(Circular) (Six-base) MAPSORT of: pFN21AE5772.seq Check: 6559 from: 1 to: 6484 pFN21AE5772 6484 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3525 3525 Size: 6484 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 3613 3805 3613 Size: 192 6292 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 2310 2480 3230 4547 5931 6199 1632 Size: 596 82 170 750 1317 1384 268 1917 Fragments arranged by size: 1917 1384 1317 750 596 268 170 82 AfeI AGC'GCT Cuts at: 2130 2130 Size: 6484 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 5383 6360 1203 Size: 4180 977 1327 Fragments arranged by size: 4180 1327 977 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3362 1184 Size: 2178 4306 AlwNI CAG_nnn'CTG Cuts at: 2267 3319 4227 5799 2267 Size: 1052 908 1572 2952 Fragments arranged by size: 2952 1572 1052 908 ApaLI G'TGCA_C Cuts at: 3726 4541 5697 3726 Size: 815 1156 4513 Fragments arranged by size: 4513 1156 815 ApoI r'AATT_y Cuts at: 1493 3793 4019 4171 6280 1493 Size: 2300 226 152 2109 1697 Fragments arranged by size: 2300 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 2702 3024 3180 3416 3450 3597 3809 Size: 73 759 322 156 236 34 147 212 Cuts at: 3809 1870 Size: 4545 Fragments arranged by size: 4545 759 322 236 212 156 147 73 34 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2669 3338 3613 3625 3805 Size: 325 217 627 882 669 275 12 180 Cuts at: 3805 5260 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 882 669 627 325 275 217 180 12 BanII G_rGCy'C Cuts at: 1391 2301 2688 3434 3636 3803 1391 Size: 910 387 746 202 167 4072 Fragments arranged by size: 4072 910 746 387 202 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2743 928 Size: 971 844 4669 Fragments arranged by size: 4669 971 844 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 3195 1548 Size: 1647 4837 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2940 3044 5095 Size: 107 122 71 631 605 1268 104 2051 Cuts at: 5095 136 Size: 1525 Fragments arranged by size: 2051 1525 1268 631 605 122 107 104 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 2867 3901 2867 Size: 1034 5450 Bme1580I G_kGCm'C Cuts at: 1790 2672 3288 3730 3745 4545 5701 1790 Size: 882 616 442 15 800 1156 2573 Fragments arranged by size: 2573 1156 882 800 616 442 15 BmgBI CAC'GTC Cuts at: 1318 3748 1318 Size: 2430 4054 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4215 5173 442 Size: 416 53 83 399 24 2798 958 1753 Fragments arranged by size: 2798 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3476 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 2555 3032 3668 5144 1239 Size: 1253 63 477 636 1476 2579 Fragments arranged by size: 2579 1476 1253 636 477 63 Bpu10I CC'TnA_GC Cuts at: 3428 3428 Size: 6484 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3446 4609 5474 5772 3446 Size: 1163 865 298 4158 Fragments arranged by size: 4158 1163 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3644 5147 882 Size: 617 2145 1503 2219 Fragments arranged by size: 2219 2145 1503 617 BsaAI yAC'GTr Cuts at: 493 1204 3307 3389 4275 493 Size: 711 2103 82 886 2702 Fragments arranged by size: 2702 2103 886 711 82 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 4291 4673 5308 Size: 53 83 186 936 501 2257 382 635 Cuts at: 5308 275 Size: 1451 Fragments arranged by size: 2257 1451 936 635 501 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4917 5315 5589 5736 6147 4917 Size: 398 274 147 411 5254 Fragments arranged by size: 5254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2489 2519 1178 Size: 30 165 30 1086 30 5143 Fragments arranged by size: 5143 1086 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2510 1186 Size: 1324 5160 BseYI C'CCAG_C Cuts at: 2308 2665 2738 2874 3047 5687 6183 2308 Size: 357 73 136 173 2640 496 2609 Fragments arranged by size: 2640 2609 496 357 173 136 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2996 3452 1163 Size: 573 1260 456 4195 Fragments arranged by size: 4195 1260 573 456 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3780 3934 4695 5723 1540 Size: 275 186 1779 154 761 1028 2301 Fragments arranged by size: 2301 1779 1028 761 275 186 154 BsiHKAI G_wGCw'C Cuts at: 2217 2688 3730 4545 4630 5701 2217 Size: 471 1042 815 85 1071 3000 Fragments arranged by size: 3000 1071 1042 815 471 85 BsmI GAATG_Cn' Cuts at: 3019 3995 4088 3019 Size: 976 93 5415 Fragments arranged by size: 5415 976 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2480 2480 Size: 6484 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2301 2672 2688 3288 3434 3636 Size: 399 427 84 371 16 600 146 202 Cuts at: 3636 3730 3745 3803 4545 4630 5701 1391 Size: 94 15 58 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 600 427 399 371 202 146 94 85 84 58 16 15 BspEI T'CCGG_A Cuts at: 6147 6147 Size: 6484 BspHI T'CATG_A Cuts at: 3171 4373 6103 3171 Size: 1202 1730 3552 Fragments arranged by size: 3552 1730 1202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2260 3384 3414 3525 3839 844 Size: 1416 1124 30 111 314 3489 Fragments arranged by size: 3489 1416 1124 314 111 30 BsrBI CCG'CTC Cuts at: 1394 1823 3328 4371 1394 Size: 429 1505 1043 3507 Fragments arranged by size: 3507 1505 1043 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2059 2815 4979 5153 65 Size: 1183 811 756 2164 174 1396 Fragments arranged by size: 2164 1396 1183 811 756 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3141 5128 5315 1700 Size: 110 1331 1987 187 2869 Fragments arranged by size: 2869 1987 1331 187 110 BsrGI T'GTAC_A Cuts at: 96 3570 96 Size: 3474 3010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 4544 5556 4544 Size: 1012 5472 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 2066 5288 2066 Size: 3222 3262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstYI r'GATC_y Cuts at: 1972 3204 3814 4181 4566 4583 6035 6121 6133 Size: 1232 610 367 385 17 1452 86 12 Cuts at: 6133 6479 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1232 610 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2796 3474 513 Size: 552 939 792 678 3523 Fragments arranged by size: 3523 939 792 678 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 3126 3228 3317 4000 4794 4814 3126 Size: 102 89 683 794 20 4796 Fragments arranged by size: 4796 794 683 102 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DraIII CAC_nnn'GTG Cuts at: 3475 3475 Size: 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2097 2767 Size: 54 1425 50 275 18 177 90 670 Cuts at: 2767 3054 3139 3777 3931 4820 6449 8 Size: 287 85 638 154 889 1629 43 Fragments arranged by size: 1629 1425 889 670 638 287 275 177 154 90 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3777 3931 1537 Size: 275 1965 154 4090 Fragments arranged by size: 4090 1965 275 154 EarI CTCTTCn'nnn_ Cuts at: 1353 2827 4414 1353 Size: 1474 1587 3423 Fragments arranged by size: 3423 1587 1474 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5071 5445 5591 387 Size: 1064 138 3482 374 146 1280 Fragments arranged by size: 3482 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2686 2686 Size: 6484 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2310 2480 2492 2555 3032 3230 Size: 393 596 82 170 12 63 477 198 Cuts at: 3230 3668 4547 5144 5931 6199 1239 Size: 438 879 597 787 268 1524 Fragments arranged by size: 1524 879 787 597 596 477 438 393 268 198 170 82 63 12 EcoO109I rG'GnC_Cy Cuts at: 2176 2330 2905 3028 3088 3251 3412 3484 2176 Size: 154 575 123 60 163 161 72 5176 Fragments arranged by size: 5176 575 163 161 154 123 72 60 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3634 3634 Size: 6484 FspI TGC'GCA Cuts at: 2082 4990 2082 Size: 2908 3576 FspAI rTGC'GCAy Cuts at: 2082 2082 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 2132 2874 3584 5631 1678 Size: 454 742 710 2047 2531 Fragments arranged by size: 2531 2047 742 710 454 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2296 2328 2489 2521 2636 Size: 32 135 32 724 32 161 32 115 Cuts at: 2636 2668 5125 5157 5199 5231 1373 Size: 32 2457 32 42 32 2626 Fragments arranged by size: 2626 2457 724 161 135 115 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3751 