################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI MamI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1608 GGTACC G^GTACC AcyI 28 GACGCC G[GA]^CG[CT]C Alw44I 1721 GTGCAC G^TGCAC Aor51HI 123 AGCGCT AGC^GCT ApaLI 1721 GTGCAC G^TGCAC Asp718I 1608 GGTACC G^GTACC AviII 75 TGCGCA TGC^GCA BbsI 745 GTCTTC GAAGAC(2/6) BclI 1190 TGATCA T^GATCA BlpI 861 GCTGAGC GC^T[ACGT]AGC Bpu1102I 861 GCTGAGC GC^T[ACGT]AGC BpuAI 745 GTCTTC GAAGAC(2/6) BsaHI 28 GACGCC G[GA]^CG[CT]C BsaI 1645 GAGACC GGTCTC(1/5) BseRI 490 GAGGAG GAGGAG(10/8) BsiEI 1772 CGGCCG CG[GA][CT]^CG BsmBI 481 GAGACG CGTCTC(1/5) BsmI 1014 GCATTC GAATGC(1/-1) Bsp1407I 1565 TGTACA T^GTACA BspHI 1166 TCATGA T^CATGA BsrBI 1321 GAGCGG CCGCTC(-3/-3) BsrFI 1136 GCCGGT [GA]^CCGG[CT] BsrGI 1565 TGTACA T^GTACA BstBI 60 TTCGAA TT^CGAA BstYI 1199 AGATCC [GA]^GATC[CT] CelII 861 GCTGAGC GC^T[ACGT]AGC Cfr10I 1136 GCCGGT [GA]^CCGG[CT] DraIII 1465 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1772 CGGCCG C^GGCCG EarI 827 GAAGAG CTCTTC(1/4) EclXI 1772 CGGCCG C^GGCCG Eco47III 123 AGCGCT AGC^GCT Eco52I 1772 CGGCCG C^GGCCG FbaI 1190 TGATCA T^GATCA FspI 75 TGCGCA TGC^GCA Hin1I 28 GACGCC G[GA]^CG[CT]C HincII 1744 GTCAAC GT[CT]^[GA]AC HindII 1744 GTCAAC GT[CT]^[GA]AC KpnI 1608 GGTACC GGTAC^C Ksp632I 827 GAAGAG CTCTTC(1/4) KspI 791 CCGCGG CCGC^GG MfeI 382 CAATTG C^AATTG MflI 1199 AGATCC [GA]^GATC[CT] MunI 382 CAATTG C^AATTG NotI 1771 GCGGCCGC GC^GGCCGC NspI 1748 ACATGC [GA]CATG^[CT] NspV 60 TTCGAA TT^CGAA PflMI 870 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1166 TCATGA T^CATGA SacI 679 GAGCTC GAGCT^C SacII 791 CCGCGG CCGC^GG ScaI 848 AGTACT AGT^ACT SfuI 60 TTCGAA TT^CGAA SspBI 1565 TGTACA T^GTACA StuI 701 AGGCCT AGG^CCT Van91I 870 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1199 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 792 CGCG CG^CG 1393 CGCG AlwI 1200 GATCC GGATC(4/5) 1305 GGATC AlwNI 257 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1309 CAGACACTG BglI 929 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1033 GCCGTCATGGC BsaAI 1300 TACGTG [CT]AC^GT[GA] 1382 TACGTG BsgI 1462 CTGCAC GTGCAG(16/14) 970 GTGCAG BsrDI 47 GCAATG GCAATG(2/0) 803 GCAATG BstUI 792 CGCG CG^CG 1393 CGCG DsaI 791 CCGCGG C^C[GA][CT]GG 1469 CCGTGG EcoT14I 174 CCTTGG C^C[AT][AT]GG 1482 CCTTGG MseI 448 TTAA T^TAA 1496 TTAA MvnI 792 CGCG CG^CG 1393 CGCG PaeR7I 1175 CTCGAG C^TCGAG 1411 CTCGAG PvuII 257 CAGCTG CAG^CTG 526 CAGCTG SbfI 1026 CCTGCAGG CCTGCA^GG 1401 CCTGCAGG SmaI 1019 CCCGGG CCC^GGG 1592 CCCGGG Sse8387I 1026 CCTGCAGG CCTGCA^GG 1401 CCTGCAGG StyI 174 CCTTGG C^C[AT][AT]GG 1482 CCTTGG Tru9I 448 TTAA T^TAA 1496 TTAA Tsp509I 58 AATT ^AATT 383 AATT XcmI 269 CCACCTTCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 995 CCATGAACCTCCTGG XhoI 1175 CTCGAG C^TCGAG 1411 CTCGAG XmaCI 1019 CCCGGG C^CCGGG 1592 CCCGGG XmaI 1019 CCCGGG C^CCGGG 1592 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AfaI AluI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI TseI Tsp45I TspRI TthHB8I