(Linear) (Six-base) MAPSORT of: KIEE5174.seq Check: 3859 from: 1 to: 498 KIEE5174.orf With 182 enzymes: SgfI * January 29, 2014 11:49 .. AccI GT'mk_AC Cuts at: 0 287 498 Size: 287 211 Acc65I G'GTAC_C Cuts at: 0 433 498 Size: 433 65 AgeI A'CCGG_T Cuts at: 0 43 498 Size: 43 455 ApaI G_GGCC'C Cuts at: 0 94 357 498 Size: 94 263 141 Fragments arranged by size: 263 141 94 BamHI G'GATC_C Cuts at: 0 167 498 Size: 167 331 BanI G'GyrC_C Cuts at: 0 433 498 Size: 433 65 BanII G_rGCy'C Cuts at: 0 94 357 388 498 Size: 94 263 31 110 Fragments arranged by size: 263 110 94 31 BbsI GAAGACnn'nnnn_ Cuts at: 0 406 498 Size: 406 92 Bme1580I G_kGCm'C Cuts at: 0 94 357 498 Size: 94 263 141 Fragments arranged by size: 263 141 94 BmgBI CAC'GTC Cuts at: 0 369 498 Size: 369 129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 498 Size: 80 418 BsaI GGTCTCn'nnnn_ Cuts at: 0 221 498 Size: 221 277 BsaBI GATnn'nnATC Cuts at: 0 131 498 Size: 131 367 BsaWI w'CCGG_w Cuts at: 0 43 329 498 Size: 43 286 169 Fragments arranged by size: 286 169 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 30 60 136 166 498 Size: 30 30 76 30 332 Fragments arranged by size: 332 76 30 30 30 BseYI C'CCAG_C Cuts at: 0 393 498 Size: 393 105 Bsp1286I G_dGCh'C Cuts at: 0 94 357 388 498 Size: 94 263 31 110 Fragments arranged by size: 263 110 94 31 BspEI T'CCGG_A Cuts at: 0 329 498 Size: 329 169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 251 498 Size: 251 247 BsrFI r'CCGG_y Cuts at: 0 43 156 311 498 Size: 43 113 155 187 Fragments arranged by size: 187 155 113 43 BstXI CCAn_nnnn'nTGG Cuts at: 0 114 498 Size: 114 384 BstYI r'GATC_y Cuts at: 0 167 174 498 Size: 167 7 324 Fragments arranged by size: 324 167 7 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 80 498 Size: 80 418 EcoO109I rG'GnC_Cy Cuts at: 0 90 91 354 498 Size: 90 1 263 144 Fragments arranged by size: 263 144 90 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 471 498 Size: 471 27 HaeII r_GCGC'y Cuts at: 0 150 498 Size: 150 348 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 159 191 498 Size: 159 32 307 Fragments arranged by size: 307 159 32 KpnI G_GTAC'C Cuts at: 0 437 498 Size: 437 61 MspA1I CmG'CkG Cuts at: 0 54 390 498 Size: 54 336 108 Fragments arranged by size: 336 108 54 PasI CC'CwG_GG Cuts at: 0 87 498 Size: 87 411 PshAI GACnn'nnGTC Cuts at: 0 406 498 Size: 406 92 PspOMI G'GGCC_C Cuts at: 0 90 353 498 Size: 90 263 145 Fragments arranged by size: 263 145 90 PstI C_TGCA'G Cuts at: 0 362 498 Size: 362 136 SfcI C'TryA_G Cuts at: 0 358 498 Size: 358 140 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 252 427 498 Size: 252 175 71 Fragments arranged by size: 252 175 71 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 30 62 498 Size: 30 32 436 Fragments arranged by size: 436 32 30 XmnI GAAnn'nnTTC Cuts at: 0 377 493 498 Size: 377 116 5 Fragments arranged by size: 377 116 5 Enzymes that do cut: AccI Acc65I AgeI ApaI BamHI BanI BanII BbsI Bme1580I BmgBI BpmI BsaI BsaBI BsaWI BsaXI BseYI Bsp1286I BspEI BspMI BsrFI BstXI BstYI Eco57MI EcoO109I FalI HaeII Hin4I KpnI MspA1I PasI PshAI PspOMI PstI SfcI TaqII TstI XmnI Enzymes that do not cut: AarI AatII AclI AcuI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI