################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MamI MluI MluNI MroI MscI MseI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 568 GTCTAC GT^[AC][GT]AC AccII 66 CGCG CG^CG AflIII 20 ACGTGT A^C[GA][CT]GT AlwNI 292 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 685 CCTCAGG CC^T[ACGT]AGG ApaI 681 GGGCCC GGGCC^C BamHI 867 GGATCC G^GATCC BanI 306 GGTGCC G^G[CT][GA]CC BbsI 848 GTCTTC GAAGAC(2/6) BbvCI 340 GCTGAGG CCTCAGC(-5/-2) BpuAI 848 GTCTTC GAAGAC(2/6) BsaI 360 GGTCTC GGTCTC(1/5) BsgI 900 CTGCAC GTGCAG(16/14) BsrBI 821 CCGCTC CCGCTC(-3/-3) BsrFI 52 GCCGGC [GA]^CCGG[CT] BstAPI 318 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 66 CGCG CG^CG Bsu36I 685 CCTCAGG CC^T[ACGT]AGG Cfr10I 52 GCCGGC [GA]^CCGG[CT] EagI 699 CGGCCG C^GGCCG EarI 391 CTCTTC CTCTTC(1/4) EclXI 699 CGGCCG C^GGCCG Eco52I 699 CGGCCG C^GGCCG Eco81I 685 CCTCAGG CC^T[ACGT]AGG EcoNI 248 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Ksp632I 391 CTCTTC CTCTTC(1/4) MaeII 20 ACGT A^CGT MfeI 995 CAATTG C^AATTG MslI 460 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 995 CAATTG C^AATTG MvnI 66 CGCG CG^CG NaeI 52 GCCGGC GCC^GGC NgoMIV 52 GCCGGC G^CCGGC PflMI 858 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 563 GAGTC GAGTC(4/5) PstI 964 CTGCAG CTGCA^G PvuI 47 CGATCG CGAT^CG SexAI 401 ACCTGGT A^CC[AT]GGT SmaI 827 CCCGGG CCC^GGG Tsp45I 92 GTGAC ^GT[GC]AC Van91I 858 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 827 CCCGGG C^CCGGG XmaI 827 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### BglI 648 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 732 GCCCAGCAGGC BpmI 838 CTGGAG CTGGAG(16/14) 880 CTGGAG BsiEI 47 CGATCG CG[GA][CT]^CG 699 CGGCCG BsmAI 361 GTCTC GTCTC(1/5) 637 GTCTC BsrI 497 ACTGG ACTGG(1/-1) 912 ACTGG BstF5I 460 CATCC GGATG(2/0) 336 GGATG BstXI 350 CCACCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 872 CCAAGGACCTGG BstYI 141 GGATCT [GA]^GATC[CT] 867 GGATCC CfoI 67 GCGC GCG^C 621 GCGC DraIII 400 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 859 CACCTTGTG EcoT14I 558 CCAAGG C^C[AT][AT]GG 872 CCAAGG FauI 354 CCCGC CCCGC(4/6) 111 GCGGG FokI 460 CATCC GGATG(9/13) 336 GGATG HhaI 67 GCGC GCG^C 621 GCGC HinP1I 67 GCGC G^CGC 621 GCGC MaeIII 92 GTGAC ^GT[ACGT]AC 238 GTTAC MflI 141 GGATCT [GA]^GATC[CT] 867 GGATCC NlaIII 89 CATG CATG^ 725 CATG PvuII 157 CAGCTG CAG^CTG 961 CAGCTG SfaNI 337 GATGC GCATC(5/9) 618 GATGC SfcI 594 CTACAG C^T[GA][CT]AG 964 CTGCAG StyI 558 CCAAGG C^C[AT][AT]GG 872 CCAAGG TfiI 674 GATTC G^A[AT]TC 814 GATTC Tsp509I 809 AATT ^AATT 996 AATT TspRI 296 CACTG CA[GC]TG[ACGT][ACGT]^ 641 CAGTG XhoII 141 GGATCT [GA]^GATC[CT] 867 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI TaqI TseI TthHB8I