3828 4074 669 Size: 988 281 1813 77 246 3079 Fragments arranged by size: 3079 1813 988 281 246 77 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3617 3809 3617 Size: 192 6292 MfeI C'AATT_G Cuts at: 2387 4083 2387 Size: 1696 4788 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2055 2601 5598 5782 1305 Size: 750 546 2997 184 2007 Fragments arranged by size: 2997 2007 750 546 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 2769 3056 10 Size: 54 1425 343 267 670 287 3438 Fragments arranged by size: 3438 1425 670 343 287 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2148 2609 3344 3698 4442 4801 4960 Size: 648 982 461 735 354 744 359 159 Cuts at: 4960 518 Size: 2042 Fragments arranged by size: 2042 982 744 735 648 461 359 354 159 MspA1I CmG'CkG Cuts at: 1606 2012 2264 2533 2798 3900 4577 5725 5970 Size: 406 252 269 265 1102 677 1148 245 Cuts at: 5970 1606 Size: 2120 Fragments arranged by size: 2120 1148 1102 677 406 269 265 252 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4993 Fragments arranged by size: 4993 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3777 3931 3777 Size: 154 6330 NspI r_CATG'y Cuts at: 1132 1229 3757 3842 5387 1132 Size: 97 2528 85 1545 2229 Fragments arranged by size: 2528 2229 1545 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 2881 1108 Size: 1773 4711 PfoI T'CCnGG_A Cuts at: 2481 3009 2481 Size: 528 5956 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 2905 3251 2176 Size: 729 346 5409 Fragments arranged by size: 5409 729 346 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspXI vC'TCGA_Gb Cuts at: 3180 3416 3180 Size: 236 6248 PstI C_TGCA'G Cuts at: 830 2532 3036 3411 3522 3836 830 Size: 1702 504 375 111 314 3478 Fragments arranged by size: 3478 1702 504 375 314 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2264 2533 2264 Size: 269 6215 SacI G_AGCT'C Cuts at: 2688 2688 Size: 6484 SacII CC_GC'GG Cuts at: 2799 2799 Size: 6484 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SanDI GG'GwC_CC Cuts at: 3251 3251 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3036 3411 3836 3036 Size: 375 425 5684 Fragments arranged by size: 5684 425 375 ScaI AGT'ACT Cuts at: 2855 4732 6444 2855 Size: 1877 1712 2895 Fragments arranged by size: 2895 1877 1712 SfcI C'TryA_G Cuts at: 826 1045 2528 3032 3407 3518 3832 4967 5648 Size: 219 1483 504 375 111 314 1135 681 Cuts at: 5648 5839 826 Size: 191 1471 Fragments arranged by size: 1483 1471 1135 681 504 375 314 219 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3026 3599 3811 1872 Size: 1154 573 212 4545 Fragments arranged by size: 4545 1154 573 212 SmlI C'TyrA_G Cuts at: 820 1017 1943 3180 3416 3461 4588 5489 5751 Size: 197 926 1237 236 45 1127 901 262 Cuts at: 5751 820 Size: 1553 Fragments arranged by size: 1553 1237 1127 926 901 262 236 197 45 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3842 1132 Size: 2710 3774 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 2708 1517 Size: 1191 5293 StyI C'CwwG_G Cuts at: 513 1065 2004 2179 3487 3923 513 Size: 552 939 175 1308 436 3074 Fragments arranged by size: 3074 1308 939 552 436 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 2454 2939 2963 4511 4528 4681 Size: 1255 672 202 485 24 1548 17 153 Cuts at: 4681 4866 5311 325 Size: 185 445 1498 Fragments arranged by size: 1548 1498 1255 672 485 445 202 185 153 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2853 3570 4730 Size: 274 80 33 51 1443 876 717 1160 Cuts at: 4730 6442 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1160 876 717 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2214 2246 3231 3263 6444 6476 2214 Size: 32 985 32 3181 32 2222 Fragments arranged by size: 3181 2222 985 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 3007 2281 Size: 726 5758 XhoI C'TCGA_G Cuts at: 1943 3180 3416 1943 Size: 1237 236 5011 Fragments arranged by size: 5011 1237 236 XmaI C'CCGG_G Cuts at: 1870 3024 3597 3809 1870 Size: 1154 573 212 4545 Fragments arranged by size: 4545 1154 573 212 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI