(Circular) (Six-base) MAPSORT of: pFN21AE6483.seq Check: 394 from: 1 to: 6364 pFN21AE6483 6364 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3362 3362 Size: 6364 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4174 278 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3707 1937 Size: 1770 4594 Acc65I G'GTAC_C Cuts at: 3685 3685 Size: 6364 AclI AA'CG_TT Cuts at: 1563 4491 4864 1563 Size: 2928 373 3063 Fragments arranged by size: 3063 2928 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 2531 2710 4427 5811 6079 1632 Size: 824 75 179 1717 1384 268 1917 Fragments arranged by size: 1917 1717 1384 824 268 179 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6167 AflIII A'CryG_T Cuts at: 1203 5263 6240 1203 Size: 4060 977 1327 Fragments arranged by size: 4060 1327 977 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 5093 5093 Size: 6364 AleI CACnn'nnGTG Cuts at: 1166 2396 3278 1166 Size: 1230 882 4252 Fragments arranged by size: 4252 1230 882 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2543 1184 Size: 1359 5005 AlwNI CAG_nnn'CTG Cuts at: 2899 3124 4107 5679 2899 Size: 225 983 1572 3584 Fragments arranged by size: 3584 1572 983 225 ApaI G_GGCC'C Cuts at: 2246 2692 2246 Size: 446 5918 ApaLI G'TGCA_C Cuts at: 4421 5577 4421 Size: 1156 5208 ApoI r'AATT_y Cuts at: 1493 3673 3899 4051 6160 1493 Size: 2180 226 152 2109 1697 Fragments arranged by size: 2180 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 3551 3689 1870 Size: 73 1608 138 4545 Fragments arranged by size: 4545 1608 138 73 AvrII C'CTAG_G Cuts at: 2194 2194 Size: 6364 BamHI G'GATC_C Cuts at: 3694 4061 3694 Size: 367 5997 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2155 3017 3188 3280 3514 Size: 325 217 627 368 862 171 92 234 Cuts at: 3514 3685 5140 618 Size: 171 1455 1842 Fragments arranged by size: 1842 1455 862 627 368 325 234 217 171 171 92 BanII G_rGCy'C Cuts at: 1391 2246 2692 3258 3301 3381 3683 1391 Size: 855 446 566 43 80 302 4072 Fragments arranged by size: 4072 855 566 446 302 80 43 BbeI G_GCGC'C Cuts at: 3192 3192 Size: 6364 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5393 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4555 4589 1903 Size: 34 2618 34 3678 Fragments arranged by size: 3678 2618 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4256 5466 4256 Size: 1210 5154 BclI T'GATC_A Cuts at: 1548 1548 Size: 6364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2222 2290 3213 Size: 107 122 71 631 605 550 68 923 Cuts at: 3213 4975 136 Size: 1762 1525 Fragments arranged by size: 1762 1525 923 631 605 550 122 107 71 68 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 2987 3203 3307 3781 2987 Size: 216 104 474 5570 Fragments arranged by size: 5570 474 216 104 Bme1580I G_kGCm'C Cuts at: 1790 2139 2158 2246 2660 2692 3020 3285 4425 Size: 349 19 88 414 32 328 265 1140 Cuts at: 4425 5581 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1140 414 349 328 265 88 32 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6364 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2443 4095 5053 Size: 416 53 83 399 24 1026 1652 958 Cuts at: 5053 442 Size: 1753 Fragments arranged by size: 1753 1652 1026 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2140 2140 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2135 2347 2557 5024 1239 Size: 806 90 212 210 2467 2579 Fragments arranged by size: 2579 2467 806 212 210 90 Bpu10I CC'TnA_GC Cuts at: 2181 3395 2181 Size: 1214 5150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2224 4489 5354 5652 2224 Size: 2265 865 298 2936 Fragments arranged by size: 2936 2265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2086 2803 3548 5027 882 Size: 617 587 717 745 1479 2219 Fragments arranged by size: 2219 1479 745 717 617 587 BsaAI yAC'GTr Cuts at: 493 1204 4155 493 Size: 711 2951 2702 Fragments arranged by size: 2951 2702 711 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2640 3189 4171 4553 Size: 53 83 186 936 1107 549 982 382 Cuts at: 4553 5188 275 Size: 635 1451 Fragments arranged by size: 1451 1107 982 936 635 549 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3048 4797 5195 5469 5616 6027 3048 Size: 1749 398 274 147 411 3385 Fragments arranged by size: 3385 1749 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2191 2221 2844 2874 1178 Size: 30 165 30 788 30 623 30 4668 Fragments arranged by size: 4668 788 623 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2852 2996 3453 1186 Size: 1666 144 457 4097 Fragments arranged by size: 4097 1666 457 144 BseYI C'CCAG_C Cuts at: 2138 2151 2361 2465 3175 3322 3328 3469 3631 Size: 13 210 104 710 147 6 141 162 Cuts at: 3631 5567 6063 2138 Size: 1936 496 2439 Fragments arranged by size: 2439 1936 710 496 210 162 147 141 104 13 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2957 3614 1163 Size: 573 1221 657 3913 Fragments arranged by size: 3913 1221 657 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2130 3814 4575 5603 1540 Size: 275 186 129 1684 761 1028 2301 Fragments arranged by size: 2301 1684 1028 761 275 186 129 BsiHKAI G_wGCw'C Cuts at: 3381 3396 4425 4510 5581 3381 Size: 15 1029 85 1071 4164 Fragments arranged by size: 4164 1071 1029 85 15 BsmI GAATG_Cn' Cuts at: 3261 3875 3968 3261 Size: 614 93 5657 Fragments arranged by size: 5657 614 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2158 2246 2660 2692 3020 3258 Size: 399 349 19 88 414 32 328 238 Cuts at: 3258 3285 3301 3381 3396 3683 4425 4510 5581 Size: 27 16 80 15 287 742 85 1071 Cuts at: 5581 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 414 399 349 328 287 238 88 85 80 32 27 19 16 15 BspEI T'CCGG_A Cuts at: 3048 6027 3048 Size: 2979 3385 BspHI T'CATG_A Cuts at: 2643 4253 5983 2643 Size: 1610 1730 3024 Fragments arranged by size: 3024 1730 1610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2037 3123 3362 3719 844 Size: 1193 1086 239 357 3489 Fragments arranged by size: 3489 1193 1086 357 239 BsrBI CCG'CTC Cuts at: 1394 1823 4251 1394 Size: 429 2428 3507 Fragments arranged by size: 3507 2428 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4859 5033 65 Size: 1183 3611 174 1396 Fragments arranged by size: 3611 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5008 5195 1700 Size: 110 3198 187 2869 Fragments arranged by size: 3198 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6364 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6364 BssSI C'ACGA_G Cuts at: 2030 2414 4424 5436 2030 Size: 384 2010 1012 2958 Fragments arranged by size: 2958 2010 1012 384 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6364 BstBI TT'CG_AA Cuts at: 5168 5168 Size: 6364 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6364 BstXI CCAn_nnnn'nTGG Cuts at: 2398 2461 3277 2398 Size: 63 816 5485 Fragments arranged by size: 5485 816 63 BstYI r'GATC_y Cuts at: 1972 3694 4061 4446 4463 5915 6001 6013 6359 Size: 1722 367 385 17 1452 86 12 346 Cuts at: 6359 1972 Size: 1977 Fragments arranged by size: 1977 1722 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2211 2955 2211 Size: 744 5620 BtgI C'CryG_G Cuts at: 513 1065 2004 2313 2442 2631 2737 3433 3478 Size: 552 939 309 129 189 106 696 45 Cuts at: 3478 513 Size: 3399 Fragments arranged by size: 3399 939 696 552 309 189 129 106 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5625 Fragments arranged by size: 5625 683 56 BtsI GCAGTG_nn' Cuts at: 2760 2780 2940 3414 3539 3880 4674 4694 2760 Size: 20 160 474 125 341 794 20 4430 Fragments arranged by size: 4430 794 474 341 160 125 20 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3643 3678 529 Size: 35 3079 35 3215 Fragments arranged by size: 3215 3079 35 35 DraI TTT'AAA Cuts at: 3668 3668 Size: 6364 DraIII CAC_nnn'GTG Cuts at: 3069 3069 Size: 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5371 809 Size: 507 4055 1802 Fragments arranged by size: 4055 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2316 2400 Size: 54 1425 50 275 18 297 189 84 Cuts at: 2400 2734 3231 3267 3315 3534 3811 4700 6329 Size: 334 497 36 48 219 277 889 1629 Cuts at: 6329 8 Size: 43 Fragments arranged by size: 1629 1425 889 497 334 297 277 275 219 189 84 54 50 48 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2127 3811 1537 Size: 275 315 1684 4090 Fragments arranged by size: 4090 1684 315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3386 4294 1353 Size: 2033 908 3423 Fragments arranged by size: 3423 2033 908 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2267 4951 5325 5471 387 Size: 1064 138 678 2684 374 146 1280 Fragments arranged by size: 2684 1280 1064 678 374 146 138 EcoICRI GAG'CTC Cuts at: 3379 3379 Size: 6364 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2135 2347 2456 2531 2557 2710 Size: 393 413 90 212 109 75 26 153 Cuts at: 2710 4427 5024 5811 6079 1239 Size: 1717 597 787 268 1524 Fragments arranged by size: 1717 1524 787 597 413 393 268 212 153 109 90 75 26 EcoNI CCTnn'n_nnAGG Cuts at: 2506 2506 Size: 6364 EcoO109I rG'GnC_Cy Cuts at: 2215 2243 2256 2887 3121 3452 2215 Size: 28 13 631 234 331 5127 Fragments arranged by size: 5127 631 331 234 28 13 EcoRI G'AATT_C Cuts at: 3673 4051 3673 Size: 378 5986 FspI TGC'GCA Cuts at: 4870 4870 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 3192 5511 1678 Size: 1514 2319 2531 Fragments arranged by size: 2531 2319 1514 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2056 2088 2191 2223 2245 Size: 32 135 32 484 32 103 32 22 Cuts at: 2245 2277 2847 2879 3429 3461 5005 5037 5079 Size: 32 570 32 550 32 1544 32 42 Cuts at: 5079 5111 1373 Size: 32 2626 Fragments arranged by size: 2626 1544 570 550 484 135 103 42 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3708 3954 669 Size: 988 281 1770 246 3079 Fragments arranged by size: 3079 1770 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KasI G'GCGC_C Cuts at: 3188 3188 Size: 6364 KpnI G_GTAC'C Cuts at: 3689 3689 Size: 6364 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 6240 6240 Size: 6364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5478 5662 1305 Size: 4173 184 2007 Fragments arranged by size: 4173 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2402 3269 10 Size: 54 1425 343 570 867 3105 Fragments arranged by size: 3105 1425 867 570 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 3278 4322 4681 4840 518 Size: 648 1230 882 1044 359 159 2042 Fragments arranged by size: 2042 1230 1044 882 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2148 2325 2573 2750 2791 3571 3780 4457 Size: 542 177 248 177 41 780 209 677 Cuts at: 4457 5605 5850 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 780 677 542 248 245 209 177 177 41 NarI GG'CG_CC Cuts at: 3189 3189 Size: 6364 NcoI C'CATG_G Cuts at: 513 1065 2004 2442 2737 3478 513 Size: 552 939 438 295 741 3399 Fragments arranged by size: 3399 939 741 552 438 295 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 3811 3811 Size: 6364 NspI r_CATG'y Cuts at: 1132 1229 2113 3034 3722 5267 1132 Size: 97 884 921 688 1545 2229 Fragments arranged by size: 2229 1545 921 884 688 97 PasI CC'CwG_GG Cuts at: 1348 3124 1348 Size: 1776 4588 PciI A'CATG_T Cuts at: 5263 5263 Size: 6364 PflMI CCAn_nnn'nTGG Cuts at: 1108 2068 2091 2325 3292 1108 Size: 960 23 234 967 4180 Fragments arranged by size: 4180 967 960 234 23 PfoI T'CCnGG_A Cuts at: 2022 2163 2022 Size: 141 6223 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6364 PsiI TTA'TAA Cuts at: 3934 3934 Size: 6364 PspOMI G'GGCC_C Cuts at: 2242 2688 2242 Size: 446 5918 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2591 2623 2591 Size: 32 6332 PstI C_TGCA'G Cuts at: 830 2755 2790 2895 3120 3716 830 Size: 1925 35 105 225 596 3478 Fragments arranged by size: 3478 1925 596 225 105 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 PvuII CAG'CTG Cuts at: 2325 2573 2750 2791 2325 Size: 248 177 41 5898 Fragments arranged by size: 5898 248 177 41 SacI G_AGCT'C Cuts at: 3381 3381 Size: 6364 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SapI GCTCTTCn'nnn_ Cuts at: 3386 3386 Size: 6364 SbfI CC_TGCA'GG Cuts at: 2895 3120 3716 2895 Size: 225 596 5543 Fragments arranged by size: 5543 596 225 ScaI AGT'ACT Cuts at: 4612 6324 4612 Size: 1712 4652 SfcI C'TryA_G Cuts at: 826 1045 2751 2786 2891 3116 3367 3712 4847 Size: 219 1706 35 105 225 251 345 1135 Cuts at: 4847 5528 5719 826 Size: 681 191 1471 Fragments arranged by size: 1706 1471 1135 681 345 251 225 219 191 105 35 SfoI GGC'GCC Cuts at: 3190 3190 Size: 6364 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 3691 1872 Size: 1819 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2203 4468 5369 5631 820 Size: 197 926 260 2265 901 262 1553 Fragments arranged by size: 2265 1553 926 901 262 260 197 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 2113 3034 3722 1132 Size: 981 921 688 3774 Fragments arranged by size: 3774 981 921 688 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6364 StyI C'CwwG_G Cuts at: 513 1065 2004 2194 2442 2697 2737 3312 3478 Size: 552 939 190 248 255 40 575 166 Cuts at: 3478 3803 513 Size: 325 3074 Fragments arranged by size: 3074 939 575 552 325 255 248 190 166 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2791 3461 4391 4408 4561 4746 Size: 1255 444 767 670 930 17 153 185 Cuts at: 4746 5191 325 Size: 445 1498 Fragments arranged by size: 1498 1255 930 767 670 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2598 2826 4610 Size: 274 80 33 51 1443 621 228 1784 Cuts at: 4610 6322 96 Size: 1712 138 Fragments arranged by size: 1784 1712 1443 621 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2876 6324 6356 2844 Size: 32 3448 32 2852 Fragments arranged by size: 3448 2852 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6364 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2875 3155 2875 Size: 280 6084 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6364 XmaI C'CCGG_G Cuts at: 1870 3689 1870 Size: 1819 4545 XmnI GAAnn'nnTTC Cuts at: 4493 4493 Size: 6364 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4172 276 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI