################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII AccII AclI AcsI AcyI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MfeI MluI MluNI MscI MslI MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 433 GGTACC G^GTACC AccI 286 GTCTAC GT^[AC][GT]AC AccIII 329 TCCGGA T^CCGGA AgeI 43 ACCGGT A^CCGGT Asp718I 433 GGTACC G^GTACC BamHI 167 GGATCC G^GATCC BanI 433 GGTACC G^G[CT][GA]CC BbsI 399 GAAGAC GAAGAC(2/6) BcnI 18 CCGGG CC^[GC]GG BfaI 206 CTAG C^TAG BpmI 95 CTCCAG CTGGAG(16/14) BpuAI 399 GAAGAC GAAGAC(2/6) BsaBI 127 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 227 GAGACC GGTCTC(1/5) BseAI 329 TCCGGA T^CCGGA BsmAI 227 GAGAC GTCTC(1/5) BsmFI 344 GGGAC GGGAC(10/14) BspEI 329 TCCGGA T^CCGGA BspMI 242 ACCTGC ACCTGC(4/8) BstXI 107 CCAGGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 147 GCGC GCG^C DdeI 382 CTGAG C^T[ACGT]AG HaeII 146 AGCGCC [GA]GCGC^[CT] HgaI 260 GACGC GACGC(5/10) HhaI 147 GCGC GCG^C HinP1I 147 GCGC G^CGC HinfI 118 GAGTC G^A[ACGT]TC KpnI 433 GGTACC GGTAC^C MaeI 206 CTAG C^TAG MaeII 368 ACGT A^CGT MamI 127 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 329 TCCGGA T^CCGGA MseI 470 TTAA T^TAA NciI 18 CCGGG CC^[GC]GG PinAI 43 ACCGGT A^CCGGT PleI 118 GAGTC GAGTC(4/5) PshAI 402 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 358 CTGCAG CTGCA^G SfaNI 270 GATGC GCATC(5/9) SfcI 358 CTGCAG C^T[GA][CT]AG Tru9I 470 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 256 GTAC GT^AC 434 GTAC AluI 6 AGCT AG^CT 467 AGCT ApaI 90 GGGCCC GGGCC^C 353 GGGCCC Asp700I 373 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 489 GAAAGGCTTC BsaWI 43 ACCGGT [AT]^CCGG[AT] 329 TCCGGA BstYI 167 GGATCC [GA]^GATC[CT] 174 AGATCC HaeIII 91 GGCC GG^CC 354 GGCC HphI 426 GGTGA GGTGA(8/7) 410 TCACC MboII 373 GAAGA GAAGA(8/7) 399 GAAGA MflI 167 GGATCC [GA]^GATC[CT] 174 AGATCC MspA1I 52 CAGCGG C[AC]G^C[GT]G 388 CCGCTG RsaI 256 GTAC GT^AC 434 GTAC Tsp45I 409 GTCAC ^GT[GC]AC 427 GTGAC XhoII 167 GGATCC [GA]^GATC[CT] 174 AGATCC XmnI 373 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 489 GAAAGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AlwI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HapII HpaII ItaI MaeIII MboI MnlI MspI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI