(Linear) (Six-base) MAPSORT of: KIEE1174.seq Check: 6066 from: 1 to: 1905 KIEE1174.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 465 1057 1218 1905 Size: 465 592 161 687 Fragments arranged by size: 687 592 465 161 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1402 1905 Size: 1402 503 AgeI A'CCGG_T Cuts at: 0 956 1905 Size: 956 949 AlwNI CAG_nnn'CTG Cuts at: 0 75 186 1905 Size: 75 111 1719 Fragments arranged by size: 1719 111 75 ApaI G_GGCC'C Cuts at: 0 194 267 355 912 1157 1448 1815 1905 Size: 194 73 88 557 245 291 367 90 Fragments arranged by size: 557 367 291 245 194 90 88 73 AvaI C'yCGr_G Cuts at: 0 283 814 977 983 1085 1150 1273 1846 Size: 283 531 163 6 102 65 123 573 Cuts at: 1846 1905 Size: 59 Fragments arranged by size: 573 531 283 163 123 102 65 59 6 BanI G'GyrC_C Cuts at: 0 334 477 496 559 902 1793 1856 1905 Size: 334 143 19 63 343 891 63 49 Fragments arranged by size: 891 343 334 143 63 63 49 19 BanII G_rGCy'C Cuts at: 0 194 267 355 912 984 1157 1448 1726 Size: 194 73 88 557 72 173 291 278 Cuts at: 1726 1815 1905 Size: 89 90 Fragments arranged by size: 557 291 278 194 173 90 89 88 73 72 BbeI G_GCGC'C Cuts at: 0 500 563 1797 1905 Size: 500 63 1234 108 Fragments arranged by size: 1234 500 108 63 BbsI GAAGACnn'nnnn_ Cuts at: 0 1587 1905 Size: 1587 318 BbvCI CC'TCA_GC Cuts at: 0 379 1905 Size: 379 1526 BglI GCCn_nnn'nGGC Cuts at: 0 350 622 791 1528 1905 Size: 350 272 169 737 377 Fragments arranged by size: 737 377 350 272 169 BlpI GC'TnA_GC Cuts at: 0 88 685 1657 1905 Size: 88 597 972 248 Fragments arranged by size: 972 597 248 88 Bme1580I G_kGCm'C Cuts at: 0 105 194 267 337 355 482 815 912 Size: 105 89 73 70 18 127 333 97 Cuts at: 912 1157 1448 1485 1815 1905 Size: 245 291 37 330 90 Fragments arranged by size: 333 330 291 245 127 105 97 90 89 73 70 37 18 BmgBI CAC'GTC Cuts at: 0 1665 1905 Size: 1665 240 BmrI ACTGGGnnnn_n' Cuts at: 0 948 1285 1905 Size: 948 337 620 Fragments arranged by size: 948 620 337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 1228 1347 1905 Size: 460 768 119 558 Fragments arranged by size: 768 558 460 119 Bpu10I CC'TnA_GC Cuts at: 0 379 1905 Size: 379 1526 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1636 1905 Size: 1636 269 BsaI GGTCTCn'nnnn_ Cuts at: 0 1327 1905 Size: 1327 578 BsaAI yAC'GTr Cuts at: 0 1536 1905 Size: 1536 369 BsaHI Gr'CG_yC Cuts at: 0 497 560 1794 1905 Size: 497 63 1234 111 Fragments arranged by size: 1234 497 111 63 BsaWI w'CCGG_w Cuts at: 0 627 956 1346 1905 Size: 627 329 390 559 Fragments arranged by size: 627 559 390 329 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 586 609 616 639 1905 Size: 586 23 7 23 1266 Fragments arranged by size: 1266 586 23 23 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 963 1817 1905 Size: 963 854 88 Fragments arranged by size: 963 854 88 BseYI C'CCAG_C Cuts at: 0 330 393 569 1027 1036 1265 1356 1441 Size: 330 63 176 458 9 229 91 85 Cuts at: 1441 1473 1539 1690 1905 Size: 32 66 151 215 Fragments arranged by size: 458 330 229 215 176 151 91 85 66 63 32 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 496 691 1246 1905 Size: 496 195 555 659 Fragments arranged by size: 659 555 496 195 BsiEI CG_ry'CG Cuts at: 0 44 751 795 957 1439 1738 1905 Size: 44 707 44 162 482 299 167 Fragments arranged by size: 707 482 299 167 162 44 44 Bsp1286I G_dGCh'C Cuts at: 0 105 194 267 337 355 482 815 912 Size: 105 89 73 70 18 127 333 97 Cuts at: 912 984 1157 1448 1485 1726 1815 1905 Size: 72 173 291 37 241 89 90 Fragments arranged by size: 333 291 241 173 127 105 97 90 89 89 73 72 70 37 18 BspEI T'CCGG_A Cuts at: 0 627 1905 Size: 627 1278 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 465 1057 1218 1905 Size: 465 592 161 687 Fragments arranged by size: 687 592 465 161 BsrBI CCG'CTC Cuts at: 0 12 40 1177 1884 1905 Size: 12 28 1137 707 21 Fragments arranged by size: 1137 707 28 21 12 BsrDI GCAATG_nn' Cuts at: 0 249 845 1905 Size: 249 596 1060 Fragments arranged by size: 1060 596 249 BsrFI r'CCGG_y Cuts at: 0 140 487 790 956 1021 1434 1500 1905 Size: 140 347 303 166 65 413 66 405 Fragments arranged by size: 413 405 347 303 166 140 66 65 BssSI C'ACGA_G Cuts at: 0 644 1905 Size: 644 1261 BstEII G'GTnAC_C Cuts at: 0 916 1017 1239 1905 Size: 916 101 222 666 Fragments arranged by size: 916 666 222 101 BstXI CCAn_nnnn'nTGG Cuts at: 0 612 1473 1681 1797 1905 Size: 612 861 208 116 108 Fragments arranged by size: 861 612 208 116 108 Bsu36I CC'TnA_GG Cuts at: 0 1251 1905 Size: 1251 654 BtgI C'CryG_G Cuts at: 0 1116 1556 1562 1717 1905 Size: 1116 440 6 155 188 Fragments arranged by size: 1116 440 188 155 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 387 1038 1149 1905 Size: 387 651 111 756 Fragments arranged by size: 756 651 387 111 BtsI GCAGTG_nn' Cuts at: 0 547 708 815 1137 1181 1905 Size: 547 161 107 322 44 724 Fragments arranged by size: 724 547 322 161 107 44 DraIII CAC_nnn'GTG Cuts at: 0 1830 1905 Size: 1830 75 DrdI GACnn_nn'nnGTC Cuts at: 0 1209 1905 Size: 1209 696 EaeI y'GGCC_r Cuts at: 0 29 41 614 748 788 792 1279 1436 Size: 29 12 573 134 40 4 487 157 Cuts at: 1436 1460 1559 1638 1735 1905 Size: 24 99 79 97 170 Fragments arranged by size: 573 487 170 157 134 99 97 79 40 29 24 12 4 EagI C'GGCC_G Cuts at: 0 41 748 792 1436 1735 1905 Size: 41 707 44 644 299 170 Fragments arranged by size: 707 644 299 170 44 41 EarI CTCTTCn'nnn_ Cuts at: 0 1886 1905 Size: 1886 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 1228 1347 1402 1905 Size: 460 768 119 55 503 Fragments arranged by size: 768 503 460 119 55 EcoNI CCTnn'n_nnAGG Cuts at: 0 472 1905 Size: 472 1433 EcoO109I rG'GnC_Cy Cuts at: 0 183 190 191 263 279 351 352 444 Size: 183 7 1 72 16 72 1 92 Cuts at: 444 909 1001 1154 1270 1445 1547 1687 1905 Size: 465 92 153 116 175 102 140 218 Fragments arranged by size: 465 218 183 175 153 140 116 102 92 92 72 72 16 7 1 1 FseI GG_CCGG'CC Cuts at: 0 794 1905 Size: 794 1111 HaeII r_GCGC'y Cuts at: 0 500 563 867 1028 1403 1797 1880 1905 Size: 500 63 304 161 375 394 83 25 Fragments arranged by size: 500 394 375 304 161 83 63 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 222 254 586 618 1254 1286 1905 Size: 222 32 332 32 636 32 619 Fragments arranged by size: 636 619 332 222 32 32 32 KasI G'GCGC_C Cuts at: 0 496 559 1793 1905 Size: 496 63 1234 112 Fragments arranged by size: 1234 496 112 63 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 629 1905 Size: 629 1276 MscI TGG'CCA Cuts at: 0 31 616 1905 Size: 31 585 1289 Fragments arranged by size: 1289 585 31 MspA1I CmG'CkG Cuts at: 0 54 383 393 888 1441 1719 1748 1905 Size: 54 329 10 495 553 278 29 157 Fragments arranged by size: 553 495 329 278 157 54 29 10 NaeI GCC'GGC Cuts at: 0 489 792 1502 1905 Size: 489 303 710 403 Fragments arranged by size: 710 489 403 303 NarI GG'CG_CC Cuts at: 0 497 560 1794 1905 Size: 497 63 1234 111 Fragments arranged by size: 1234 497 111 63 NcoI C'CATG_G Cuts at: 0 1116 1905 Size: 1116 789 NgoMIV G'CCGG_C Cuts at: 0 487 790 1500 1905 Size: 487 303 710 405 Fragments arranged by size: 710 487 405 303 NotI GC'GGCC_GC Cuts at: 0 41 1905 Size: 41 1864 PasI CC'CwG_GG Cuts at: 0 186 482 912 1905 Size: 186 296 430 993 Fragments arranged by size: 993 430 296 186 PflMI CCAn_nnn'nTGG Cuts at: 0 186 938 1539 1905 Size: 186 752 601 366 Fragments arranged by size: 752 601 366 186 PmlI CAC'GTG Cuts at: 0 1536 1905 Size: 1536 369 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 279 311 808 840 1905 Size: 279 32 497 32 1065 Fragments arranged by size: 1065 497 279 32 32 PpuMI rG'GwC_Cy Cuts at: 0 279 444 1001 1270 1547 1905 Size: 279 165 557 269 277 358 Fragments arranged by size: 557 358 279 277 269 165 PspOMI G'GGCC_C Cuts at: 0 190 263 351 908 1153 1444 1811 1905 Size: 190 73 88 557 245 291 367 94 Fragments arranged by size: 557 367 291 245 190 94 88 73 PstI C_TGCA'G Cuts at: 0 368 1529 1905 Size: 368 1161 376 Fragments arranged by size: 1161 376 368 PvuII CAG'CTG Cuts at: 0 393 1748 1905 Size: 393 1355 157 Fragments arranged by size: 1355 393 157 SacII CC_GC'GG Cuts at: 0 1720 1905 Size: 1720 185 SanDI GG'GwC_CC Cuts at: 0 1270 1547 1905 Size: 1270 277 358 Fragments arranged by size: 1270 358 277 SbfI CC_TGCA'GG Cuts at: 0 1529 1905 Size: 1529 376 SfcI C'TryA_G Cuts at: 0 364 1525 1905 Size: 364 1161 380 Fragments arranged by size: 1161 380 364 SfiI GGCCn_nnn'nGGCC Cuts at: 0 791 1905 Size: 791 1114 SfoI GGC'GCC Cuts at: 0 498 561 1795 1905 Size: 498 63 1234 110 Fragments arranged by size: 1234 498 110 63 SmaI CCC'GGG Cuts at: 0 985 1152 1275 1905 Size: 985 167 123 630 Fragments arranged by size: 985 630 167 123 SmlI C'TyrA_G Cuts at: 0 283 1651 1905 Size: 283 1368 254 Fragments arranged by size: 1368 283 254 SrfI GCCC'GGGC Cuts at: 0 985 1905 Size: 985 920 StuI AGG'CCT Cuts at: 0 113 664 1905 Size: 113 551 1241 Fragments arranged by size: 1241 551 113 StyI C'CwwG_G Cuts at: 0 1116 1703 1905 Size: 1116 587 202 Fragments arranged by size: 1116 587 202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 565 861 904 934 1716 1905 Size: 565 296 43 30 782 189 Fragments arranged by size: 782 565 296 189 43 30 TatI w'GTAC_w Cuts at: 0 1313 1905 Size: 1313 592 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 609 641 1116 1148 1654 1686 1905 Size: 609 32 475 32 506 32 219 Fragments arranged by size: 609 506 475 219 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 234 1905 Size: 234 1671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1254 1470 1831 1905 Size: 1254 216 361 74 Fragments arranged by size: 1254 361 216 74 XhoI C'TCGA_G Cuts at: 0 283 1905 Size: 283 1622 XmaI C'CCGG_G Cuts at: 0 983 1150 1273 1905 Size: 983 167 123 632 Fragments arranged by size: 983 632 167 123 Enzymes that do cut: AarI AcuI AgeI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NotI PasI PflMI PmlI PpiI PpuMI PspOMI PstI PvuII SacII SanDI SbfI SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII BmtI BplI BsaBI BsiHKAI BsiWI BsmI BsmBI BspHI BsrGI BssHII BstAPI BstBI BstYI BstZ17I ClaI CspCI DraI EciI EcoICRI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NdeI NheI NruI NsiI NspI PacI PciI PfoI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XmnI ZraI