################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BamHI BbrPI BbvCI BbvI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HincII HindII HindIII HinfI HpaI ItaI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MseI MslI MspA1I MunI MvnI NaeI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I TseI Tsp45I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AluI 98 AGCT AG^CT AlwNI 142 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 221 CCCGGG C^[CT]CG[GA]G BalI 138 TGGCCA TGG^CCA BanI 202 GGCGCC G^G[CT][GA]CC BanII 37 GAGCCC G[GA]GC[CT]^C BbeI 202 GGCGCC GGCGC^C BbsI 166 GAAGAC GAAGAC(2/6) BcgI 244 CGAGAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 37 GAGCCC G[AGT]GC[ACT]^C BpmI 100 CTGGAG CTGGAG(16/14) BpuAI 166 GAAGAC GAAGAC(2/6) BsmAI 23 GAGAC GTCTC(1/5) BsoBI 221 CCCGGG C^[CT]CG[GA]G Bsp1286I 37 GAGCCC G[AGT]GC[ACT]^C BstF5I 235 GGATG GGATG(2/0) CfoI 203 GCGC GCG^C DsaI 41 CCATGG C^C[GA][CT]GG EaeI 138 TGGCCA [CT]^GGCC[GA] EarI 172 CTCTTC CTCTTC(1/4) FokI 235 GGATG GGATG(9/13) HaeII 202 GGCGCC [GA]GCGC^[CT] HgaI 256 GACGC GACGC(5/10) HhaI 203 GCGC GCG^C HinP1I 203 GCGC G^CGC HphI 79 GGTGA GGTGA(8/7) KasI 202 GGCGCC G^GCGCC Ksp632I 172 CTCTTC CTCTTC(1/4) MluNI 138 TGGCCA TGG^CCA MscI 138 TGGCCA TGG^CCA MwoI 230 GCTTCGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 202 GGCGCC GG^CGCC NcoI 41 CCATGG C^CATGG NlaIII 42 CATG CATG^ PflMI 141 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfaNI 236 GATGC GCATC(5/9) SfoI 202 GGCGCC GGC^GCC SmaI 221 CCCGGG CCC^GGG StuI 122 AGGCCT AGG^CCT TaqI 243 TCGA T^CGA TspRI 56 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 243 TCGA T^CGA Van91I 141 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 221 CCCGGG C^CCGGG XmaI 221 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 202 GGCGCC G[GA]^CG[CT]C 256 GACGCC BsaHI 202 GGCGCC G[GA]^CG[CT]C 256 GACGCC BsiYI 141 CCAGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 171 CCTCTTCCGGG BslI 141 CCAGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 171 CCTCTTCCGGG BsmFI 224 GGGAC GGGAC(10/14) 219 GTCCC BsrI 55 CCAGT ACTGG(1/-1) 141 CCAGT BstNI 66 CCTGG CC^[AT]GG 87 CCTGG EcoRII 66 CCTGG ^CC[AT]GG 87 CCTGG EcoT14I 41 CCATGG C^C[AT][AT]GG 135 CCTTGG HaeIII 123 GGCC GG^CC 139 GGCC HapII 177 CCGG C^CGG 222 CCGG Hin1I 202 GGCGCC G[GA]^CG[CT]C 256 GACGCC HpaII 177 CCGG C^CGG 222 CCGG MboII 166 GAAGA GAAGA(8/7) 173 TCTTC MspI 177 CCGG C^CGG 222 CCGG MvaI 66 CCTGG CC^[AT]GG 87 CCTGG NlaIV 62 GGAGCC GG[ACGT]^[ACGT]CC 202 GGCGCC StyI 41 CCATGG C^C[AT][AT]GG 135 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### BcnI BsaJI BssKI MnlI NciI ScrFI