(Circular) (Six-base) MAPSORT of: pFN21AE5290.seq Check: 1435 from: 1 to: 5461 pFN21AE5290 5461 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2613 2804 1937 Size: 676 191 4594 Fragments arranged by size: 4594 676 191 Acc65I G'GTAC_C Cuts at: 2782 2782 Size: 5461 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2105 2567 2608 3524 4908 5176 1632 Size: 473 462 41 916 1384 268 1917 Fragments arranged by size: 1917 1384 916 473 462 268 41 AfeI AGC'GCT Cuts at: 2565 2565 Size: 5461 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5264 AflIII A'CryG_T Cuts at: 1203 4360 5337 1203 Size: 3157 977 1327 Fragments arranged by size: 3157 1327 977 AgeI A'CCGG_T Cuts at: 4292 4292 Size: 5461 AhdI GACnn_n'nnGTC Cuts at: 4190 4190 Size: 5461 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2535 2567 1184 Size: 1351 32 4078 Fragments arranged by size: 4078 1351 32 AlwNI CAG_nnn'CTG Cuts at: 2095 2271 3204 4776 2095 Size: 176 933 1572 2780 Fragments arranged by size: 2780 1572 933 176 ApaLI G'TGCA_C Cuts at: 3518 4674 3518 Size: 1156 4305 ApoI r'AATT_y Cuts at: 1493 2770 2996 3148 5257 1493 Size: 1277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 226 152 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2786 1870 Size: 73 843 4545 Fragments arranged by size: 4545 843 73 BamHI G'GATC_C Cuts at: 2791 3158 2791 Size: 367 5094 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2782 4237 618 Size: 325 217 627 995 1455 1842 Fragments arranged by size: 1842 1455 995 627 325 217 BanII G_rGCy'C Cuts at: 1391 2040 2133 2191 2780 1391 Size: 649 93 58 589 4072 Fragments arranged by size: 4072 649 589 93 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4490 BbvCI CC'TCA_GC Cuts at: 2284 2284 Size: 5461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 1903 Size: 34 1715 34 3678 Fragments arranged by size: 3678 1715 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3353 4563 3353 Size: 1210 4251 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2156 2225 2586 Size: 107 122 71 631 605 484 69 361 Cuts at: 2586 4072 136 Size: 1486 1525 Fragments arranged by size: 1525 1486 631 605 484 361 122 107 71 69 BglII A'GATC_T Cuts at: 5456 5456 Size: 5461 BlpI GC'TnA_GC Cuts at: 2127 2447 2633 2878 2127 Size: 320 186 245 4710 Fragments arranged by size: 4710 320 245 186 Bme1580I G_kGCm'C Cuts at: 1790 2210 2747 3522 4678 1790 Size: 420 537 775 1156 2573 Fragments arranged by size: 2573 1156 775 537 420 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2534 3192 4150 Size: 416 53 83 399 24 1117 658 958 Cuts at: 4150 442 Size: 1753 Fragments arranged by size: 1753 1117 958 658 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2669 2723 4121 1239 Size: 878 552 54 1398 2579 Fragments arranged by size: 2579 1398 878 552 54 Bpu10I CC'TnA_GC Cuts at: 2132 2284 2404 2132 Size: 152 120 5189 Fragments arranged by size: 5189 152 120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3586 4451 4749 3586 Size: 865 298 4298 Fragments arranged by size: 4298 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2242 4124 882 Size: 617 743 1882 2219 Fragments arranged by size: 2219 1882 743 617 BsaAI yAC'GTr Cuts at: 493 1204 2459 3252 493 Size: 711 1255 793 2702 Fragments arranged by size: 2702 1255 793 711 BsaBI GATnn'nnATC Cuts at: 5455 5455 Size: 5461 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3268 3650 4285 275 Size: 53 83 186 936 1735 382 635 1451 Fragments arranged by size: 1735 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3894 4292 4566 4713 5124 3894 Size: 398 274 147 411 4231 Fragments arranged by size: 4231 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5236 Fragments arranged by size: 5236 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5461 BseYI C'CCAG_C Cuts at: 2203 2602 4664 5160 2203 Size: 399 2062 496 2504 Fragments arranged by size: 2504 2062 496 399 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2666 1163 Size: 573 930 3958 Fragments arranged by size: 3958 930 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2668 2674 2911 3672 4700 1540 Size: 275 186 667 6 237 761 1028 2301 Fragments arranged by size: 2301 1028 761 667 275 237 186 6 BsiHKAI G_wGCw'C Cuts at: 2133 2191 2448 3522 3607 4678 2133 Size: 58 257 1074 85 1071 2916 Fragments arranged by size: 2916 1074 1071 257 85 58 BsmI GAATG_Cn' Cuts at: 2972 3065 2972 Size: 93 5368 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2133 2191 2210 2448 2747 2780 Size: 399 250 93 58 19 238 299 33 Cuts at: 2780 3522 3607 4678 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 299 250 238 93 85 58 33 19 BspEI T'CCGG_A Cuts at: 5124 5124 Size: 5461 BspHI T'CATG_A Cuts at: 3350 5080 3350 Size: 1730 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2816 844 Size: 1972 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3348 1394 Size: 429 1525 3507 Fragments arranged by size: 3507 1525 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2428 3956 4130 65 Size: 1183 1180 1528 174 1396 Fragments arranged by size: 1528 1396 1183 1180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2491 4105 4292 1700 Size: 110 681 1614 187 2869 Fragments arranged by size: 2869 1614 681 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5461 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5461 BssSI C'ACGA_G Cuts at: 2411 3521 4533 2411 Size: 1110 1012 3339 Fragments arranged by size: 3339 1110 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5461 BstBI TT'CG_AA Cuts at: 4265 4265 Size: 5461 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5461 BstXI CCAn_nnnn'nTGG Cuts at: 2231 2463 2647 2231 Size: 232 184 5045 Fragments arranged by size: 5045 232 184 BstYI r'GATC_y Cuts at: 1972 2791 3158 3543 3560 5012 5098 5110 5456 Size: 819 367 385 17 1452 86 12 346 Cuts at: 5456 1972 Size: 1977 Fragments arranged by size: 1977 1452 819 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2230 2668 513 Size: 552 939 226 438 3306 Fragments arranged by size: 3306 939 552 438 226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4722 Fragments arranged by size: 4722 683 56 BtsI GCAGTG_nn' Cuts at: 2208 2977 3771 3791 2208 Size: 769 794 20 3878 Fragments arranged by size: 3878 794 769 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5426 DraI TTT'AAA Cuts at: 2765 2765 Size: 5461 DraIII CAC_nnn'GTG Cuts at: 2689 2751 2689 Size: 62 5399 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2217 2329 2415 Size: 54 1425 50 275 18 387 112 86 Cuts at: 2415 2493 2538 2665 2671 2908 3797 5426 8 Size: 78 45 127 6 237 889 1629 43 Fragments arranged by size: 1629 1425 889 387 275 237 127 112 86 78 54 50 45 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2665 2671 2908 1537 Size: 275 853 6 237 4090 Fragments arranged by size: 4090 853 275 237 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 2300 3391 1353 Size: 837 110 1091 3423 Fragments arranged by size: 3423 1091 837 110 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2370 4048 4422 4568 387 Size: 1064 138 781 1678 374 146 1280 Fragments arranged by size: 1678 1280 1064 781 374 146 138 EcoICRI GAG'CTC Cuts at: 2131 2189 2131 Size: 58 5403 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2117 2567 2608 2669 2723 3524 Size: 393 473 12 450 41 61 54 801 Cuts at: 3524 4121 4908 5176 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 801 787 597 473 450 393 268 61 54 41 12 EcoO109I rG'GnC_Cy Cuts at: 2019 2092 2102 2169 2268 2275 2578 2730 2019 Size: 73 10 67 99 7 303 152 4750 Fragments arranged by size: 4750 303 152 99 73 67 10 7 EcoRI G'AATT_C Cuts at: 2770 3148 2770 Size: 378 5083 FspI TGC'GCA Cuts at: 3967 3967 Size: 5461 HaeII r_GCGC'y Cuts at: 1678 2567 4608 1678 Size: 889 2041 2531 Fragments arranged by size: 2531 2041 889 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2267 2299 4102 Size: 32 135 32 450 32 213 32 1803 Cuts at: 4102 4134 4176 4208 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1803 450 213 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3051 669 Size: 988 281 867 246 3079 Fragments arranged by size: 3079 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KpnI G_GTAC'C Cuts at: 2786 2786 Size: 5461 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4575 4759 1305 Size: 3270 184 2007 Fragments arranged by size: 3270 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2219 2331 2417 2540 10 Size: 54 1425 343 387 112 86 123 2931 Fragments arranged by size: 2931 1425 387 343 123 112 86 54 MslI CAynn'nnrTG Cuts at: 518 1166 3419 3778 3937 518 Size: 648 2253 359 159 2042 Fragments arranged by size: 2253 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2485 2670 2877 3554 4702 4947 1606 Size: 879 185 207 677 1148 245 2120 Fragments arranged by size: 2120 1148 879 677 245 207 185 NcoI C'CATG_G Cuts at: 513 1065 2004 2230 513 Size: 552 939 226 3744 Fragments arranged by size: 3744 939 552 226 NdeI CA'TA_TG Cuts at: 387 387 Size: 5461 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2671 2908 2671 Size: 237 5224 NspI r_CATG'y Cuts at: 1132 1229 2115 2819 4364 1132 Size: 97 886 704 1545 2229 Fragments arranged by size: 2229 1545 886 704 97 PasI CC'CwG_GG Cuts at: 1348 2065 2245 2386 1348 Size: 717 180 141 4423 Fragments arranged by size: 4423 717 180 141 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 2059 2095 1108 Size: 951 36 4474 Fragments arranged by size: 4474 951 36 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PmlI CAC'GTG Cuts at: 2459 2459 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2268 2275 2730 2268 Size: 7 455 4999 Fragments arranged by size: 4999 455 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PstI C_TGCA'G Cuts at: 830 2514 2597 2813 830 Size: 1684 83 216 3478 Fragments arranged by size: 3478 1684 216 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 PvuII CAG'CTG Cuts at: 2485 2485 Size: 5461 SacI G_AGCT'C Cuts at: 2133 2191 2133 Size: 58 5403 SacII CC_GC'GG Cuts at: 2671 2671 Size: 5461 SalI G'TCGA_C Cuts at: 1936 2803 1936 Size: 867 4594 SapI GCTCTTCn'nnn_ Cuts at: 2190 2300 2190 Size: 110 5351 SbfI CC_TGCA'GG Cuts at: 2813 2813 Size: 5461 ScaI AGT'ACT Cuts at: 3709 5421 3709 Size: 1712 3749 SfcI C'TryA_G Cuts at: 826 1045 2510 2593 2809 3944 4625 4816 826 Size: 219 1465 83 216 1135 681 191 1471 Fragments arranged by size: 1471 1465 1135 681 219 216 191 83 SfiI GGCCn_nnn'nGGCC Cuts at: 2225 2225 Size: 5461 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5461 SmaI CCC'GGG Cuts at: 1872 2788 1872 Size: 916 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3565 4466 4728 820 Size: 197 926 1622 901 262 1553 Fragments arranged by size: 1622 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3252 493 Size: 2759 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2115 2819 1132 Size: 983 704 3774 Fragments arranged by size: 3774 983 704 SspI AAT'ATT Cuts at: 5 52 3385 5 Size: 47 3333 2081 Fragments arranged by size: 3333 2081 47 StuI AGG'CCT Cuts at: 1517 2363 1517 Size: 846 4615 StyI C'CwwG_G Cuts at: 513 1065 2004 2230 2900 513 Size: 552 939 226 670 3074 Fragments arranged by size: 3074 939 670 552 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2237 3488 3505 3658 3843 4288 325 Size: 1255 657 1251 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1251 657 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3707 5419 96 Size: 274 80 33 51 1443 1730 1712 138 Fragments arranged by size: 1730 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2520 2552 5421 5453 2520 Size: 32 2869 32 2528 Fragments arranged by size: 2869 2528 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5461 XbaI T'CTAG_A Cuts at: 2797 2797 Size: 5461 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5461 XmaI C'CCGG_G Cuts at: 1870 2786 1870 Size: 916 4545 XmnI GAAnn'nnTTC Cuts at: 3590 3590 Size: 5461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SanDI SexAI SfoI SrfI SwaI XcmI