Restriction map for
pFN21AE6483
(Linear) (Six-base) MAPSORT of: KIEE6483.seq Check: 1171 from: 1 to: 1659
KIEE6483.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1357 1659
Size: 1357 302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 526 705 1659
Size: 451 75 179 954
Fragments arranged by size:
954 451 179 75
AleI CACnn'nnGTG
Cuts at: 0 391 1273 1659
Size: 391 882 386
Fragments arranged by size:
882 391 386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 538 1659
Size: 538 1121
AlwNI CAG_nnn'CTG
Cuts at: 0 894 1119 1659
Size: 894 225 540
Fragments arranged by size:
894 540 225
ApaI G_GGCC'C
Cuts at: 0 241 687 1659
Size: 241 446 972
Fragments arranged by size:
972 446 241
AvaI C'yCGr_G
Cuts at: 0 1546 1659
Size: 1546 113
AvrII C'CTAG_G
Cuts at: 0 189 1659
Size: 189 1470
BanI G'GyrC_C
Cuts at: 0 150 1012 1183 1275 1509 1659
Size: 150 862 171 92 234 150
Fragments arranged by size:
862 234 171 150 150 92
BanII G_rGCy'C
Cuts at: 0 241 687 1253 1296 1376 1659
Size: 241 446 566 43 80 283
Fragments arranged by size:
566 446 283 241 80 43
BbeI G_GCGC'C
Cuts at: 0 1187 1659
Size: 1187 472
BglI GCCn_nnn'nGGC
Cuts at: 0 217 285 1208 1659
Size: 217 68 923 451
Fragments arranged by size:
923 451 217 68
BlpI GC'TnA_GC
Cuts at: 0 982 1198 1302 1659
Size: 982 216 104 357
Fragments arranged by size:
982 357 216 104
Bme1580I G_kGCm'C
Cuts at: 0 134 153 241 655 687 1015 1280 1659
Size: 134 19 88 414 32 328 265 379
Fragments arranged by size:
414 379 328 265 134 88 32 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 438 1659
Size: 438 1221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 135 1659
Size: 135 1524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 130 342 552 1659
Size: 40 90 212 210 1107
Fragments arranged by size:
1107 212 210 90 40
Bpu10I CC'TnA_GC
Cuts at: 0 176 1390 1659
Size: 176 1214 269
Fragments arranged by size:
1214 269 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 1659
Size: 219 1440
BsaI GGTCTCn'nnnn_
Cuts at: 0 81 798 1543 1659
Size: 81 717 745 116
Fragments arranged by size:
745 717 116 81
BsaHI Gr'CG_yC
Cuts at: 0 635 1184 1659
Size: 635 549 475
Fragments arranged by size:
635 549 475
BsaWI w'CCGG_w
Cuts at: 0 1043 1659
Size: 1043 616
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 186 216 839 869 1659
Size: 186 30 623 30 790
Fragments arranged by size:
790 623 186 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 847 991 1448 1659
Size: 847 144 457 211
Fragments arranged by size:
847 457 211 144
BseYI C'CCAG_C
Cuts at: 0 133 146 356 460 1170 1317 1323 1464
Size: 133 13 210 104 710 147 6 141
Cuts at: 1464 1626 1659
Size: 162 33
Fragments arranged by size:
710 210 162 147 141 133 104 33
13 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1609 1659
Size: 952 657 50
Fragments arranged by size:
952 657 50
BsiEI CG_ry'CG
Cuts at: 0 125 1659
Size: 125 1534
BsiHKAI G_wGCw'C
Cuts at: 0 1376 1391 1659
Size: 1376 15 268
Fragments arranged by size:
1376 268 15
BsmI GAATG_Cn'
Cuts at: 0 1256 1659
Size: 1256 403
Bsp1286I G_dGCh'C
Cuts at: 0 134 153 241 655 687 1015 1253 1280
Size: 134 19 88 414 32 328 238 27
Cuts at: 1280 1296 1376 1391 1659
Size: 16 80 15 268
Fragments arranged by size:
414 328 268 238 134 88 80 32
27 19 16 15
BspEI T'CCGG_A
Cuts at: 0 1043 1659
Size: 1043 616
BspHI T'CATG_A
Cuts at: 0 638 1659
Size: 638 1021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 1118 1357 1659
Size: 32 1086 239 302
Fragments arranged by size:
1086 302 239 32
BssSI C'ACGA_G
Cuts at: 0 25 409 1659
Size: 25 384 1250
Fragments arranged by size:
1250 384 25
BstXI CCAn_nnnn'nTGG
Cuts at: 0 393 456 1272 1659
Size: 393 63 816 387
Fragments arranged by size:
816 393 387 63
Bsu36I CC'TnA_GG
Cuts at: 0 206 950 1659
Size: 206 744 709
Fragments arranged by size:
744 709 206
BtgI C'CryG_G
Cuts at: 0 308 437 626 732 1428 1473 1659
Size: 308 129 189 106 696 45 186
Fragments arranged by size:
696 308 189 186 129 106 45
BtsI GCAGTG_nn'
Cuts at: 0 755 775 935 1409 1534 1659
Size: 755 20 160 474 125 125
Fragments arranged by size:
755 474 160 125 125 20
DraIII CAC_nnn'GTG
Cuts at: 0 1064 1659
Size: 1064 595
EaeI y'GGCC_r
Cuts at: 0 122 311 395 729 1226 1262 1310 1529
Size: 122 189 84 334 497 36 48 219
Cuts at: 1529 1659
Size: 130
Fragments arranged by size:
497 334 219 189 130 122 84 48
36
EagI C'GGCC_G
Cuts at: 0 122 1659
Size: 122 1537
EarI CTCTTCn'nnn_
Cuts at: 0 1381 1659
Size: 1381 278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 262 1659
Size: 262 1397
EcoICRI GAG'CTC
Cuts at: 0 1374 1659
Size: 1374 285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 130 342 451 526 552 705 1659
Size: 40 90 212 109 75 26 153 954
Fragments arranged by size:
954 212 153 109 90 75 40 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 501 1659
Size: 501 1158
EcoO109I rG'GnC_Cy
Cuts at: 0 210 238 251 882 1116 1447 1659
Size: 210 28 13 631 234 331 212
Fragments arranged by size:
631 331 234 212 210 28 13
HaeII r_GCGC'y
Cuts at: 0 1187 1659
Size: 1187 472
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 51 83 186 218 240 272 842 874
Size: 51 32 103 32 22 32 570 32
Cuts at: 874 1424 1456 1659
Size: 550 32 203
Fragments arranged by size:
570 550 203 103 51 32 32 32
32 32 22
KasI G'GCGC_C
Cuts at: 0 1183 1659
Size: 1183 476
MscI TGG'CCA
Cuts at: 0 397 1264 1659
Size: 397 867 395
Fragments arranged by size:
867 397 395
MslI CAynn'nnrTG
Cuts at: 0 391 1273 1659
Size: 391 882 386
Fragments arranged by size:
882 391 386
MspA1I CmG'CkG
Cuts at: 0 143 320 568 745 786 1566 1659
Size: 143 177 248 177 41 780 93
Fragments arranged by size:
780 248 177 177 143 93 41
NarI GG'CG_CC
Cuts at: 0 1184 1659
Size: 1184 475
NcoI C'CATG_G
Cuts at: 0 437 732 1473 1659
Size: 437 295 741 186
Fragments arranged by size:
741 437 295 186
NspI r_CATG'y
Cuts at: 0 108 1029 1659
Size: 108 921 630
Fragments arranged by size:
921 630 108
PasI CC'CwG_GG
Cuts at: 0 1119 1659
Size: 1119 540
PflMI CCAn_nnn'nTGG
Cuts at: 0 63 86 320 1287 1659
Size: 63 23 234 967 372
Fragments arranged by size:
967 372 234 63 23
PfoI T'CCnGG_A
Cuts at: 0 17 158 1659
Size: 17 141 1501
Fragments arranged by size:
1501 141 17
PspOMI G'GGCC_C
Cuts at: 0 237 683 1659
Size: 237 446 976
Fragments arranged by size:
976 446 237
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 586 618 1659
Size: 586 32 1041
Fragments arranged by size:
1041 586 32
PstI C_TGCA'G
Cuts at: 0 750 785 890 1115 1659
Size: 750 35 105 225 544
Fragments arranged by size:
750 544 225 105 35
PvuII CAG'CTG
Cuts at: 0 320 568 745 786 1659
Size: 320 248 177 41 873
Fragments arranged by size:
873 320 248 177 41
SacI G_AGCT'C
Cuts at: 0 1376 1659
Size: 1376 283
SapI GCTCTTCn'nnn_
Cuts at: 0 1381 1659
Size: 1381 278
SbfI CC_TGCA'GG
Cuts at: 0 890 1115 1659
Size: 890 225 544
Fragments arranged by size:
890 544 225
SfcI C'TryA_G
Cuts at: 0 746 781 886 1111 1362 1659
Size: 746 35 105 225 251 297
Fragments arranged by size:
746 297 251 225 105 35
SfoI GGC'GCC
Cuts at: 0 1185 1659
Size: 1185 474
SmlI C'TyrA_G
Cuts at: 0 198 1659
Size: 198 1461
SphI G_CATG'C
Cuts at: 0 108 1029 1659
Size: 108 921 630
Fragments arranged by size:
921 630 108
StyI C'CwwG_G
Cuts at: 0 189 437 692 732 1307 1473 1659
Size: 189 248 255 40 575 166 186
Fragments arranged by size:
575 255 248 189 186 166 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 786 1456 1659
Size: 19 767 670 203
Fragments arranged by size:
767 670 203 19
TatI w'GTAC_w
Cuts at: 0 593 821 1659
Size: 593 228 838
Fragments arranged by size:
838 593 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 871 1659
Size: 839 32 788
Fragments arranged by size:
839 788 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 870 1150 1659
Size: 870 280 509
Fragments arranged by size:
870 509 280
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI AvaI AvrII
BanI BanII BbeI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BssSI BstXI Bsu36I BtgI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PfoI PspOMI PsrI PstI PvuII SacI
SapI SbfI SfcI SfoI SmlI SphI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BsaAI BsaBI BsiWI BsmBI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I
BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PvuI RsrII SacII SalI SanDI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI