Restriction map for
pFN21AE5290
(Linear) (Six-base) MAPSORT of: KIEE5290.seq Check: 8263 from: 1 to: 756
KIEE5290.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AccI GT'mk_AC
Cuts at: 0 608 756
Size: 608 148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 562 603 756
Size: 100 462 41 153
Fragments arranged by size:
462 153 100 41
AfeI AGC'GCT
Cuts at: 0 560 756
Size: 560 196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 530 562 756
Size: 530 32 194
Fragments arranged by size:
530 194 32
AlwNI CAG_nnn'CTG
Cuts at: 0 90 266 756
Size: 90 176 490
Fragments arranged by size:
490 176 90
BanII G_rGCy'C
Cuts at: 0 35 128 186 756
Size: 35 93 58 570
Fragments arranged by size:
570 93 58 35
BbvCI CC'TCA_GC
Cuts at: 0 279 756
Size: 279 477
BglI GCCn_nnn'nGGC
Cuts at: 0 151 220 581 756
Size: 151 69 361 175
Fragments arranged by size:
361 175 151 69
BlpI GC'TnA_GC
Cuts at: 0 122 442 628 756
Size: 122 320 186 128
Fragments arranged by size:
320 186 128 122
Bme1580I G_kGCm'C
Cuts at: 0 205 742 756
Size: 205 537 14
Fragments arranged by size:
537 205 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 529 756
Size: 529 227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 664 718 756
Size: 112 552 54 38
Fragments arranged by size:
552 112 54 38
Bpu10I CC'TnA_GC
Cuts at: 0 127 279 399 756
Size: 127 152 120 357
Fragments arranged by size:
357 152 127 120
BsaI GGTCTCn'nnnn_
Cuts at: 0 237 756
Size: 237 519
BsaAI yAC'GTr
Cuts at: 0 454 756
Size: 454 302
BseYI C'CCAG_C
Cuts at: 0 198 597 756
Size: 198 399 159
Fragments arranged by size:
399 198 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 756
Size: 661 95
BsiEI CG_ry'CG
Cuts at: 0 663 669 756
Size: 663 6 87
Fragments arranged by size:
663 87 6
BsiHKAI G_wGCw'C
Cuts at: 0 128 186 443 756
Size: 128 58 257 313
Fragments arranged by size:
313 257 128 58
Bsp1286I G_dGCh'C
Cuts at: 0 35 128 186 205 443 742 756
Size: 35 93 58 19 238 299 14
Fragments arranged by size:
299 238 93 58 35 19 14
BsrDI GCAATG_nn'
Cuts at: 0 423 756
Size: 423 333
BsrFI r'CCGG_y
Cuts at: 0 486 756
Size: 486 270
BssSI C'ACGA_G
Cuts at: 0 406 756
Size: 406 350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 226 458 642 756
Size: 226 232 184 114
Fragments arranged by size:
232 226 184 114
BtgI C'CryG_G
Cuts at: 0 225 663 756
Size: 225 438 93
Fragments arranged by size:
438 225 93
BtsI GCAGTG_nn'
Cuts at: 0 203 756
Size: 203 553
DraIII CAC_nnn'GTG
Cuts at: 0 684 746 756
Size: 684 62 10
Fragments arranged by size:
684 62 10
EaeI y'GGCC_r
Cuts at: 0 212 324 410 488 533 660 666 756
Size: 212 112 86 78 45 127 6 90
Fragments arranged by size:
212 127 112 90 86 78 45 6
EagI C'GGCC_G
Cuts at: 0 660 666 756
Size: 660 6 90
Fragments arranged by size:
660 90 6
EarI CTCTTCn'nnn_
Cuts at: 0 185 295 756
Size: 185 110 461
Fragments arranged by size:
461 185 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 365 756
Size: 365 391
EcoICRI GAG'CTC
Cuts at: 0 126 184 756
Size: 126 58 572
Fragments arranged by size:
572 126 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 112 562 603 664 718 756
Size: 100 12 450 41 61 54 38
Fragments arranged by size:
450 100 61 54 41 38 12
EcoO109I rG'GnC_Cy
Cuts at: 0 14 87 97 164 263 270 573 725
Size: 14 73 10 67 99 7 303 152
Cuts at: 725 756
Size: 31
Fragments arranged by size:
303 152 99 73 67 31 14 10
7
HaeII r_GCGC'y
Cuts at: 0 562 756
Size: 562 194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 262 294 756
Size: 17 32 213 32 462
Fragments arranged by size:
462 213 32 32 17
MscI TGG'CCA
Cuts at: 0 214 326 412 535 756
Size: 214 112 86 123 221
Fragments arranged by size:
221 214 123 112 86
MspA1I CmG'CkG
Cuts at: 0 480 665 756
Size: 480 185 91
Fragments arranged by size:
480 185 91
NcoI C'CATG_G
Cuts at: 0 225 756
Size: 225 531
NotI GC'GGCC_GC
Cuts at: 0 666 756
Size: 666 90
NspI r_CATG'y
Cuts at: 0 110 756
Size: 110 646
PasI CC'CwG_GG
Cuts at: 0 60 240 381 756
Size: 60 180 141 375
Fragments arranged by size:
375 180 141 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 54 90 756
Size: 54 36 666
Fragments arranged by size:
666 54 36
PmlI CAC'GTG
Cuts at: 0 454 756
Size: 454 302
PpuMI rG'GwC_Cy
Cuts at: 0 263 270 725 756
Size: 263 7 455 31
Fragments arranged by size:
455 263 31 7
PstI C_TGCA'G
Cuts at: 0 509 592 756
Size: 509 83 164
Fragments arranged by size:
509 164 83
PvuII CAG'CTG
Cuts at: 0 480 756
Size: 480 276
SacI G_AGCT'C
Cuts at: 0 128 186 756
Size: 128 58 570
Fragments arranged by size:
570 128 58
SacII CC_GC'GG
Cuts at: 0 666 756
Size: 666 90
SapI GCTCTTCn'nnn_
Cuts at: 0 185 295 756
Size: 185 110 461
Fragments arranged by size:
461 185 110
SfcI C'TryA_G
Cuts at: 0 505 588 756
Size: 505 83 168
Fragments arranged by size:
505 168 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 220 756
Size: 220 536
SphI G_CATG'C
Cuts at: 0 110 756
Size: 110 646
StuI AGG'CCT
Cuts at: 0 358 756
Size: 358 398
StyI C'CwwG_G
Cuts at: 0 225 756
Size: 225 531
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 232 756
Size: 232 524
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 515 547 756
Size: 515 32 209
Fragments arranged by size:
515 209 32
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI BanII BbvCI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BsrDI BsrFI BssSI BstXI
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I MscI MspA1I NcoI NotI
NspI PasI PflMI PmlI PpuMI PstI PvuII SacI
SacII SapI SfcI SfiI SphI StuI StyI TaqII
TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglII BmgBI BmtI BplI BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NdeI NgoMIV NheI NruI NsiI
PacI PciI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI