(Linear) (Six-base) MAPSORT of: KIEE2524.seq Check: 8717 from: 1 to: 1371 KIEE2524.orf With 182 enzymes: SgfI * June 11, 2014 14:42 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 668 1205 1371 Size: 668 537 166 Fragments arranged by size: 668 537 166 AhdI GACnn_n'nnGTC Cuts at: 0 129 1371 Size: 129 1242 AleI CACnn'nnGTG Cuts at: 0 338 1040 1371 Size: 338 702 331 Fragments arranged by size: 702 338 331 AlwNI CAG_nnn'CTG Cuts at: 0 975 1371 Size: 975 396 ApoI r'AATT_y Cuts at: 0 10 508 790 957 1300 1371 Size: 10 498 282 167 343 71 Fragments arranged by size: 498 343 282 167 71 10 AvaI C'yCGr_G Cuts at: 0 46 127 627 1371 Size: 46 81 500 744 Fragments arranged by size: 744 500 81 46 BamHI G'GATC_C Cuts at: 0 481 1371 Size: 481 890 BanI G'GyrC_C Cuts at: 0 87 301 1371 Size: 87 214 1070 Fragments arranged by size: 1070 214 87 BanII G_rGCy'C Cuts at: 0 38 254 1371 Size: 38 216 1117 Fragments arranged by size: 1117 216 38 BbeI G_GCGC'C Cuts at: 0 91 305 1371 Size: 91 214 1066 Fragments arranged by size: 1066 214 91 BbvCI CC'TCA_GC Cuts at: 0 521 1371 Size: 521 850 BciVI GTATCCnnnnn_n' Cuts at: 0 1115 1371 Size: 1115 256 BglI GCCn_nnn'nGGC Cuts at: 0 48 1371 Size: 48 1323 BlpI GC'TnA_GC Cuts at: 0 58 1371 Size: 58 1313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 634 878 1371 Size: 556 78 244 493 Fragments arranged by size: 556 493 244 78 Bpu10I CC'TnA_GC Cuts at: 0 358 521 1371 Size: 358 163 850 Fragments arranged by size: 850 358 163 BsaHI Gr'CG_yC Cuts at: 0 88 113 302 1371 Size: 88 25 189 1069 Fragments arranged by size: 1069 189 88 25 BsaWI w'CCGG_w Cuts at: 0 1097 1371 Size: 1097 274 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 876 906 1109 1139 1371 Size: 876 30 203 30 232 Fragments arranged by size: 876 232 203 30 30 BseYI C'CCAG_C Cuts at: 0 141 209 1371 Size: 141 68 1162 Fragments arranged by size: 1162 141 68 BsiHKAI G_wGCw'C Cuts at: 0 382 431 1371 Size: 382 49 940 Fragments arranged by size: 940 382 49 BsmI GAATG_Cn' Cuts at: 0 1153 1371 Size: 1153 218 Bsp1286I G_dGCh'C Cuts at: 0 38 254 382 431 1371 Size: 38 216 128 49 940 Fragments arranged by size: 940 216 128 49 38 BspEI T'CCGG_A Cuts at: 0 1097 1371 Size: 1097 274 BsrBI CCG'CTC Cuts at: 0 45 162 1371 Size: 45 117 1209 Fragments arranged by size: 1209 117 45 BsrDI GCAATG_nn' Cuts at: 0 871 1371 Size: 871 500 BsrFI r'CCGG_y Cuts at: 0 632 1371 Size: 632 739 BsrGI T'GTAC_A Cuts at: 0 1275 1371 Size: 1275 96 BstBI TT'CG_AA Cuts at: 0 992 1371 Size: 992 379 BstXI CCAn_nnnn'nTGG Cuts at: 0 141 534 1371 Size: 141 393 837 Fragments arranged by size: 837 393 141 BstYI r'GATC_y Cuts at: 0 481 1371 Size: 481 890 BtgI C'CryG_G Cuts at: 0 527 1371 Size: 527 844 EaeI y'GGCC_r Cuts at: 0 147 1371 Size: 147 1224 EarI CTCTTCn'nnn_ Cuts at: 0 1348 1371 Size: 1348 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 46 167 1371 Size: 46 121 1204 Fragments arranged by size: 1204 121 46 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 634 668 878 1205 1371 Size: 556 78 34 210 327 166 Fragments arranged by size: 556 327 210 166 78 34 EcoNI CCTnn'n_nnAGG Cuts at: 0 319 1371 Size: 319 1052 EcoO109I rG'GnC_Cy Cuts at: 0 269 312 464 1371 Size: 269 43 152 907 Fragments arranged by size: 907 269 152 43 EcoRI G'AATT_C Cuts at: 0 957 1371 Size: 957 414 HaeII r_GCGC'y Cuts at: 0 91 305 1371 Size: 91 214 1066 Fragments arranged by size: 1066 214 91 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 115 147 1079 1111 1371 Size: 115 32 932 32 260 Fragments arranged by size: 932 260 115 32 32 HindIII A'AGCT_T Cuts at: 0 1360 1371 Size: 1360 11 KasI G'GCGC_C Cuts at: 0 87 301 1371 Size: 87 214 1070 Fragments arranged by size: 1070 214 87 MslI CAynn'nnrTG Cuts at: 0 338 1040 1371 Size: 338 702 331 Fragments arranged by size: 702 338 331 MspA1I CmG'CkG Cuts at: 0 141 186 192 198 204 1371 Size: 141 45 6 6 6 1167 Fragments arranged by size: 1167 141 45 6 6 6 NaeI GCC'GGC Cuts at: 0 634 1371 Size: 634 737 NarI GG'CG_CC Cuts at: 0 88 302 1371 Size: 88 214 1069 Fragments arranged by size: 1069 214 88 NcoI C'CATG_G Cuts at: 0 527 1371 Size: 527 844 NgoMIV G'CCGG_C Cuts at: 0 632 1371 Size: 632 739 NspI r_CATG'y Cuts at: 0 369 1371 Size: 369 1002 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 313 345 1371 Size: 313 32 1026 Fragments arranged by size: 1026 313 32 PpuMI rG'GwC_Cy Cuts at: 0 464 1371 Size: 464 907 PshAI GACnn'nnGTC Cuts at: 0 1266 1371 Size: 1266 105 PsiI TTA'TAA Cuts at: 0 1201 1371 Size: 1201 170 PspXI vC'TCGA_Gb Cuts at: 0 127 1371 Size: 127 1244 PstI C_TGCA'G Cuts at: 0 678 1371 Size: 678 693 PvuII CAG'CTG Cuts at: 0 141 1371 Size: 141 1230 ScaI AGT'ACT Cuts at: 0 1182 1371 Size: 1182 189 SfcI C'TryA_G Cuts at: 0 674 1371 Size: 674 697 SfoI GGC'GCC Cuts at: 0 89 303 1371 Size: 89 214 1068 Fragments arranged by size: 1068 214 89 SmlI C'TyrA_G Cuts at: 0 127 1371 Size: 127 1244 StyI C'CwwG_G Cuts at: 0 405 527 603 848 1371 Size: 405 122 76 245 523 Fragments arranged by size: 523 405 245 122 76 TatI w'GTAC_w Cuts at: 0 1180 1275 1371 Size: 1180 95 96 Fragments arranged by size: 1180 96 95 XhoI C'TCGA_G Cuts at: 0 127 1371 Size: 127 1244 Enzymes that do cut: AcuI AhdI AleI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbvCI BciVI BglI BlpI BpmI Bpu10I BsaHI BsaWI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI BstBI BstXI BstYI BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HindIII KasI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PpiI PpuMI PshAI PsiI PspXI PstI PvuII ScaI SfcI SfoI SmlI StyI TatI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbsI BcgI BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BseRI BsgI BsiEI BsiWI BsmBI BspHI BspMI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MmeI MscI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XmaI XmnI ZraI