Restriction map for
pFN21AE9285
(Linear) (Six-base) MAPSORT of: KIEE9285.seq Check: 5387 from: 1 to: 738
KIEE9285.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 738
Size: 217 32 489
Fragments arranged by size:
489 217 32
AlwNI CAG_nnn'CTG
Cuts at: 0 432 498 519 738
Size: 432 66 21 219
Fragments arranged by size:
432 219 66 21
BamHI G'GATC_C
Cuts at: 0 488 738
Size: 488 250
BanI G'GyrC_C
Cuts at: 0 612 738
Size: 612 126
BanII G_rGCy'C
Cuts at: 0 136 294 311 738
Size: 136 158 17 427
Fragments arranged by size:
427 158 136 17
BclI T'GATC_A
Cuts at: 0 386 738
Size: 386 352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 150 738
Size: 150 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 454 520 555 688 738
Size: 289 165 66 35 133 50
Fragments arranged by size:
289 165 133 66 50 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 738
Size: 319 419
BsaBI GATnn'nnATC
Cuts at: 0 661 738
Size: 661 77
BsaHI Gr'CG_yC
Cuts at: 0 165 738
Size: 165 573
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 738
Size: 217 30 491
Fragments arranged by size:
491 217 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 106 109 142 517 738
Size: 91 15 3 33 375 221
Fragments arranged by size:
375 221 91 33 15 3
BseYI C'CCAG_C
Cuts at: 0 595 738
Size: 595 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 738
Size: 538 200
BsiHKAI G_wGCw'C
Cuts at: 0 136 738
Size: 136 602
BsmI GAATG_Cn'
Cuts at: 0 344 738
Size: 344 394
Bsp1286I G_dGCh'C
Cuts at: 0 136 294 311 738
Size: 136 158 17 427
Fragments arranged by size:
427 158 136 17
BsrBI CCG'CTC
Cuts at: 0 99 104 486 738
Size: 99 5 382 252
Fragments arranged by size:
382 252 99 5
BsrGI T'GTAC_A
Cuts at: 0 641 738
Size: 641 97
BstYI r'GATC_y
Cuts at: 0 359 488 522 738
Size: 359 129 34 216
Fragments arranged by size:
359 216 129 34
EarI CTCTTCn'nnn_
Cuts at: 0 26 77 119 712 738
Size: 26 51 42 593 26
Fragments arranged by size:
593 51 42 26 26
EcoICRI GAG'CTC
Cuts at: 0 134 738
Size: 134 604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 454 520 555 688 738
Size: 289 165 66 35 133 50
Fragments arranged by size:
289 165 133 66 50 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 507 514 539 546 738
Size: 507 7 25 7 192
Fragments arranged by size:
507 192 25 7 7
MslI CAynn'nnrTG
Cuts at: 0 349 738
Size: 349 389
MspA1I CmG'CkG
Cuts at: 0 255 327 703 738
Size: 255 72 376 35
Fragments arranged by size:
376 255 72 35
NspI r_CATG'y
Cuts at: 0 648 738
Size: 648 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 498 519 578 738
Size: 498 21 59 160
Fragments arranged by size:
498 160 59 21
PfoI T'CCnGG_A
Cuts at: 0 665 738
Size: 665 73
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 193 217 225 249 738
Size: 193 24 8 24 489
Fragments arranged by size:
489 193 24 24 8
PstI C_TGCA'G
Cuts at: 0 326 416 470 482 738
Size: 326 90 54 12 256
Fragments arranged by size:
326 256 90 54 12
PvuII CAG'CTG
Cuts at: 0 255 703 738
Size: 255 448 35
Fragments arranged by size:
448 255 35
SacI G_AGCT'C
Cuts at: 0 136 738
Size: 136 602
SfcI C'TryA_G
Cuts at: 0 322 412 466 478 738
Size: 322 90 54 12 260
Fragments arranged by size:
322 260 90 54 12
SmlI C'TyrA_G
Cuts at: 0 334 738
Size: 334 404
StyI C'CwwG_G
Cuts at: 0 312 738
Size: 312 426
TatI w'GTAC_w
Cuts at: 0 641 738
Size: 641 97
Tth111I GACn'n_nGTC
Cuts at: 0 117 248 738
Size: 117 131 490
Fragments arranged by size:
490 131 117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 738
Size: 429 309
XmnI GAAnn'nnTTC
Cuts at: 0 208 232 738
Size: 208 24 506
Fragments arranged by size:
506 208 24
Enzymes that do cut:
AloI AlwNI BamHI BanI BanII BclI BplI BpmI
BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrBI BsrGI BstYI EarI EcoICRI Eco57MI
Hin4I MslI MspA1I NspI PflMI PfoI PpiI PstI
PvuII SacI SfcI SmlI StyI TatI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI Bpu10I BsaI BsaAI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI XbaI
XhoI XmaI ZraI