################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaHI BsaWI BseAI BseRI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI MaeI MamI MboI MfeI MflI MluI MroI MseI MslI MunI NaeI NarI NdeI NdeII NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SalI Sau3AI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 607 GTCTAC GT^[AC][GT]AC AccII 664 CGCG CG^CG Aor51HI 558 AGCGCT AGC^GCT BbrPI 452 CACGTG CAC^GTG BbvCI 278 CCTCAGC CCTCAGC(-5/-2) BsaAI 452 CACGTG [CT]AC^GT[GA] BsaI 231 GGTCTC GGTCTC(1/5) BsgI 676 CTGCAC GTGCAG(16/14) BsmAI 232 GTCTC GTCTC(1/5) BsrDI 424 CATTGC GCAATG(2/0) BsrFI 486 ACCGGC [GA]^CCGG[CT] BssSI 406 CTCGTG CACGAG(-5/-1) BstF5I 303 CATCC GGATG(2/0) BstUI 664 CGCG CG^CG Cfr10I 486 ACCGGC [GA]^CCGG[CT] Eco47III 558 AGCGCT AGC^GCT EcoT14I 225 CCATGG C^C[AT][AT]GG FokI 303 CATCC GGATG(9/13) HaeII 558 AGCGCT [GA]GCGC^[CT] KspI 663 CCGCGG CCGC^GG MaeII 453 ACGT A^CGT MaeIII 483 GTCAC ^GT[ACGT]AC MvnI 664 CGCG CG^CG NcoI 225 CCATGG C^CATGG NotI 665 GCGGCCGC GC^GGCCGC NspI 106 GCATGC [GA]CATG^[CT] PmaCI 452 CACGTG CAC^GTG PmlI 452 CACGTG CAC^GTG PvuII 478 CAGCTG CAG^CTG SacII 663 CCGCGG CCGC^GG SfaNI 302 GCATC GCATC(5/9) SfiI 213 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 106 GCATGC GCATG^C StuI 356 AGGCCT AGG^CCT StyI 225 CCATGG C^C[AT][AT]GG Tsp45I 483 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 85 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 261 CAGGACCTG BcnI 178 CCCGG CC^[GC]GG 658 CCCGG BsiEI 660 CGGCCG CG[GA][CT]^CG 666 CGGCCG BsrI 473 ACTGG ACTGG(1/-1) 519 ACTGG DraIII 679 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 741 CACGGCGTG DsaI 225 CCATGG C^C[GA][CT]GG 663 CCGCGG EagI 660 CGGCCG C^GGCCG 666 CGGCCG EarI 289 CTCTTC CTCTTC(1/4) 190 GAAGAG EclXI 660 CGGCCG C^GGCCG 666 CGGCCG Eco52I 660 CGGCCG C^GGCCG 666 CGGCCG HphI 308 TCACC GGTGA(8/7) 484 TCACC Ksp632I 289 CTCTTC CTCTTC(1/4) 190 GAAGAG MboII 190 GAAGA GAAGA(8/7) 290 TCTTC MspA1I 478 CAGCTG C[AC]G^C[GT]G 663 CCGCGG NciI 178 CCCGG CC^[GC]GG 658 CCCGG PflMI 48 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 84 CCAGGCCCTGG PstI 505 CTGCAG CTGCA^G 588 CTGCAG SacI 124 GAGCTC GAGCT^C 182 GAGCTC SapI 190 GAAGAGC GCTCTTC(1/4) 288 GCTCTTC SfcI 505 CTGCAG C^T[GA][CT]AG 588 CTGCAG Van91I 48 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 84 CCAGGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AluI AspHI AvaII BalI BanII BbvI BglI BlpI BmyI BpmI Bpu1102I BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstXI Cac8I CelII CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MluNI MnlI MscI MspI MvaI MwoI NlaIII NlaIV PpuMI Sau96I ScrFI TseI TspRI