################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MaeIII MfeI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp45I Tsp509I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AcyI 164 GACGCC G[GA]^CG[CT]C AspHI 132 GAGCTC G[AT]GC[AT]^C BamHI 488 GGATCC G^GATCC BanI 612 GGCACC G^G[CT][GA]CC BclI 386 TGATCA T^GATCA BsaBI 657 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 164 GACGCC G[GA]^CG[CT]C BsaJI 312 CCTTGG C^C[ACGT][ACGT]GG BsgI 553 CTGCAC GTGCAG(16/14) BsiHKAI 132 GAGCTC G[AT]GC[AT]^C BsmI 344 GCATTC GAATGC(1/-1) Bsp1407I 641 TGTACA T^GTACA BsrGI 641 TGTACA T^GTACA BsrI 674 ACTGG ACTGG(1/-1) Cac8I 555 GCACGC GC[ACGT]^[ACGT]GC EcoT14I 312 CCTTGG C^C[AT][AT]GG FbaI 386 TGATCA T^GATCA HaeIII 628 GGCC GG^CC HgaI 164 GACGC GACGC(5/10) Hin1I 164 GACGCC G[GA]^CG[CT]C HphI 49 GGTGA GGTGA(8/7) MamI 657 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 345 CATTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 645 CATG CATG^ NspI 644 ACATGC [GA]CATG^[CT] SacI 132 GAGCTC GAGCT^C SfaNI 189 GATGC GCATC(5/9) SmlI 334 CTCAAG C^T[CT][GA]AG SspBI 641 TGTACA T^GTACA StyI 312 CCTTGG C^C[AT][AT]GG TaqI 659 TCGA T^CGA TspRI 183 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 659 TCGA T^CGA XcmI 422 CCAAACAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AfaI 399 GTAC GT^AC 642 GTAC Asp700I 204 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 228 GAAGTTGTTC AspI 114 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 245 GACTGAGTC BfaI 199 CTAG C^TAG 695 CTAG MaeI 199 CTAG C^TAG 695 CTAG PflFI 114 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 245 GACTGAGTC PvuII 253 CAGCTG CAG^CTG 701 CAGCTG RsaI 399 GTAC GT^AC 642 GTAC TfiI 52 GATTC G^A[AT]TC 339 GATTC Tth111I 114 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 245 GACTGAGTC XmnI 204 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 228 GAAGTTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanII BbvI BmyI BpmI BseRI BsiYI BslI BsmFI Bsp1286I BsrBI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HinfI ItaI Ksp632I MboI MboII MflI MnlI MspA1I MvaI MwoI NdeII NlaIV PflMI PleI PstI Sau3AI Sau96I ScrFI SfcI TseI Van91I XhoII