(Linear) (Six-base) MAPSORT of: KIEE5134.seq Check: 864 from: 1 to: 495 KIEE5134.orf With 182 enzymes: SgfI * January 29, 2014 11:47 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 341 495 Size: 341 154 AfeI AGC'GCT Cuts at: 0 142 495 Size: 142 353 BlpI GC'TnA_GC Cuts at: 0 377 495 Size: 377 118 Bme1580I G_kGCm'C Cuts at: 0 62 495 Size: 62 433 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 333 423 495 Size: 333 90 72 Fragments arranged by size: 333 90 72 BsaAI yAC'GTr Cuts at: 0 276 495 Size: 276 219 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 221 251 353 383 495 Size: 221 30 102 30 112 Fragments arranged by size: 221 112 102 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 412 481 484 495 Size: 412 69 3 11 Fragments arranged by size: 412 69 11 3 BseYI C'CCAG_C Cuts at: 0 372 495 Size: 372 123 BsiHKAI G_wGCw'C Cuts at: 0 53 263 495 Size: 53 210 232 Fragments arranged by size: 232 210 53 Bsp1286I G_dGCh'C Cuts at: 0 53 62 263 495 Size: 53 9 201 232 Fragments arranged by size: 232 201 53 9 BspHI T'CATG_A Cuts at: 0 456 495 Size: 456 39 BstAPI GCAn_nnn'nTGC Cuts at: 0 57 495 Size: 57 438 Bsu36I CC'TnA_GG Cuts at: 0 308 495 Size: 308 187 DraIII CAC_nnn'GTG Cuts at: 0 57 495 Size: 57 438 EarI CTCTTCn'nnn_ Cuts at: 0 218 473 495 Size: 218 255 22 Fragments arranged by size: 255 218 22 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 341 495 Size: 341 154 EcoO109I rG'GnC_Cy Cuts at: 0 95 495 Size: 95 400 HaeII r_GCGC'y Cuts at: 0 144 495 Size: 144 351 HincII GTy'rAC Cuts at: 0 213 435 495 Size: 213 222 60 Fragments arranged by size: 222 213 60 HpaI GTT'AAC Cuts at: 0 435 495 Size: 435 60 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 89 495 Size: 89 406 MslI CAynn'nnrTG Cuts at: 0 461 495 Size: 461 34 MspA1I CmG'CkG Cuts at: 0 376 495 Size: 376 119 NspI r_CATG'y Cuts at: 0 28 159 495 Size: 28 131 336 Fragments arranged by size: 336 131 28 PasI CC'CwG_GG Cuts at: 0 100 495 Size: 100 395 PmlI CAC'GTG Cuts at: 0 276 495 Size: 276 219 PpuMI rG'GwC_Cy Cuts at: 0 95 495 Size: 95 400 PstI C_TGCA'G Cuts at: 0 290 495 Size: 290 205 PvuII CAG'CTG Cuts at: 0 376 495 Size: 376 119 SfcI C'TryA_G Cuts at: 0 286 495 Size: 286 209 SphI G_CATG'C Cuts at: 0 159 495 Size: 159 336 StuI AGG'CCT Cuts at: 0 313 495 Size: 313 182 StyI C'CwwG_G Cuts at: 0 132 495 Size: 132 363 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 221 253 495 Size: 221 32 242 Fragments arranged by size: 242 221 32 Enzymes that do cut: AcuI AfeI BlpI Bme1580I BplI BsaAI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI BstAPI Bsu36I DraIII EarI Eco57MI EcoO109I HaeII HincII HpaI MmeI MslI MspA1I NspI PasI PmlI PpuMI PstI PvuII SfcI SphI StuI StyI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI