################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstYI BstZ17I ClaI CpoI DpnII DraI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI MamI MboI MfeI MflI MluI MseI MslI MunI NdeI NdeII NheI NruI NsiI NspI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI Sau3AI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tsp509I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 627 TCCGGA T^CCGGA AfaI 1314 GTAC GT^AC AgeI 956 ACCGGT A^CCGGT AocI 1250 CCTCAGG CC^T[ACGT]AGG AspI 231 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1534 CACGTG CAC^GTG BbsI 1594 GTCTTC GAAGAC(2/6) BbvCI 378 CCTCAGC CCTCAGC(-5/-2) BfaI 430 CTAG C^TAG BpuAI 1594 GTCTTC GAAGAC(2/6) BsaAI 1534 CACGTG [CT]AC^GT[GA] BsaI 1333 GAGACC GGTCTC(1/5) BseAI 627 TCCGGA T^CCGGA BspEI 627 TCCGGA T^CCGGA BssSI 644 CTCGTG CACGAG(-5/-1) Bsu36I 1250 CCTCAGG CC^T[ACGT]AGG DraIII 1825 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1203 GACGCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1891 GAAGAG CTCTTC(1/4) Eco81I 1250 CCTCAGG CC^T[ACGT]AGG EcoNI 468 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 789 GGCCGGCC GGCCGG^CC Ksp632I 1891 GAAGAG CTCTTC(1/4) KspI 1717 CCGCGG CCGC^GG MaeI 430 CTAG C^TAG MroI 627 TCCGGA T^CCGGA NcoI 1116 CCATGG C^CATGG NotI 40 GCGGCCGC GC^GGCCGC PaeR7I 283 CTCGAG C^TCGAG PflFI 231 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 956 ACCGGT A^CCGGT PmaCI 1534 CACGTG CAC^GTG PmlI 1534 CACGTG CAC^GTG RsaI 1314 GTAC GT^AC SacII 1717 CCGCGG CCGC^GG SbfI 1524 CCTGCAGG CCTGCA^GG SfiI 784 GGCCTGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 1524 CCTGCAGG CCTGCA^GG Tth111I 231 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 283 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 70 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 181 CAGGCCCTG BalI 29 TGGCCA TGG^CCA 614 TGGCCA BseRI 972 CTCCTC GAGGAG(10/8) 1802 GAGGAG BsmAI 1333 GAGAC GTCTC(1/5) 237 GTCTC BsrDI 846 CATTGC GCAATG(2/0) 242 GCAATG EcoT14I 1116 CCATGG C^C[AT][AT]GG 1703 CCAAGG HinfI 82 GAGTC G^A[ACGT]TC 1210 GAGTC MluNI 29 TGGCCA TGG^CCA 614 TGGCCA MscI 29 TGGCCA TGG^CCA 614 TGGCCA PleI 82 GAGTC GAGTC(4/5) 1210 GAGTC PstI 364 CTGCAG CTGCA^G 1525 CTGCAG PvuII 391 CAGCTG CAG^CTG 1746 CAGCTG SfcI 364 CTGCAG C^T[GA][CT]AG 1525 CTGCAG SmlI 283 CTCGAG C^T[CT][GA]AG 1651 CTCAAG StuI 111 AGGCCT AGG^CCT 662 AGGCCT StyI 1116 CCATGG C^C[AT][AT]GG 1703 CCAAGG TaqI 284 TCGA T^CGA 419 TCGA TthHB8I 284 TCGA T^CGA 419 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BsaWI BsgI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoO65I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeII MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV PflMI PpuMI Sau96I ScrFI SfaNI SfoI SmaI TseI Tsp45I TspRI Van91I XcmI XmaCI XmaI