Restriction map for
pFN21AE5134
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BpmI BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HapII HgaI Hin1I
HindIII HpaII KasI KpnI KspI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 88 GTAC GT^AC
AlwI 185 GGATC GGATC(4/5)
AocI 307 CCTGAGG CC^T[ACGT]AGG
Aor51HI 140 AGCGCT AGC^GCT
BbrPI 274 CACGTG CAC^GTG
BfaI 43 CTAG C^TAG
BlpI 376 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 376 GCTGAGC GC^T[ACGT]AGC
BsaAI 274 CACGTG [CT]AC^GT[GA]
BsiYI 11 CCACCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 11 CCACCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 197 GGGAC GGGAC(10/14)
BspHI 456 TCATGA T^CATGA
BstAPI 51 GCACATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 307 CCTGAGG CC^T[ACGT]AGG
Cac8I 155 GCATGC GC[ACGT]^[ACGT]GC
CelII 376 GCTGAGC GC^T[ACGT]AGC
DpnII 186 GATC ^GATC
DraII 94 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 52 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 140 AGCGCT AGC^GCT
Eco81I 307 CCTGAGG CC^T[ACGT]AGG
EcoO109I 94 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 132 CCAAGG C^C[AT][AT]GG
HaeII 140 AGCGCT [GA]GCGC^[CT]
HpaI 433 GTTAAC GTT^AAC
MaeI 43 CTAG C^TAG
MaeII 275 ACGT A^CGT
MboI 186 GATC ^GATC
MseI 434 TTAA T^TAA
MslI 457 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 374 CAGCTG C[AC]G^C[GT]G
NdeII 186 GATC ^GATC
NlaIV 95 GGACCC GG[ACGT]^[ACGT]CC
PleI 400 GAGTC GAGTC(4/5)
PmaCI 274 CACGTG CAC^GTG
PmlI 274 CACGTG CAC^GTG
PpuMI 94 AGGACCC [GA]G^G[AT]CC[CT]
PstI 286 CTGCAG CTGCA^G
PvuII 374 CAGCTG CAG^CTG
RcaI 456 TCATGA T^CATGA
RsaI 88 GTAC GT^AC
Sau3AI 186 GATC ^GATC
SfcI 286 CTGCAG C^T[GA][CT]AG
SphI 155 GCATGC GCATG^C
StuI 311 AGGCCT AGG^CCT
StyI 132 CCAAGG C^C[AT][AT]GG
TfiI 418 GATTC G^A[AT]TC
Tru9I 434 TTAA T^TAA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 171 CCGC CCGC(-3/-1)
444 CCGC
AluI 375 AGCT AG^CT
406 AGCT
AspHI 49 GAGCAC G[AT]GC[AT]^C
259 GTGCTC
AvaII 95 GGACC G^G[AT]CC
270 GGACC
BsiHKAI 49 GAGCAC G[AT]GC[AT]^C
259 GTGCTC
BsmAI 124 GAGAC GTCTC(1/5)
3 GTCTC
BssKI 100 CCAGG ^CC[ACGT]GG
147 CCTGG
BstNI 100 CCAGG CC^[AT]GG
147 CCTGG
CfoI 141 GCGC GCG^C
279 GCGC
Cfr13I 95 GGACC G^G[ACGT]CC
270 GGACC
EarI 223 GAAGAG CTCTTC(1/4)
478 GAAGAG
EcoRII 100 CCAGG ^CC[AT]GG
147 CCTGG
HhaI 141 GCGC GCG^C
279 GCGC
HinP1I 141 GCGC G^CGC
279 GCGC
HincII 211 GTTGAC GT[CT]^[GA]AC
433 GTTAAC
HindII 211 GTTGAC GT[CT]^[GA]AC
433 GTTAAC
HinfI 400 GAGTC G^A[ACGT]TC
418 GATTC
Ksp632I 223 GAAGAG CTCTTC(1/4)
478 GAAGAG
MvaI 100 CCAGG CC^[AT]GG
147 CCTGG
NspI 24 GCATGT [GA]CATG^[CT]
155 GCATGC
Sau96I 95 GGACC G^G[ACGT]CC
270 GGACC
ScrFI 100 CCAGG CC^[ACGT]GG
147 CCTGG
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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BbvI BmyI BsaJI BseRI Bsp1286I
DdeI Fnu4HI HaeIII HphI ItaI
MboII MnlI MwoI NlaIII TseI