Restriction map for
pFN21AE5772
(Linear) (Six-base) MAPSORT of: KIEE5772.seq Check: 6512 from: 1 to: 1779
KIEE5772.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1520 1779
Size: 1520 259
Acc65I G'GTAC_C
Cuts at: 0 1608 1779
Size: 1608 171
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 305 475 1225 1779
Size: 223 82 170 750 554
Fragments arranged by size:
750 554 223 170 82
AfeI AGC'GCT
Cuts at: 0 125 1779
Size: 125 1654
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1357 1779
Size: 1357 422
AlwNI CAG_nnn'CTG
Cuts at: 0 262 1314 1779
Size: 262 1052 465
Fragments arranged by size:
1052 465 262
ApaLI G'TGCA_C
Cuts at: 0 1721 1779
Size: 1721 58
AvaI C'yCGr_G
Cuts at: 0 697 1019 1175 1411 1445 1592 1779
Size: 697 322 156 236 34 147 187
Fragments arranged by size:
697 322 236 187 156 147 34
BanI G'GyrC_C
Cuts at: 0 664 1333 1608 1620 1779
Size: 664 669 275 12 159
Fragments arranged by size:
669 664 275 159 12
BanII G_rGCy'C
Cuts at: 0 296 683 1429 1631 1779
Size: 296 387 746 202 148
Fragments arranged by size:
746 387 296 202 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 738 1779
Size: 738 1041
BclI T'GATC_A
Cuts at: 0 1190 1779
Size: 1190 589
BglI GCCn_nnn'nGGC
Cuts at: 0 935 1039 1779
Size: 935 104 740
Fragments arranged by size:
935 740 104
BlpI GC'TnA_GC
Cuts at: 0 862 1779
Size: 862 917
Bme1580I G_kGCm'C
Cuts at: 0 667 1283 1725 1740 1779
Size: 667 616 442 15 39
Fragments arranged by size:
667 616 442 39 15
BmgBI CAC'GTC
Cuts at: 0 1743 1779
Size: 1743 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1471 1779
Size: 1471 308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 550 1027 1663 1779
Size: 487 63 477 636 116
Fragments arranged by size:
636 487 477 116 63
Bpu10I CC'TnA_GC
Cuts at: 0 1423 1779
Size: 1423 356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1441 1779
Size: 1441 338
BsaI GGTCTCn'nnnn_
Cuts at: 0 1639 1779
Size: 1639 140
BsaAI yAC'GTr
Cuts at: 0 1302 1384 1779
Size: 1302 82 395
Fragments arranged by size:
1302 395 82
BsaHI Gr'CG_yC
Cuts at: 0 29 1779
Size: 29 1750
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 484 514 1779
Size: 484 30 1265
Fragments arranged by size:
1265 484 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 1779
Size: 505 1274
BseYI C'CCAG_C
Cuts at: 0 303 660 733 869 1042 1779
Size: 303 357 73 136 173 737
Fragments arranged by size:
737 357 303 173 136 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1447 1779
Size: 991 456 332
Fragments arranged by size:
991 456 332
BsiEI CG_ry'CG
Cuts at: 0 1775 1779
Size: 1775 4
BsiHKAI G_wGCw'C
Cuts at: 0 212 683 1725 1779
Size: 212 471 1042 54
Fragments arranged by size:
1042 471 212 54
BsmI GAATG_Cn'
Cuts at: 0 1014 1779
Size: 1014 765
BsmBI CGTCTCn'nnnn_
Cuts at: 0 475 1779
Size: 475 1304
Bsp1286I G_dGCh'C
Cuts at: 0 212 296 667 683 1283 1429 1631 1725
Size: 212 84 371 16 600 146 202 94
Cuts at: 1725 1740 1779
Size: 15 39
Fragments arranged by size:
600 371 212 202 146 94 84 39
16 15
BspHI T'CATG_A
Cuts at: 0 1166 1779
Size: 1166 613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 255 1379 1409 1520 1779
Size: 255 1124 30 111 259
Fragments arranged by size:
1124 259 255 111 30
BsrBI CCG'CTC
Cuts at: 0 1323 1779
Size: 1323 456
BsrDI GCAATG_nn'
Cuts at: 0 54 810 1779
Size: 54 756 969
Fragments arranged by size:
969 756 54
BsrFI r'CCGG_y
Cuts at: 0 1136 1779
Size: 1136 643
BsrGI T'GTAC_A
Cuts at: 0 1565 1779
Size: 1565 214
BstBI TT'CG_AA
Cuts at: 0 61 1779
Size: 61 1718
BstYI r'GATC_y
Cuts at: 0 1199 1779
Size: 1199 580
BtgI C'CryG_G
Cuts at: 0 791 1469 1779
Size: 791 678 310
Fragments arranged by size:
791 678 310
BtsI GCAGTG_nn'
Cuts at: 0 1121 1223 1312 1779
Size: 1121 102 89 467
Fragments arranged by size:
1121 467 102 89
DraIII CAC_nnn'GTG
Cuts at: 0 1470 1779
Size: 1470 309
EaeI y'GGCC_r
Cuts at: 0 2 92 762 1049 1134 1772 1779
Size: 2 90 670 287 85 638 7
Fragments arranged by size:
670 638 287 90 85 7 2
EagI C'GGCC_G
Cuts at: 0 1772 1779
Size: 1772 7
EarI CTCTTCn'nnn_
Cuts at: 0 822 1779
Size: 822 957
EcoICRI GAG'CTC
Cuts at: 0 681 1779
Size: 681 1098
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 305 475 487 550 1027 1225 1663
Size: 223 82 170 12 63 477 198 438
Cuts at: 1663 1779
Size: 116
Fragments arranged by size:
477 438 223 198 170 116 82 63
12
EcoO109I rG'GnC_Cy
Cuts at: 0 171 325 900 1023 1083 1246 1407 1479
Size: 171 154 575 123 60 163 161 72
Cuts at: 1479 1779
Size: 300
Fragments arranged by size:
575 300 171 163 161 154 123 72
60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1629 1779
Size: 1629 150
FspI TGC'GCA
Cuts at: 0 77 1779
Size: 77 1702
FspAI rTGC'GCAy
Cuts at: 0 77 1779
Size: 77 1702
HaeII r_GCGC'y
Cuts at: 0 127 869 1579 1779
Size: 127 742 710 200
Fragments arranged by size:
742 710 200 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 291 323 484 516 631 663 1779
Size: 291 32 161 32 115 32 1116
Fragments arranged by size:
1116 291 161 115 32 32 32
HincII GTy'rAC
Cuts at: 0 1746 1779
Size: 1746 33
KpnI G_GTAC'C
Cuts at: 0 1612 1779
Size: 1612 167
MfeI C'AATT_G
Cuts at: 0 382 1779
Size: 382 1397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 596 1779
Size: 50 546 1183
Fragments arranged by size:
1183 546 50
MscI TGG'CCA
Cuts at: 0 94 764 1051 1779
Size: 94 670 287 728
Fragments arranged by size:
728 670 287 94
MslI CAynn'nnrTG
Cuts at: 0 143 604 1339 1693 1779
Size: 143 461 735 354 86
Fragments arranged by size:
735 461 354 143 86
MspA1I CmG'CkG
Cuts at: 0 7 259 528 793 1779
Size: 7 252 269 265 986
Fragments arranged by size:
986 269 265 252 7
NotI GC'GGCC_GC
Cuts at: 0 1772 1779
Size: 1772 7
NspI r_CATG'y
Cuts at: 0 1752 1779
Size: 1752 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 876 1779
Size: 876 903
PfoI T'CCnGG_A
Cuts at: 0 476 1004 1779
Size: 476 528 775
Fragments arranged by size:
775 528 476
PpuMI rG'GwC_Cy
Cuts at: 0 171 900 1246 1779
Size: 171 729 346 533
Fragments arranged by size:
729 533 346 171
PspXI vC'TCGA_Gb
Cuts at: 0 1175 1411 1779
Size: 1175 236 368
Fragments arranged by size:
1175 368 236
PstI C_TGCA'G
Cuts at: 0 527 1031 1406 1517 1779
Size: 527 504 375 111 262
Fragments arranged by size:
527 504 375 262 111
PvuII CAG'CTG
Cuts at: 0 259 528 1779
Size: 259 269 1251
Fragments arranged by size:
1251 269 259
SacI G_AGCT'C
Cuts at: 0 683 1779
Size: 683 1096
SacII CC_GC'GG
Cuts at: 0 794 1779
Size: 794 985
SanDI GG'GwC_CC
Cuts at: 0 1246 1779
Size: 1246 533
SbfI CC_TGCA'GG
Cuts at: 0 1031 1406 1779
Size: 1031 375 373
Fragments arranged by size:
1031 375 373
ScaI AGT'ACT
Cuts at: 0 850 1779
Size: 850 929
SfcI C'TryA_G
Cuts at: 0 523 1027 1402 1513 1779
Size: 523 504 375 111 266
Fragments arranged by size:
523 504 375 266 111
SmaI CCC'GGG
Cuts at: 0 1021 1594 1779
Size: 1021 573 185
Fragments arranged by size:
1021 573 185
SmlI C'TyrA_G
Cuts at: 0 1175 1411 1456 1779
Size: 1175 236 45 323
Fragments arranged by size:
1175 323 236 45
StuI AGG'CCT
Cuts at: 0 703 1779
Size: 703 1076
StyI C'CwwG_G
Cuts at: 0 174 1482 1779
Size: 174 1308 297
Fragments arranged by size:
1308 297 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 449 934 958 1779
Size: 247 202 485 24 821
Fragments arranged by size:
821 485 247 202 24
TatI w'GTAC_w
Cuts at: 0 848 1565 1779
Size: 848 717 214
Fragments arranged by size:
848 717 214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 209 241 1226 1258 1779
Size: 209 32 985 32 521
Fragments arranged by size:
985 521 209 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 1002 1779
Size: 276 726 777
Fragments arranged by size:
777 726 276
XhoI C'TCGA_G
Cuts at: 0 1175 1411 1779
Size: 1175 236 368
Fragments arranged by size:
1175 368 236
XmaI C'CCGG_G
Cuts at: 0 1019 1592 1779
Size: 1019 573 187
Fragments arranged by size:
1019 573 187
Enzymes that do cut:
AarI Acc65I AcuI AfeI AlfI AlwNI ApaLI AvaI
BanI BanII BbsI BclI BglI BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstYI
BtgI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII KpnI
MfeI MmeI MscI MslI MspA1I NotI NspI PflMI
PfoI PpuMI PspXI PstI PvuII SacI SacII SanDI
SbfI ScaI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AleI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BfrBI BglII BmrI
BmtI BsaBI BsaWI BsiWI BspEI BssHII BssSI BstAPI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EciI EcoNI EcoRI EcoRV FseI HindIII HpaI
KasI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SalI SapI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XmnI ZraI