Restriction map for
pFN21AE9285
(Circular) (Six-base) MAPSORT of: pFN21AE9285.seq Check: 5454 from: 1 to: 5443
pFN21AE9285 5443 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3506 4890 5158 1632
Size: 1874 1384 268 1917
Fragments arranged by size:
1917 1874 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5443
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 2222
Size: 32 5411
AlwNI CAG_nnn'CTG
Cuts at: 2437 2503 2524 3186 4758 2437
Size: 66 21 662 1572 3122
Fragments arranged by size:
3122 1572 662 66 21
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2752 2978 3130 5239 1493
Size: 1259 226 152 2109 1697
Fragments arranged by size:
2109 1697 1259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2768 1870
Size: 73 825 4545
Fragments arranged by size:
4545 825 73
BamHI G'GATC_C
Cuts at: 2493 2773 3140 2493
Size: 280 367 4796
Fragments arranged by size:
4796 367 280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2617 2764 4219 618
Size: 325 217 627 830 147 1455 1842
Fragments arranged by size:
1842 1455 830 627 325 217 147
BanII G_rGCy'C
Cuts at: 1391 2141 2299 2316 2762 1391
Size: 750 158 17 446 4072
Fragments arranged by size:
4072 750 446 158 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3634 3668 1903
Size: 34 50 34 1613 34 3678
Fragments arranged by size:
3678 1613 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 2391 1548
Size: 843 4600
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4054 136
Size: 107 122 71 631 605 2382 1525
Fragments arranged by size:
2382 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 3504 4660 1790
Size: 1714 1156 2573
Fragments arranged by size:
2573 1714 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2459 2525 2560 2693 4103 1239
Size: 1055 165 66 35 133 1410 2579
Fragments arranged by size:
2579 1410 1055 165 133 66 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 3568 4433 4731 2324
Size: 1244 865 298 3036
Fragments arranged by size:
3036 1244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4106 882
Size: 617 2607 2219
Fragments arranged by size:
2607 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 2666 5437 2666
Size: 2771 2672
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2170 3250 3632 4267
Size: 53 83 186 936 637 1080 382 635
Cuts at: 4267 275
Size: 1451
Fragments arranged by size:
1451 1080 936 637 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3876 4274 4548 4695 5106 3876
Size: 398 274 147 411 4213
Fragments arranged by size:
4213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2222 2252 1178
Size: 30 165 30 819 30 4369
Fragments arranged by size:
4369 819 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2111 2114 2147 2522 1186
Size: 910 15 3 33 375 4107
Fragments arranged by size:
4107 910 375 33 15 3
BseYI C'CCAG_C
Cuts at: 2600 4646 5142 2600
Size: 2046 496 2901
Fragments arranged by size:
2901 2046 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2543 1163
Size: 573 807 4063
Fragments arranged by size:
4063 807 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2141 3504 3589 4660 2141
Size: 1363 85 1071 2924
Fragments arranged by size:
2924 1363 1071 85
BsmI GAATG_Cn'
Cuts at: 2349 2954 3047 2349
Size: 605 93 4745
Fragments arranged by size:
4745 605 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2299 2316 2762 3504 3589 4660
Size: 399 351 158 17 446 742 85 1071
Cuts at: 4660 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 446 399 351 158 85
17
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 844
Size: 1954 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 2109 2491 3330 1394
Size: 429 281 5 382 839 3507
Fragments arranged by size:
3507 839 429 382 281 5
BsrDI GCAATG_nn'
Cuts at: 65 1248 3938 4112 65
Size: 1183 2690 174 1396
Fragments arranged by size:
2690 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4087 4274 1700
Size: 110 2277 187 2869
Fragments arranged by size:
2869 2277 187 110
BsrGI T'GTAC_A
Cuts at: 96 2646 96
Size: 2550 2893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2364 2493 2527 2773 3140 3525 3542 4994
Size: 392 129 34 246 367 385 17 1452
Cuts at: 4994 5080 5092 5438 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 392 385 367 346 246 129
86 34 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4891
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4704
Fragments arranged by size:
4704 683 56
BtsI GCAGTG_nn'
Cuts at: 2959 3753 3773 2959
Size: 794 20 4629
Fragments arranged by size:
4629 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2890 3779 5408
Size: 54 1425 50 275 18 1060 889 1629
Cuts at: 5408 8
Size: 43
Fragments arranged by size:
1629 1425 1060 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2082 2124 2717 3373 1353
Size: 678 51 42 593 656 3423
Fragments arranged by size:
3423 678 656 593 51 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4030 4404 4550 387
Size: 1064 138 2441 374 146 1280
Fragments arranged by size:
2441 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2139 2139
Size: 5443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2459 2525 2560 2693 3506 4103
Size: 393 662 165 66 35 133 813 597
Cuts at: 4103 4890 5158 1239
Size: 787 268 1524
Fragments arranged by size:
1524 813 787 662 597 393 268 165
133 66 35
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 4590 1678
Size: 2912 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2512 2519 2544 2551 4084
Size: 32 135 32 940 7 25 7 1533
Cuts at: 4084 4116 4158 4190 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1533 940 135 42 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3033 669
Size: 988 281 849 246 3079
Fragments arranged by size:
3079 988 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4557 4741 1305
Size: 3252 184 2007
Fragments arranged by size:
3252 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3621
Fragments arranged by size:
3621 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 3401 3760 3919 518
Size: 648 1188 1047 359 159 2042
Fragments arranged by size:
2042 1188 1047 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2260 2332 2708 2859 3536 4684 4929 1606
Size: 654 72 376 151 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 654 376 245 151 72
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4891
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2653 2801 4346 1132
Size: 97 1424 148 1545 2229
Fragments arranged by size:
2229 1545 1424 148 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5443
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2503 2524 2583 1108
Size: 1395 21 59 3968
Fragments arranged by size:
3968 1395 59 21
PfoI T'CCnGG_A
Cuts at: 2670 2670
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2198 2222 2230 2254 3512 3544 5025 5057 2198
Size: 24 8 24 1258 32 1481 32 2584
Fragments arranged by size:
2584 1481 1258 32 32 24 24 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PstI C_TGCA'G
Cuts at: 830 2331 2421 2475 2487 2795 830
Size: 1501 90 54 12 308 3478
Fragments arranged by size:
3478 1501 308 90 54 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
PvuII CAG'CTG
Cuts at: 2260 2708 2260
Size: 448 4995
SacI G_AGCT'C
Cuts at: 2141 2141
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SfcI C'TryA_G
Cuts at: 826 1045 2327 2417 2471 2483 2791 3926 4607
Size: 219 1282 90 54 12 308 1135 681
Cuts at: 4607 4798 826
Size: 191 1471
Fragments arranged by size:
1471 1282 1135 681 308 219 191 90
54 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2770 1872
Size: 898 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 3547 4448 4710 820
Size: 197 926 396 1208 901 262 1553
Fragments arranged by size:
1553 1208 926 901 396 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2317 2882 513
Size: 552 1252 565 3074
Fragments arranged by size:
3074 1252 565 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3470 3487 3640 3825 4270 325
Size: 1255 1890 17 153 185 445 1498
Fragments arranged by size:
1890 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2646 3689 5401
Size: 274 80 33 51 1443 669 1043 1712
Cuts at: 5401 96
Size: 138
Fragments arranged by size:
1712 1443 1043 669 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5403 5435 5403
Size: 32 5411
Tth111I GACn'n_nGTC
Cuts at: 1316 2122 2253 1316
Size: 806 131 4506
Fragments arranged by size:
4506 806 131
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2434
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2768 1870
Size: 898 4545
XmnI GAAnn'nnTTC
Cuts at: 2213 2237 3572 2213
Size: 24 1335 4084
Fragments arranged by size:
4084 1335 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI