6036 6068 6036 Size: 32 6044 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6076 XbaI T'CTAG_A Cuts at: 3412 3412 Size: 6076 XhoI C'TCGA_G Cuts at: 1943 2132 1943 Size: 189 5887 XmaI C'CCGG_G Cuts at: 1870 3401 1870 Size: 1531 4545 XmnI GAAnn'nnTTC Cuts at: 4205 4205 Size: 6076 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3884 276 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI