Restriction map for
pFN21AE5174
(Circular) (Six-base) MAPSORT of: pFN21AE5174.seq Check: 7006 from: 1 to: 5203
pFN21AE5174 5203 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3013 278
Size: 53 83 186 936 1477 2468
Fragments arranged by size:
2468 1477 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2292 2546 1937
Size: 355 254 4594
Fragments arranged by size:
4594 355 254
Acc65I G'GTAC_C
Cuts at: 2438 2524 2438
Size: 86 5117
AclI AA'CG_TT
Cuts at: 1563 3330 3703 1563
Size: 1767 373 3063
Fragments arranged by size:
3063 1767 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3266 4650 4918 1632
Size: 1634 1384 268 1917
Fragments arranged by size:
1917 1634 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5006
AflIII A'CryG_T
Cuts at: 1203 4102 5079 1203
Size: 2899 977 1327
Fragments arranged by size:
2899 1327 977
AgeI A'CCGG_T
Cuts at: 2048 4034 2048
Size: 1986 3217
AhdI GACnn_n'nnGTC
Cuts at: 3932 3932
Size: 5203
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5203
AlwNI CAG_nnn'CTG
Cuts at: 2946 4518 2946
Size: 1572 3631
ApaI G_GGCC'C
Cuts at: 2099 2362 2099
Size: 263 4940
ApaLI G'TGCA_C
Cuts at: 3260 4416 3260
Size: 1156 4047
ApoI r'AATT_y
Cuts at: 1493 2512 2738 2890 4999 1493
Size: 1019 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3757 160
Size: 872 2725 1606
Fragments arranged by size:
2725 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5203
AvaI C'yCGr_G
Cuts at: 1870 1943 2528 1870
Size: 73 585 4545
Fragments arranged by size:
4545 585 73
BamHI G'GATC_C
Cuts at: 2172 2533 2900 2172
Size: 361 367 4475
Fragments arranged by size:
4475 367 361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2438 2524 3979 618
Size: 325 217 627 651 86 1455 1842
Fragments arranged by size:
1842 1455 651 627 325 217 86
BanII G_rGCy'C
Cuts at: 1391 2099 2362 2393 2522 1391
Size: 708 263 31 129 4072
Fragments arranged by size:
4072 708 263 129 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2411 928
Size: 971 512 3720
Fragments arranged by size:
3720 971 512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3394 3428 1903
Size: 34 1457 34 3678
Fragments arranged by size:
3678 1457 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3095 4305 3095
Size: 1210 3993
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5203
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3814 136
Size: 107 122 71 631 605 2142 1525
Fragments arranged by size:
2142 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5198 5198
Size: 5203
BlpI GC'TnA_GC
Cuts at: 2620 2620
Size: 5203
Bme1580I G_kGCm'C
Cuts at: 1790 2099 2362 3264 4420 1790
Size: 309 263 902 1156 2573
Fragments arranged by size:
2573 1156 902 309 263
BmgBI CAC'GTC
Cuts at: 1318 2374 1318
Size: 1056 4147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2934 3892 442
Size: 416 53 83 399 24 1517 958 1753
Fragments arranged by size:
1753 1517 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2085 3863 1239
Size: 846 1778 2579
Fragments arranged by size:
2579 1778 846
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3328 4193 4491 3328
Size: 865 298 4040
Fragments arranged by size:
4040 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2226 3866 882
Size: 617 727 1640 2219
Fragments arranged by size:
2219 1640 727 617
BsaAI yAC'GTr
Cuts at: 493 1204 2994 493
Size: 711 1790 2702
Fragments arranged by size:
2702 1790 711
BsaBI GATnn'nnATC
Cuts at: 2136 5197 2136
Size: 3061 2142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3010 3392 4027 275
Size: 53 83 186 936 1477 382 635 1451
Fragments arranged by size:
1477 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2048 2334 3636 4034 4308 4455 4866 2048
Size: 286 1302 398 274 147 411 2385
Fragments arranged by size:
2385 1302 411 398 286 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2035 2065 2141 2171 1178
Size: 30 165 30 632 30 76 30 4210
Fragments arranged by size:
4210 632 165 76 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5203
BseYI C'CCAG_C
Cuts at: 2398 4406 4902 2398
Size: 2008 496 2699
Fragments arranged by size:
2699 2008 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4630
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2653 3414 4442 1540
Size: 275 186 652 761 1028 2301
Fragments arranged by size:
2301 1028 761 652 275 186
BsiHKAI G_wGCw'C
Cuts at: 3264 3349 4420 3264
Size: 85 1071 4047
Fragments arranged by size:
4047 1071 85
BsmI GAATG_Cn'
Cuts at: 2714 2807 2714
Size: 93 5110
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2362 2393 2522 3264 3349 4420
Size: 399 309 263 31 129 742 85 1071
Cuts at: 4420 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 309 263 129 85
31
BspEI T'CCGG_A
Cuts at: 2334 4866 2334
Size: 2532 2671
BspHI T'CATG_A
Cuts at: 3092 4822 3092
Size: 1730 3473
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2256 2558 844
Size: 1412 302 3489
Fragments arranged by size:
3489 1412 302
BsrBI CCG'CTC
Cuts at: 1394 1823 3090 1394
Size: 429 1267 3507
Fragments arranged by size:
3507 1267 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3698 3872 65
Size: 1183 2450 174 1396
Fragments arranged by size:
2450 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2161 2316 3847 4034 1700
Size: 110 238 113 155 1531 187 2869
Fragments arranged by size:
2869 1531 238 187 155 113 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5203
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5203
BssSI C'ACGA_G
Cuts at: 3263 4275 3263
Size: 1012 4191
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5203
BstBI TT'CG_AA
Cuts at: 4007 4007
Size: 5203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5203
BstXI CCAn_nnnn'nTGG
Cuts at: 2119 2119
Size: 5203
BstYI r'GATC_y
Cuts at: 1972 2172 2179 2533 2900 3285 3302 4754 4840
Size: 200 7 354 367 385 17 1452 86
Cuts at: 4840 4852 5198 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 354 346 200 86
17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3712
Fragments arranged by size:
3712 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4464
Fragments arranged by size:
4464 683 56
BtsI GCAGTG_nn'
Cuts at: 2719 3513 3533 2719
Size: 794 20 4389
Fragments arranged by size:
4389 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5168
DraI TTT'AAA
Cuts at: 2507 2507
Size: 5203
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4210 809
Size: 507 2894 1802
Fragments arranged by size:
2894 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2650 3539 5168
Size: 54 1425 50 275 18 820 889 1629
Cuts at: 5168 8
Size: 43
Fragments arranged by size:
1629 1425 889 820 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2650 1537
Size: 275 838 4090
Fragments arranged by size:
4090 838 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3133 1353
Size: 1780 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3790 4164 4310 387
Size: 1064 138 2201 374 146 1280
Fragments arranged by size:
2201 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 3266 3863 4650 4918 1239
Size: 393 453 1181 597 787 268 1524
Fragments arranged by size:
1524 1181 787 597 453 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2095 2096 2359 2095
Size: 1 263 4939
Fragments arranged by size:
4939 263 1
EcoRI G'AATT_C
Cuts at: 2512 2890 2512
Size: 378 4825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 5203
FspI TGC'GCA
Cuts at: 3709 3709
Size: 5203
HaeII r_GCGC'y
Cuts at: 1678 2155 4350 1678
Size: 477 2195 2531
Fragments arranged by size:
2531 2195 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 3844 3876 3918
Size: 32 135 32 592 32 1648 32 42
Cuts at: 3918 3950 1373
Size: 32 2626
Fragments arranged by size:
2626 1648 592 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2547 2793 669
Size: 988 281 609 246 3079
Fragments arranged by size:
3079 988 609 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5203
HpaI GTT'AAC
Cuts at: 2793 2793
Size: 5203
KpnI G_GTAC'C
Cuts at: 2442 2528 2442
Size: 86 5117
MfeI C'AATT_G
Cuts at: 2802 2802
Size: 5203
MluI A'CGCG_T
Cuts at: 5079 5079
Size: 5203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4317 4501 1305
Size: 3012 184 2007
Fragments arranged by size:
3012 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3381
Fragments arranged by size:
3381 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3161 3520 3679 518
Size: 648 1995 359 159 2042
Fragments arranged by size:
2042 1995 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2395 2619 3296 4444 4689 1606
Size: 453 336 224 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 453 336 245 224
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3712
Fragments arranged by size:
3712 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5203
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5203
NotI GC'GGCC_GC
Cuts at: 2650 2650
Size: 5203
NspI r_CATG'y
Cuts at: 1132 1229 2561 4106 1132
Size: 97 1332 1545 2229
Fragments arranged by size:
2229 1545 1332 97
PasI CC'CwG_GG
Cuts at: 1348 2092 1348
Size: 744 4459
PciI A'CATG_T
Cuts at: 4102 4102
Size: 5203
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5203
PmeI GTTT'AAAC
Cuts at: 2507 2507
Size: 5203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 4785 4817 3272
Size: 32 1481 32 3658
Fragments arranged by size:
3658 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2411 1613
Size: 798 4405
PsiI TTA'TAA
Cuts at: 2773 2773
Size: 5203
PspOMI G'GGCC_C
Cuts at: 2095 2358 2095
Size: 263 4940
PstI C_TGCA'G
Cuts at: 830 2367 2555 830
Size: 1537 188 3478
Fragments arranged by size:
3478 1537 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5203
SalI G'TCGA_C
Cuts at: 1936 2545 1936
Size: 609 4594
SbfI CC_TGCA'GG
Cuts at: 2555 2555
Size: 5203
ScaI AGT'ACT
Cuts at: 3451 5163 3451
Size: 1712 3491
SfcI C'TryA_G
Cuts at: 826 1045 2363 2551 3686 4367 4558 826
Size: 219 1318 188 1135 681 191 1471
Fragments arranged by size:
1471 1318 1135 681 219 191 188
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5203
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5203
SmaI CCC'GGG
Cuts at: 1872 2530 1872
Size: 658 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3307 4208 4470 820
Size: 197 926 1364 901 262 1553
Fragments arranged by size:
1553 1364 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2994 493
Size: 2501 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5203
SphI G_CATG'C
Cuts at: 1132 2561 1132
Size: 1429 3774
SspI AAT'ATT
Cuts at: 5 52 3127 5
Size: 47 3075 2081
Fragments arranged by size:
3075 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5203
StyI C'CwwG_G
Cuts at: 513 1065 2004 2642 513
Size: 552 939 638 3074
Fragments arranged by size:
3074 939 638 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2257 2432 3230 3247 3400 3585 4030
Size: 1255 677 175 798 17 153 185 445
Cuts at: 4030 325
Size: 1498
Fragments arranged by size:
1498 1255 798 677 445 185 175 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3449 5161 96
Size: 274 80 33 51 1443 1472 1712 138
Fragments arranged by size:
1712 1472 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2035 2067 5163 5195 2035
Size: 32 3096 32 2043
Fragments arranged by size:
3096 2043 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5203
XbaI T'CTAG_A
Cuts at: 2539 2539
Size: 5203
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5203
XmaI C'CCGG_G
Cuts at: 1870 2528 1870
Size: 658 4545
XmnI GAAnn'nnTTC
Cuts at: 2382 2498 3332 2382
Size: 116 834 4253
Fragments arranged by size:
4253 834 116
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3011 276
Size: 53 83 186 936 1477 2468
Fragments arranged by size:
2468 1477 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI